Staphylococcus virus EW

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Phietavirus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4ZC14|Q4ZC14_9CAUD ORF026 OS=Staphylococcus virus EW OX=320841 PE=4 SV=1
MM1 pKa = 8.03KK2 pKa = 10.11IRR4 pKa = 11.84EE5 pKa = 4.35LNIDD9 pKa = 3.9DD10 pKa = 4.5RR11 pKa = 11.84VAFYY15 pKa = 10.72TDD17 pKa = 3.53SEE19 pKa = 4.41QEE21 pKa = 3.7KK22 pKa = 9.46PYY24 pKa = 10.09EE25 pKa = 4.35GKK27 pKa = 8.52VTEE30 pKa = 4.99LYY32 pKa = 11.25YY33 pKa = 10.78NFKK36 pKa = 9.86GKK38 pKa = 10.17EE39 pKa = 3.75MAQIEE44 pKa = 4.87LDD46 pKa = 3.63NAWYY50 pKa = 10.95YY51 pKa = 11.35NITDD55 pKa = 3.95DD56 pKa = 5.84DD57 pKa = 4.27DD58 pKa = 3.9WEE60 pKa = 4.45VIYY63 pKa = 10.68DD64 pKa = 3.63

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4ZC32|Q4ZC32_9CAUD ORF079 OS=Staphylococcus virus EW OX=320841 PE=4 SV=1
MM1 pKa = 7.03LQKK4 pKa = 10.39FRR6 pKa = 11.84IAKK9 pKa = 8.46EE10 pKa = 3.92KK11 pKa = 11.21NKK13 pKa = 10.68LKK15 pKa = 10.99LKK17 pKa = 9.14LLKK20 pKa = 10.2RR21 pKa = 11.84ASYY24 pKa = 10.27CLKK27 pKa = 9.86TSNNPEE33 pKa = 4.24LLRR36 pKa = 11.84AVAEE40 pKa = 4.04LLKK43 pKa = 10.76KK44 pKa = 10.35INRR47 pKa = 3.86

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

14315

35

975

185.9

21.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.547 ± 0.418

0.559 ± 0.087

6.322 ± 0.368

6.699 ± 0.39

4.115 ± 0.192

6.154 ± 0.389

1.802 ± 0.143

7.328 ± 0.208

8.543 ± 0.38

7.824 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.543 ± 0.191

6.455 ± 0.224

3.151 ± 0.27

4.191 ± 0.24

4.338 ± 0.228

5.994 ± 0.265

6.217 ± 0.334

6.594 ± 0.344

1.257 ± 0.178

4.366 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski