Minicystis rosea

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Myxococcales; Sorangiineae; Sorangiineae incertae sedis; Minicystis

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13965 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L6LUT3|A0A1L6LUT3_9DELT Uncharacterized protein OS=Minicystis rosea OX=888845 GN=A7982_10747 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 4.19ISIRR6 pKa = 11.84AICGGLGVAALVAACSANGGGNVFTSSDD34 pKa = 3.67GDD36 pKa = 3.58TGGAGHH42 pKa = 7.12GGSATGTKK50 pKa = 8.84STGTGAGDD58 pKa = 3.35VGGGDD63 pKa = 3.22ISFTTSGTGTGTGNTCNHH81 pKa = 6.48APDD84 pKa = 4.71ADD86 pKa = 3.92GDD88 pKa = 4.32GDD90 pKa = 3.61GWTANEE96 pKa = 4.67GDD98 pKa = 5.48CNDD101 pKa = 4.47CDD103 pKa = 4.24PNVNPGAIEE112 pKa = 4.31VVATDD117 pKa = 4.5PNAPPADD124 pKa = 4.13EE125 pKa = 5.25NCDD128 pKa = 3.37GMIDD132 pKa = 3.91NVEE135 pKa = 4.19PTCDD139 pKa = 4.43DD140 pKa = 3.85GLALDD145 pKa = 5.51DD146 pKa = 4.23VNPLNGARR154 pKa = 11.84AVDD157 pKa = 3.66LCKK160 pKa = 10.05IAEE163 pKa = 4.14EE164 pKa = 4.62TPASKK169 pKa = 10.24KK170 pKa = 10.63DD171 pKa = 3.56KK172 pKa = 8.39TWGVIGAQYY181 pKa = 10.65VRR183 pKa = 11.84ANGTAYY189 pKa = 10.02AAPGYY194 pKa = 9.93QVGLQAGWGPNVHH207 pKa = 6.34PQGGTKK213 pKa = 9.1MLAISSGRR221 pKa = 11.84ARR223 pKa = 11.84LPGQTGEE230 pKa = 4.9CGDD233 pKa = 3.94NSCPNNSPGQAPPGFPQDD251 pKa = 3.33VAGCDD256 pKa = 3.42GSTVIKK262 pKa = 10.61DD263 pKa = 3.63DD264 pKa = 3.34VALQVKK270 pKa = 9.65IRR272 pKa = 11.84TPTNATGYY280 pKa = 9.89SFAFNFYY287 pKa = 9.97SFEE290 pKa = 4.11YY291 pKa = 10.35PEE293 pKa = 4.55FVCTLFNDD301 pKa = 3.79QFISLVDD308 pKa = 3.57PAPNGSVSGNISFDD322 pKa = 4.02SNHH325 pKa = 5.91NPVSVNVAFFNVCDD339 pKa = 4.02PCPLGTAQLAGTGFDD354 pKa = 3.82GAWYY358 pKa = 9.74DD359 pKa = 4.65DD360 pKa = 3.84GGATGWLKK368 pKa = 10.79SQAPVKK374 pKa = 10.34GGDD377 pKa = 3.47VITIRR382 pKa = 11.84WTIWDD387 pKa = 4.26TGDD390 pKa = 3.85QSWDD394 pKa = 3.15STALVDD400 pKa = 4.07NFQWIANGGTVSVGTDD416 pKa = 4.32PINMPKK422 pKa = 10.47

Molecular weight: 42.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L6LM60|A0A1L6LM60_9DELT Uncharacterized protein OS=Minicystis rosea OX=888845 GN=A7982_08214 PE=4 SV=1
MM1 pKa = 7.53HH2 pKa = 7.62GAPSWSGRR10 pKa = 11.84GRR12 pKa = 11.84PARR15 pKa = 11.84SVLPSPPMIVWATLPSKK32 pKa = 10.66ARR34 pKa = 11.84PARR37 pKa = 11.84SAPPPRR43 pKa = 11.84SRR45 pKa = 11.84RR46 pKa = 11.84TPGKK50 pKa = 9.86RR51 pKa = 11.84RR52 pKa = 11.84AAAMAASRR60 pKa = 11.84PP61 pKa = 3.9

Molecular weight: 6.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13965

0

13965

4652819

14

7371

333.2

35.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.686 ± 0.041

1.319 ± 0.018

5.848 ± 0.017

5.681 ± 0.022

3.157 ± 0.012

8.959 ± 0.027

2.196 ± 0.012

3.915 ± 0.012

2.817 ± 0.018

9.524 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.868 ± 0.009

1.984 ± 0.015

6.322 ± 0.023

2.552 ± 0.011

7.994 ± 0.034

5.812 ± 0.019

5.491 ± 0.017

7.655 ± 0.018

1.297 ± 0.008

1.924 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski