Desulfotomaculum ferrireducens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfotomaculum

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2924 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S6IXZ2|A0A1S6IXZ2_9FIRM Uncharacterized protein OS=Desulfotomaculum ferrireducens OX=1833852 GN=B0537_11380 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.44AVVDD6 pKa = 3.61QDD8 pKa = 4.3LCISCGACIDD18 pKa = 3.69VCPEE22 pKa = 3.61VFDD25 pKa = 4.39WNDD28 pKa = 2.98EE29 pKa = 4.23EE30 pKa = 4.32KK31 pKa = 10.99AHH33 pKa = 6.83AIVDD37 pKa = 4.1EE38 pKa = 4.59VPDD41 pKa = 4.15GQEE44 pKa = 3.83EE45 pKa = 4.25QAKK48 pKa = 9.27EE49 pKa = 3.85AAEE52 pKa = 4.42GCPTDD57 pKa = 4.84AIKK60 pKa = 10.5ISS62 pKa = 3.33

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S6IT43|A0A1S6IT43_9FIRM CarD family transcriptional regulator OS=Desulfotomaculum ferrireducens OX=1833852 GN=B0537_01770 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.31QPKK8 pKa = 9.71KK9 pKa = 8.96RR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 4.85QKK14 pKa = 9.0VHH16 pKa = 5.85GFLKK20 pKa = 10.63RR21 pKa = 11.84MSTKK25 pKa = 10.29SGRR28 pKa = 11.84NVLKK32 pKa = 10.48RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.24GRR39 pKa = 11.84KK40 pKa = 8.9RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2924

0

2924

873544

25

1517

298.7

33.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.353 ± 0.056

1.222 ± 0.021

4.609 ± 0.033

6.926 ± 0.047

3.719 ± 0.033

7.496 ± 0.045

1.874 ± 0.022

7.075 ± 0.04

6.119 ± 0.039

10.619 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.574 ± 0.02

3.957 ± 0.029

4.212 ± 0.03

4.264 ± 0.031

4.942 ± 0.037

5.097 ± 0.03

5.231 ± 0.035

7.497 ± 0.038

0.959 ± 0.015

3.256 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski