Scophthalmus maximus (Turbot) (Psetta maxima)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; E

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24929 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U9CXE1|A0A2U9CXE1_SCOMX Putative zinc finger C2HC domain-containing protein 1A OS=Scophthalmus maximus OX=52904 GN=SMAX5B_002010 PE=4 SV=1
CC1 pKa = 7.78EE2 pKa = 5.95DD3 pKa = 4.11LDD5 pKa = 4.67DD6 pKa = 4.03WTSDD10 pKa = 3.23KK11 pKa = 11.01PPAVCHH17 pKa = 5.41SHH19 pKa = 6.54SLCQDD24 pKa = 3.33EE25 pKa = 5.21PVDD28 pKa = 3.99EE29 pKa = 4.92PVDD32 pKa = 3.93EE33 pKa = 4.85PVDD36 pKa = 4.05DD37 pKa = 4.84PVDD40 pKa = 3.81EE41 pKa = 4.92PVDD44 pKa = 3.85EE45 pKa = 4.88PVDD48 pKa = 3.62EE49 pKa = 5.21HH50 pKa = 8.46VDD52 pKa = 3.72EE53 pKa = 5.19PVDD56 pKa = 3.57EE57 pKa = 5.21HH58 pKa = 8.46VDD60 pKa = 3.72EE61 pKa = 5.19PVDD64 pKa = 3.57EE65 pKa = 5.21HH66 pKa = 8.46VDD68 pKa = 3.72EE69 pKa = 5.19PVDD72 pKa = 3.57EE73 pKa = 5.21HH74 pKa = 8.48VDD76 pKa = 3.81EE77 pKa = 5.32PVDD80 pKa = 3.84SSDD83 pKa = 4.13SFVIRR88 pKa = 11.84CGLFVSYY95 pKa = 10.39RR96 pKa = 11.84SSSSNEE102 pKa = 3.49

Molecular weight: 11.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U9CGN6|A0A2U9CGN6_SCOMX Putative TATA box-binding protein-associated factor RNA polymerase I subunit C OS=Scophthalmus maximus OX=52904 GN=SMAX5B_002962 PE=4 SV=1
FF1 pKa = 7.33NFRR4 pKa = 11.84FNFWFNFWFNFRR16 pKa = 11.84FNFWFNFRR24 pKa = 11.84FNFRR28 pKa = 11.84FNFWFNFRR36 pKa = 11.84FNFWFNFWFNFRR48 pKa = 11.84FNFWFNFWFNFRR60 pKa = 11.84FNFRR64 pKa = 11.84FNFRR68 pKa = 11.84FNFWFNFWFNFWFNFRR84 pKa = 11.84FNFRR88 pKa = 11.84FNFWFNFRR96 pKa = 11.84FNFRR100 pKa = 11.84FNFRR104 pKa = 11.84FNFWFNFWFNFWFNFWFNFRR124 pKa = 11.84FNFRR128 pKa = 11.84FNFWFNFRR136 pKa = 11.84FNFRR140 pKa = 11.84FNFRR144 pKa = 11.84FNFRR148 pKa = 11.84FNFRR152 pKa = 11.84FNFWFNFWFNFWFDD166 pKa = 3.18FRR168 pKa = 11.84FNFRR172 pKa = 11.84FNFTFNFWFNFWFNFRR188 pKa = 11.84FNFWFNFRR196 pKa = 11.84FNFWFNFSKK205 pKa = 10.99LKK207 pKa = 9.99TKK209 pKa = 10.76LNRR212 pKa = 11.84FNFWFNFWFNLWFNLWFNFKK232 pKa = 10.31FNCRR236 pKa = 11.84FKK238 pKa = 9.85QTVNFTVNRR247 pKa = 11.84LMASRR252 pKa = 11.84CTTLLNMM259 pKa = 5.21

Molecular weight: 36.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21118

3811

24929

14009638

43

29347

562.0

62.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.854 ± 0.014

2.243 ± 0.014

5.316 ± 0.012

6.915 ± 0.021

3.499 ± 0.012

6.434 ± 0.018

2.664 ± 0.008

4.136 ± 0.012

5.451 ± 0.019

9.474 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.37 ± 0.007

3.698 ± 0.009

5.71 ± 0.02

4.72 ± 0.019

5.943 ± 0.015

8.652 ± 0.019

5.638 ± 0.014

6.483 ± 0.015

1.145 ± 0.005

2.631 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski