Hoeflea halophila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Hoeflea

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3883 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286IFT3|A0A286IFT3_9RHIZ Uncharacterized protein OS=Hoeflea halophila OX=714899 GN=SAMN05877838_3072 PE=4 SV=1
MM1 pKa = 7.44SLFGMMQSGVSGMNAQANRR20 pKa = 11.84LSTVADD26 pKa = 4.22NIQNSSTPGYY36 pKa = 10.27KK37 pKa = 9.77RR38 pKa = 11.84ATTAFSSLILPTTAGSYY55 pKa = 10.83NSGAVKK61 pKa = 9.96TSVQYY66 pKa = 10.72DD67 pKa = 3.38ISTSGSPEE75 pKa = 3.61FTTSNTNLAIDD86 pKa = 3.79GNGFFIVSDD95 pKa = 4.02SNGSPFLTRR104 pKa = 11.84NGAFSKK110 pKa = 11.01DD111 pKa = 3.06GDD113 pKa = 4.11GNLVNSAGFTLMGYY127 pKa = 9.9DD128 pKa = 3.71YY129 pKa = 10.86TGGAPAPVVNGFDD142 pKa = 3.18GLVPVNVNSAGLTATPSTNGLFYY165 pKa = 11.43ANLNDD170 pKa = 3.78AATVVAAADD179 pKa = 3.96LPSANAVTAEE189 pKa = 4.03YY190 pKa = 10.19SHH192 pKa = 6.8KK193 pKa = 10.65SSLVVYY199 pKa = 7.84DD200 pKa = 3.74TVGKK204 pKa = 10.12EE205 pKa = 3.88VLLDD209 pKa = 3.74FYY211 pKa = 9.64YY212 pKa = 10.84TKK214 pKa = 10.29TADD217 pKa = 4.31DD218 pKa = 3.56NWEE221 pKa = 3.9VAVYY225 pKa = 10.29DD226 pKa = 4.24RR227 pKa = 11.84AAATPGTGFPYY238 pKa = 10.68GSGPLATQNLVFDD251 pKa = 4.56PALNGALAAASPTSIAMTVPGGAALTIDD279 pKa = 4.62LKK281 pKa = 11.75DD282 pKa = 3.51MTQLNYY288 pKa = 10.74EE289 pKa = 4.25FTVDD293 pKa = 4.14DD294 pKa = 4.81ADD296 pKa = 4.1VNGNAPSAVTDD307 pKa = 3.62VEE309 pKa = 4.51VAADD313 pKa = 3.49GTVYY317 pKa = 10.97AKK319 pKa = 10.97YY320 pKa = 10.55EE321 pKa = 4.2NGDD324 pKa = 4.07LRR326 pKa = 11.84PMYY329 pKa = 9.89QVALATVPSPDD340 pKa = 3.65NLIPLPGNVYY350 pKa = 10.64AQGIDD355 pKa = 3.25SGVITTGFAGTGGFGGLISNAVEE378 pKa = 3.99GSNVDD383 pKa = 3.01IAQEE387 pKa = 4.11LTDD390 pKa = 4.34MIASQRR396 pKa = 11.84SYY398 pKa = 9.73TANSKK403 pKa = 9.74VFQTGSDD410 pKa = 3.68LLDD413 pKa = 3.1VLVNLKK419 pKa = 10.3RR420 pKa = 3.6

Molecular weight: 43.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286IFD2|A0A286IFD2_9RHIZ Enolase OS=Hoeflea halophila OX=714899 GN=eno PE=3 SV=1
MM1 pKa = 7.62TKK3 pKa = 9.11RR4 pKa = 11.84TYY6 pKa = 10.36QPSKK10 pKa = 9.73LVRR13 pKa = 11.84KK14 pKa = 9.15RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.42GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.27GGRR29 pKa = 11.84KK30 pKa = 9.17VIQARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84NRR42 pKa = 11.84LSAA45 pKa = 3.96

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3883

0

3883

1229019

29

1822

316.5

34.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.966 ± 0.048

0.844 ± 0.012

5.833 ± 0.029

6.034 ± 0.038

3.846 ± 0.026

8.547 ± 0.034

2.022 ± 0.02

5.598 ± 0.025

3.522 ± 0.031

9.801 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.771 ± 0.018

2.796 ± 0.021

4.901 ± 0.027

3.035 ± 0.022

6.56 ± 0.039

5.841 ± 0.024

5.312 ± 0.024

7.288 ± 0.029

1.273 ± 0.014

2.212 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski