Staphylococcus phage Twort (strain DSM 17442 / HER 48) (Bacteriophage Twort)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Twortvirinae; Twortvirus; Staphylococcus virus Twort

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 195 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4Z954|Q4Z954_BPTWO ORF075 OS=Staphylococcus phage Twort (strain DSM 17442 / HER 48) OX=1283338 PE=4 SV=1
MM1 pKa = 7.83DD2 pKa = 6.27LISYY6 pKa = 9.88LEE8 pKa = 3.94NEE10 pKa = 4.2GFEE13 pKa = 4.25VSKK16 pKa = 10.35EE17 pKa = 3.71HH18 pKa = 7.17GYY20 pKa = 10.88EE21 pKa = 4.14GDD23 pKa = 3.78CYY25 pKa = 10.57LVKK28 pKa = 10.53SIEE31 pKa = 4.23GNEE34 pKa = 3.91VPIEE38 pKa = 4.38IISDD42 pKa = 3.65TVIITDD48 pKa = 4.24DD49 pKa = 3.88NGTDD53 pKa = 2.92IHH55 pKa = 8.34VDD57 pKa = 3.17ANKK60 pKa = 9.97PKK62 pKa = 10.67AIVDD66 pKa = 3.71VLLDD70 pKa = 3.8LTSTDD75 pKa = 3.64YY76 pKa = 11.18EE77 pKa = 4.23

Molecular weight: 8.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4Z9G0|Q4Z9G0_BPTWO ORF016 OS=Staphylococcus phage Twort (strain DSM 17442 / HER 48) OX=1283338 PE=4 SV=1
MM1 pKa = 8.05RR2 pKa = 11.84LRR4 pKa = 11.84NGYY7 pKa = 8.95ALKK10 pKa = 9.71TLSTSFNRR18 pKa = 11.84IYY20 pKa = 11.05KK21 pKa = 9.42KK22 pKa = 10.65LNRR25 pKa = 11.84KK26 pKa = 8.77FYY28 pKa = 10.84SMGHH32 pKa = 6.16IPMLFLLNIKK42 pKa = 9.68EE43 pKa = 4.03II44 pKa = 4.07

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

195

0

195

39026

33

1380

200.1

23.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.577 ± 0.236

0.725 ± 0.067

6.355 ± 0.134

8.005 ± 0.252

4.061 ± 0.117

5.448 ± 0.316

1.584 ± 0.083

7.334 ± 0.208

9.655 ± 0.283

8.561 ± 0.189

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.245 ± 0.096

7.034 ± 0.164

2.778 ± 0.176

3.546 ± 0.156

3.569 ± 0.119

6.665 ± 0.25

6.25 ± 0.216

6.442 ± 0.152

0.825 ± 0.061

5.343 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski