Sphaerochaeta globosa (strain ATCC BAA-1886 / DSM 22777 / Buddy) (Spirochaeta sp. (strain Buddy))

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Sphaerochaetaceae; Sphaerochaeta; Sphaerochaeta globosa

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3007 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0RY60|F0RY60_SPHGB ABC-type transporter integral membrane subunit OS=Sphaerochaeta globosa (strain ATCC BAA-1886 / DSM 22777 / Buddy) OX=158189 GN=SpiBuddy_0732 PE=3 SV=1
MM1 pKa = 7.08NTLKK5 pKa = 10.9NLTKK9 pKa = 10.75YY10 pKa = 10.4VFLFAIVGILSFAVGCSNDD29 pKa = 3.02TTLPINVTGITITGAADD46 pKa = 5.25AITVSNGEE54 pKa = 4.23TLQMSAAVLPLDD66 pKa = 3.97ATDD69 pKa = 5.78ASVTWSVASGSGTATISTTGLLTATAVGTVSVKK102 pKa = 9.76ATANDD107 pKa = 3.34DD108 pKa = 3.62SAYY111 pKa = 10.45EE112 pKa = 4.57GILTITVTAPNVAINIAAITGVTAPVRR139 pKa = 11.84SVTPDD144 pKa = 3.22LTVVEE149 pKa = 4.45TDD151 pKa = 3.17QYY153 pKa = 10.74TATITWAPSDD163 pKa = 3.74SPYY166 pKa = 11.09AGSTVYY172 pKa = 9.78TATITLAPKK181 pKa = 10.3AGYY184 pKa = 6.29TTTGVAADD192 pKa = 3.88AFTVSGADD200 pKa = 3.11TATNLVNSGVVTAVFPATSAAPLARR225 pKa = 11.84VEE227 pKa = 4.69LGTAADD233 pKa = 3.92YY234 pKa = 11.33VILAEE239 pKa = 4.33TLISTTGVTHH249 pKa = 4.74VTGDD253 pKa = 3.85LGISPYY259 pKa = 10.06ATTFITGFALVDD271 pKa = 3.3ATGYY275 pKa = 8.24ATSSLVTGSVYY286 pKa = 10.72AADD289 pKa = 4.11MADD292 pKa = 3.59PTPANLTTAVANMITAYY309 pKa = 10.45NDD311 pKa = 2.94AAARR315 pKa = 11.84PTPDD319 pKa = 3.71EE320 pKa = 4.71LNLGTGAIGGLILAPGLYY338 pKa = 9.75KK339 pKa = 9.85WDD341 pKa = 3.51GTVGIGADD349 pKa = 3.46LTLNGDD355 pKa = 4.56ANDD358 pKa = 2.84IWIFQISGDD367 pKa = 4.06LNMASDD373 pKa = 4.61FEE375 pKa = 4.4VLLTGGAKK383 pKa = 9.92PEE385 pKa = 4.24NIFWQVAGIATLGEE399 pKa = 4.12RR400 pKa = 11.84SHH402 pKa = 6.35MEE404 pKa = 3.84GVILSQTDD412 pKa = 2.94IVLQTGSSINGRR424 pKa = 11.84LLAQTQVTLDD434 pKa = 3.44AATVLEE440 pKa = 4.24PTILL444 pKa = 3.74

Molecular weight: 44.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0RVE8|F0RVE8_SPHGB Uncharacterized protein OS=Sphaerochaeta globosa (strain ATCC BAA-1886 / DSM 22777 / Buddy) OX=158189 GN=SpiBuddy_1115 PE=4 SV=1
MM1 pKa = 7.08NRR3 pKa = 11.84KK4 pKa = 9.08GLLPNLFIITLSYY17 pKa = 9.99FALGFVSILFANLALLCMVLPFILLAKK44 pKa = 9.91AKK46 pKa = 10.26KK47 pKa = 10.14KK48 pKa = 10.05LWCQTYY54 pKa = 9.98CPRR57 pKa = 11.84ASLLDD62 pKa = 3.71AAGKK66 pKa = 7.25KK67 pKa = 7.98TNWRR71 pKa = 11.84KK72 pKa = 9.57NPSLITSGKK81 pKa = 9.9LRR83 pKa = 11.84GIMLWYY89 pKa = 10.43FGLNLLFITGSTIQVAMLRR108 pKa = 11.84MEE110 pKa = 4.4AMQYY114 pKa = 10.06IRR116 pKa = 11.84LFIAIKK122 pKa = 10.46LFSLPQLITFQAPLFLMHH140 pKa = 7.02LSYY143 pKa = 10.94RR144 pKa = 11.84FYY146 pKa = 11.69GMMASTTILALVLSRR161 pKa = 11.84IYY163 pKa = 10.46RR164 pKa = 11.84PRR166 pKa = 11.84IWCAVCPIGTISDD179 pKa = 3.23ATLRR183 pKa = 11.84VQRR186 pKa = 11.84KK187 pKa = 6.58MKK189 pKa = 10.0PP190 pKa = 3.01

Molecular weight: 21.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3007

0

3007

1003326

35

2898

333.7

37.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.064 ± 0.042

1.223 ± 0.018

5.037 ± 0.035

6.103 ± 0.046

4.559 ± 0.033

6.976 ± 0.037

1.963 ± 0.02

6.686 ± 0.042

5.547 ± 0.037

10.931 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.75 ± 0.022

3.671 ± 0.028

3.781 ± 0.023

3.934 ± 0.029

4.525 ± 0.034

6.977 ± 0.04

5.668 ± 0.035

6.94 ± 0.038

1.035 ± 0.017

3.629 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski