Proteome-pI 2.0 - Proteome Isoelectric Point Database

Candidatus Baumannia cicadellinicola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; Candidatus Baumannia

Average proteome isoelectric point is 7.71

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 550 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A0K2BJY4|A0A0K2BJY4_9GAMM 50S ribosomal protein L34 OS=Candidatus Baumannia cicadellinicola OX=186490 GN=rpmH PE=3 SV=1

MM1 pKa = 7.93GITDD5 pKa = 5.61DD6 pKa = 3.97IFDD9 pKa = 3.64IARR12 pKa = 11.84SNKK15 pKa = 9.36EE16 pKa = 3.82DD17 pKa = 3.57LEE19 pKa = 5.19KK20 pKa = 11.13YY21 pKa = 10.15DD22 pKa = 4.04VLILGISTWYY32 pKa = 10.35YY33 pKa = 11.59GEE35 pKa = 4.6AQCDD39 pKa = 3.17WDD41 pKa = 4.05EE42 pKa = 4.73FFPTMKK48 pKa = 10.58QIDD51 pKa = 3.83FSGKK55 pKa = 8.83KK56 pKa = 9.34VALFGCGDD64 pKa = 3.44QEE66 pKa = 4.7DD67 pKa = 4.32YY68 pKa = 11.51SEE70 pKa = 4.54YY71 pKa = 10.82FCDD74 pKa = 4.91ALGTLGDD81 pKa = 4.2IIEE84 pKa = 4.31QQGATIVGHH93 pKa = 6.76WLTSSYY99 pKa = 11.26NFEE102 pKa = 4.01ASKK105 pKa = 11.3GLVNKK110 pKa = 10.42DD111 pKa = 3.42YY112 pKa = 11.1FIGLAIDD119 pKa = 4.07EE120 pKa = 4.62DD121 pKa = 4.16RR122 pKa = 11.84QPEE125 pKa = 4.14LTATRR130 pKa = 11.84VANWVKK136 pKa = 10.27QIKK139 pKa = 10.07YY140 pKa = 9.46EE141 pKa = 4.1LNIYY145 pKa = 9.57

Molecular weight: 16.6 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A0K2BK08|A0A0K2BK08_9GAMM Primosomal protein N' OS=Candidatus Baumannia cicadellinicola OX=186490 GN=priA PE=3 SV=1

MM1 pKa = 7.45KK2 pKa = 9.58RR3 pKa = 11.84TFQPSLLKK11 pKa = 10.58RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 7.46GFRR19 pKa = 11.84ARR21 pKa = 11.84IATKK25 pKa = 10.2NGRR28 pKa = 11.84KK29 pKa = 8.91VLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84IKK37 pKa = 10.55GRR39 pKa = 11.84SRR41 pKa = 11.84LTVSHH46 pKa = 7.0

Molecular weight: 5.47 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
550	0	550	177501	37	1407	322.7	36.35

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
6.627 ± 0.086	1.336 ± 0.038	4.495 ± 0.066	5.096 ± 0.091	3.844 ± 0.075	6.043 ± 0.079	2.179 ± 0.043	10.379 ± 0.115	6.648 ± 0.086	10.481 ± 0.102

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.515 ± 0.043	6.395 ± 0.088	3.419 ± 0.044	3.736 ± 0.062	4.69 ± 0.078	6.221 ± 0.061	5.253 ± 0.057	5.975 ± 0.086	1.007 ± 0.036	3.66 ± 0.055

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski