Stappia sp. ES.058

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Stappiaceae; Stappia; unclassified Stappia

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4219 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H2HD04|A0A1H2HD04_9RHOB ATP-binding cassette subfamily B multidrug efflux pump OS=Stappia sp. ES.058 OX=1881061 GN=SAMN05428979_2814 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84LHH4 pKa = 6.71HH5 pKa = 6.56LAFVAAVAAPLLTGSALAQGTGSEE29 pKa = 5.39GYY31 pKa = 10.63FEE33 pKa = 4.19VHH35 pKa = 6.5NNTEE39 pKa = 3.49AHH41 pKa = 5.43TVVGFYY47 pKa = 10.9TNDD50 pKa = 3.2GSGWSQNWLGDD61 pKa = 3.55IIDD64 pKa = 4.53PDD66 pKa = 3.7GSARR70 pKa = 11.84AEE72 pKa = 4.14FTSDD76 pKa = 4.18TGNCDD81 pKa = 2.81QTLRR85 pKa = 11.84VGWLGEE91 pKa = 4.48DD92 pKa = 3.35GTEE95 pKa = 4.31VLDD98 pKa = 4.04EE99 pKa = 5.37PISIDD104 pKa = 2.76ICAASDD110 pKa = 3.48IYY112 pKa = 11.4LDD114 pKa = 4.08DD115 pKa = 4.05NEE117 pKa = 5.24IYY119 pKa = 10.7FDD121 pKa = 3.58

Molecular weight: 13.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H2E2J8|A0A1H2E2J8_9RHOB Histidine kinase OS=Stappia sp. ES.058 OX=1881061 GN=SAMN05428979_0146 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 8.99RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.7GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.46NGRR28 pKa = 11.84QVILRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.64GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4219

0

4219

1348451

25

3580

319.6

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.697 ± 0.052

0.903 ± 0.014

6.126 ± 0.029

5.789 ± 0.036

3.772 ± 0.025

8.703 ± 0.044

2.02 ± 0.018

4.998 ± 0.029

2.974 ± 0.032

10.097 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.473 ± 0.02

2.43 ± 0.025

5.085 ± 0.032

2.806 ± 0.019

7.286 ± 0.043

5.472 ± 0.027

5.467 ± 0.032

7.552 ± 0.034

1.25 ± 0.013

2.101 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski