Wenzhou tombus-like virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KH35|A0A1L3KH35_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 9 OX=1923679 PE=4 SV=1
MM1 pKa = 7.31GMSNTSSSPNMQGQRR16 pKa = 11.84DD17 pKa = 3.46GSTRR21 pKa = 11.84VRR23 pKa = 11.84HH24 pKa = 6.03RR25 pKa = 11.84EE26 pKa = 3.76FITNVGAEE34 pKa = 4.19FGFDD38 pKa = 2.99TKK40 pKa = 11.12LYY42 pKa = 6.33TTGINPGDD50 pKa = 3.75GKK52 pKa = 9.09TFPWLSQLAKK62 pKa = 10.21GYY64 pKa = 9.91EE65 pKa = 4.11RR66 pKa = 11.84YY67 pKa = 9.39HH68 pKa = 6.15VNSMRR73 pKa = 11.84FEE75 pKa = 4.18YY76 pKa = 10.73EE77 pKa = 3.78PFSATTTSGTVSLLVDD93 pKa = 4.36YY94 pKa = 10.9DD95 pKa = 3.73PTDD98 pKa = 4.06PGPRR102 pKa = 11.84SKK104 pKa = 11.15SEE106 pKa = 3.83LLNSYY111 pKa = 9.98RR112 pKa = 11.84AVRR115 pKa = 11.84SSIWMSSHH123 pKa = 6.89TILSKK128 pKa = 11.47AEE130 pKa = 4.18LDD132 pKa = 3.83RR133 pKa = 11.84DD134 pKa = 3.36DD135 pKa = 4.7HH136 pKa = 7.34LFVRR140 pKa = 11.84TTSRR144 pKa = 11.84DD145 pKa = 3.44LMSEE149 pKa = 3.8NLKK152 pKa = 10.89LYY154 pKa = 10.64DD155 pKa = 4.16VGTLFCAFTDD165 pKa = 3.46IADD168 pKa = 3.34VSTLHH173 pKa = 6.49GEE175 pKa = 3.95LWVSYY180 pKa = 9.97DD181 pKa = 3.18ITLMIPSFHH190 pKa = 6.92LSSINNAEE198 pKa = 3.99VNALIVTDD206 pKa = 3.53SRR208 pKa = 11.84YY209 pKa = 10.21LGRR212 pKa = 11.84IDD214 pKa = 4.58PDD216 pKa = 3.28STANEE221 pKa = 4.3KK222 pKa = 10.74GSSLSYY228 pKa = 9.37STHH231 pKa = 6.52QIDD234 pKa = 3.66DD235 pKa = 3.3NAYY238 pKa = 9.19IEE240 pKa = 4.38FNEE243 pKa = 4.41PFSGMVQVSTRR254 pKa = 11.84TVSGDD259 pKa = 3.27LFQIEE264 pKa = 4.68PTLSPAAPISRR275 pKa = 11.84LAKK278 pKa = 9.63IGNAVSYY285 pKa = 9.11FVSNIDD291 pKa = 3.21TWLHH295 pKa = 4.18TVEE298 pKa = 4.56VVADD302 pKa = 3.85AGEE305 pKa = 4.0RR306 pKa = 11.84LAWRR310 pKa = 11.84IVDD313 pKa = 3.96QTAAWAGSLDD323 pKa = 4.26LLFTEE328 pKa = 4.78YY329 pKa = 11.11APALMGTLLALRR341 pKa = 11.84TDD343 pKa = 3.81EE344 pKa = 5.24AEE346 pKa = 3.91LTARR350 pKa = 11.84LQSDD354 pKa = 3.59HH355 pKa = 7.17LIGRR359 pKa = 11.84LPAPSSWTDD368 pKa = 2.11WRR370 pKa = 11.84TQHH373 pKa = 7.05GIPNSTTTEE382 pKa = 3.75THH384 pKa = 5.68

Molecular weight: 42.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KH35|A0A1L3KH35_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 9 OX=1923679 PE=4 SV=1
MM1 pKa = 7.5EE2 pKa = 4.76RR3 pKa = 11.84TLDD6 pKa = 3.84TEE8 pKa = 4.54SLDD11 pKa = 4.01TNSSKK16 pKa = 11.03VGSGCSQVSGLTSDD30 pKa = 5.43DD31 pKa = 3.61GVSCVGSSVASSEE44 pKa = 3.98EE45 pKa = 3.94SRR47 pKa = 11.84TGDD50 pKa = 4.54RR51 pKa = 11.84EE52 pKa = 4.21NRR54 pKa = 11.84WKK56 pKa = 10.87KK57 pKa = 8.39PVRR60 pKa = 11.84RR61 pKa = 11.84TVHH64 pKa = 5.97AVGAAATFGGIVLGCGKK81 pKa = 10.04IGLVLSPVTLGVMWALRR98 pKa = 11.84DD99 pKa = 3.76KK100 pKa = 10.92KK101 pKa = 10.27PSKK104 pKa = 10.5EE105 pKa = 3.7ADD107 pKa = 3.32LVLGVVNGDD116 pKa = 3.29IEE118 pKa = 4.47VEE120 pKa = 4.21KK121 pKa = 10.49EE122 pKa = 3.49ATLASTCEE130 pKa = 4.46GEE132 pKa = 4.12QQPRR136 pKa = 11.84KK137 pKa = 9.59IVGGGRR143 pKa = 11.84NDD145 pKa = 3.63AEE147 pKa = 4.32VVKK150 pKa = 9.73TKK152 pKa = 10.55KK153 pKa = 9.22KK154 pKa = 9.48QRR156 pKa = 11.84WYY158 pKa = 10.42QLKK161 pKa = 9.39KK162 pKa = 8.9QPKK165 pKa = 8.46RR166 pKa = 11.84RR167 pKa = 11.84IPVLAGEE174 pKa = 4.46VASGLKK180 pKa = 9.55VRR182 pKa = 11.84HH183 pKa = 6.0GLLADD188 pKa = 3.43NAEE191 pKa = 3.89NRR193 pKa = 11.84RR194 pKa = 11.84LIRR197 pKa = 11.84SDD199 pKa = 2.65ASRR202 pKa = 11.84RR203 pKa = 11.84CEE205 pKa = 3.62ALRR208 pKa = 11.84RR209 pKa = 11.84DD210 pKa = 3.17GDD212 pKa = 3.53EE213 pKa = 3.62WFKK216 pKa = 11.31NLRR219 pKa = 11.84NHH221 pKa = 7.26DD222 pKa = 3.7MLAVVVHH229 pKa = 6.49ASQMFWLMSDD239 pKa = 4.08DD240 pKa = 3.85EE241 pKa = 4.31EE242 pKa = 5.04AAISIYY248 pKa = 10.43DD249 pKa = 3.61DD250 pKa = 4.04HH251 pKa = 8.64YY252 pKa = 11.53LVANRR257 pKa = 11.84RR258 pKa = 11.84RR259 pKa = 11.84RR260 pKa = 11.84AKK262 pKa = 10.24LASSPTACC270 pKa = 4.99

Molecular weight: 29.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1211

270

557

403.7

45.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.193 ± 1.114

1.569 ± 0.481

6.936 ± 0.075

6.276 ± 0.403

3.716 ± 0.811

7.349 ± 0.497

2.395 ± 0.184

4.542 ± 0.449

5.037 ± 1.069

8.175 ± 0.69

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.725 ± 0.397

3.633 ± 0.194

4.377 ± 0.572

2.973 ± 0.351

7.845 ± 0.766

8.258 ± 1.377

5.945 ± 1.486

7.184 ± 0.743

1.734 ± 0.173

3.138 ± 0.613
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski