Cellulosilyticum lentocellum (strain ATCC 49066 / DSM 5427 / NCIMB 11756 / RHM5) (Clostridium lentocellum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Cellulosilyticum; Cellulosilyticum lentocellum

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4150 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2JPC1|F2JPC1_CELLD Recombinase OS=Cellulosilyticum lentocellum (strain ATCC 49066 / DSM 5427 / NCIMB 11756 / RHM5) OX=642492 GN=Clole_3165 PE=4 SV=1
MM1 pKa = 7.85KK2 pKa = 10.39KK3 pKa = 10.25RR4 pKa = 11.84ILSILLCFCMALSLLPMSALAADD27 pKa = 4.46DD28 pKa = 3.97PAVEE32 pKa = 4.16IGGTAVEE39 pKa = 4.27TGDD42 pKa = 3.29WYY44 pKa = 11.1ALTDD48 pKa = 3.53EE49 pKa = 4.97SGTVSTADD57 pKa = 3.41ANADD61 pKa = 2.94NWNVHH66 pKa = 4.4VTKK69 pKa = 10.54DD70 pKa = 3.62DD71 pKa = 3.42TDD73 pKa = 3.69TTVTVTLKK81 pKa = 10.86DD82 pKa = 3.22AAIANNSYY90 pKa = 8.58STHH93 pKa = 6.7AIDD96 pKa = 4.21VNGYY100 pKa = 9.09DD101 pKa = 4.64LEE103 pKa = 4.9IVLEE107 pKa = 4.42GTSNRR112 pKa = 11.84IGTGTTNDD120 pKa = 2.99NGYY123 pKa = 10.79AVYY126 pKa = 10.91NRR128 pKa = 11.84TDD130 pKa = 3.24GKK132 pKa = 10.75DD133 pKa = 3.08ITISGAGDD141 pKa = 3.43LTLTGYY147 pKa = 10.82YY148 pKa = 10.4GISTNGTGSVAVDD161 pKa = 3.06IDD163 pKa = 3.67GSLTVQSQWQMVSCGGQLNATAEE186 pKa = 4.58SITMNGYY193 pKa = 10.08YY194 pKa = 9.52IQCFGGGVSLSAEE207 pKa = 4.05NGDD210 pKa = 3.81VNISGTGDD218 pKa = 3.24YY219 pKa = 10.32GIKK222 pKa = 10.3VGTDD226 pKa = 2.6IMISVPKK233 pKa = 10.1GAVSISGGAYY243 pKa = 9.99SLYY246 pKa = 10.25TSVGNQISVYY256 pKa = 9.86AKK258 pKa = 10.32NDD260 pKa = 2.98ISLTEE265 pKa = 4.25CVQGGDD271 pKa = 3.84GNEE274 pKa = 4.19SQISLTSQTGDD285 pKa = 2.6ITVNSEE291 pKa = 4.02DD292 pKa = 3.61YY293 pKa = 10.75QAIAGVKK300 pKa = 8.94TYY302 pKa = 10.78LGLSAPEE309 pKa = 3.79GDD311 pKa = 2.98ITLTAEE317 pKa = 4.16SLGQNVIAGSNSYY330 pKa = 10.73ALNILAGGTLDD341 pKa = 4.68AQGQDD346 pKa = 3.63GIAGFGTAEE355 pKa = 3.8IKK357 pKa = 10.69AEE359 pKa = 4.13KK360 pKa = 10.54VNIAATGNGYY370 pKa = 10.75GMSGEE375 pKa = 4.46DD376 pKa = 3.29VSVTNPEE383 pKa = 4.06GGNCTEE389 pKa = 4.32ISISGGGDD397 pKa = 2.82GRR399 pKa = 11.84NALNTGNLTLKK410 pKa = 10.26ADD412 pKa = 4.14RR413 pKa = 11.84VIVAAASDD421 pKa = 3.6ASSAISAYY429 pKa = 10.48GNVNIGDD436 pKa = 3.74AGMIIGGTIVSNGTTTIADD455 pKa = 3.39GVLQVQASGPDD466 pKa = 3.36ISSSGLDD473 pKa = 3.94LNTLPAYY480 pKa = 8.34STYY483 pKa = 10.85YY484 pKa = 10.4KK485 pKa = 10.84AGDD488 pKa = 3.91GYY490 pKa = 11.85ALFTPAQNDD499 pKa = 3.72TPAVLTLHH507 pKa = 6.15NAEE510 pKa = 4.47ITSSDD515 pKa = 3.48TPLDD519 pKa = 3.92LGADD523 pKa = 4.14TIIKK527 pKa = 10.54LEE529 pKa = 4.06GSNYY533 pKa = 8.45LTNTNTEE540 pKa = 4.06SGVGIDD546 pKa = 5.02AGDD549 pKa = 3.7NSSGLPQSVIIQGGDD564 pKa = 3.17NDD566 pKa = 4.33SLYY569 pKa = 11.11VSAWQCTSSVEE580 pKa = 3.92NLIISGGKK588 pKa = 6.29VTMYY592 pKa = 9.96GSCYY596 pKa = 10.28GITDD600 pKa = 3.76TGNVLLEE607 pKa = 4.3NGAEE611 pKa = 4.16VSATGGEE618 pKa = 4.24YY619 pKa = 10.97GDD621 pKa = 4.06ALSVGFLEE629 pKa = 5.45DD630 pKa = 5.31DD631 pKa = 4.1EE632 pKa = 5.32NEE634 pKa = 4.11SAARR638 pKa = 11.84NLTINDD644 pKa = 3.74GSSLTAYY651 pKa = 9.43GYY653 pKa = 11.6VMIRR657 pKa = 11.84GNLVMSGEE665 pKa = 4.44SSSFTADD672 pKa = 3.16EE673 pKa = 4.08ISFVLGGLIIGSGSLLTVQPGRR695 pKa = 11.84EE696 pKa = 3.85FYY698 pKa = 11.1VPNSTSSVTNEE709 pKa = 3.33GTLLNNGTLLLPYY722 pKa = 9.51EE723 pKa = 4.31YY724 pKa = 10.86NAADD728 pKa = 3.6VAALNITGSGVVKK741 pKa = 9.96LYY743 pKa = 11.55SMGEE747 pKa = 3.89FPEE750 pKa = 4.13DD751 pKa = 3.0DD752 pKa = 3.51RR753 pKa = 11.84YY754 pKa = 10.82KK755 pKa = 10.88VYY757 pKa = 11.37VNGEE761 pKa = 4.25FYY763 pKa = 11.27ADD765 pKa = 3.44GGEE768 pKa = 4.25LNAQGSLDD776 pKa = 3.8LTTPPTEE783 pKa = 3.41ATYY786 pKa = 10.79YY787 pKa = 10.62KK788 pKa = 10.61SSADD792 pKa = 3.46DD793 pKa = 3.8AYY795 pKa = 9.98IIYY798 pKa = 9.23TPATDD803 pKa = 3.66SEE805 pKa = 4.54NAVLTLHH812 pKa = 6.48GMSNTGYY819 pKa = 8.85LQGGIVLPDD828 pKa = 3.28EE829 pKa = 4.62PVTVRR834 pKa = 11.84VEE836 pKa = 4.33GYY838 pKa = 10.72NEE840 pKa = 4.42IIWISVSKK848 pKa = 9.81EE849 pKa = 3.3IHH851 pKa = 5.25ITGSGRR857 pKa = 11.84LIGRR861 pKa = 11.84IEE863 pKa = 4.02NTASSAEE870 pKa = 3.96LTIDD874 pKa = 3.43SGVTASLMYY883 pKa = 9.22STKK886 pKa = 10.29TGDD889 pKa = 3.4VITNTVYY896 pKa = 11.24GDD898 pKa = 3.54YY899 pKa = 9.71STNAVYY905 pKa = 8.72VTAADD910 pKa = 4.21KK911 pKa = 11.01LVLMPGAVLTVNAGYY926 pKa = 11.08GEE928 pKa = 4.71LFFSEE933 pKa = 4.9GASLSDD939 pKa = 3.25MSIGTGASIVNNNYY953 pKa = 9.76IMLPQGTTAAQIASLPLSGTGIVRR977 pKa = 11.84VATAYY982 pKa = 10.41EE983 pKa = 4.58DD984 pKa = 4.04GSPTAWDD991 pKa = 3.9TYY993 pKa = 9.42TNDD996 pKa = 3.16GAAVKK1001 pKa = 10.41QIGDD1005 pKa = 3.78ADD1007 pKa = 3.92SGLDD1011 pKa = 3.55LTSGEE1016 pKa = 4.36HH1017 pKa = 6.01SGKK1020 pKa = 8.4TVEE1023 pKa = 4.16NDD1025 pKa = 3.29GYY1027 pKa = 11.6AWNSDD1032 pKa = 3.46SKK1034 pKa = 10.19TLTLGDD1040 pKa = 3.89AYY1042 pKa = 10.08IPGDD1046 pKa = 3.57LTLPSGTIVDD1056 pKa = 4.13TTDD1059 pKa = 2.82TTIIGGGLGGEE1070 pKa = 5.0AYY1072 pKa = 7.94TALNITLGGPAPLTISGGIRR1092 pKa = 11.84GGTNGDD1098 pKa = 3.29AVTVQGGAQVTVGDD1112 pKa = 5.05DD1113 pKa = 3.17IFLGASGGADD1123 pKa = 3.01GTLTVTGAGTQLNVSSPYY1141 pKa = 10.52GYY1143 pKa = 11.28AVMCDD1148 pKa = 3.49TVNVQSGASMTANGDD1163 pKa = 4.08SIGVEE1168 pKa = 3.7ALTGVNVTGGSTLTTNCDD1186 pKa = 3.13YY1187 pKa = 11.19GVYY1190 pKa = 10.16IIGGKK1195 pKa = 8.28LTVDD1199 pKa = 3.81DD1200 pKa = 4.19TSKK1203 pKa = 11.3LITNATIAPFCIVDD1217 pKa = 3.66STSNKK1222 pKa = 9.07SQSNVLALAGAPSGTVVASVTGTQAKK1248 pKa = 9.28YY1249 pKa = 9.19WSLVATGGTLSVTDD1263 pKa = 4.12EE1264 pKa = 4.52SNTPVTLSGARR1275 pKa = 11.84TGKK1278 pKa = 8.69LTFVKK1283 pKa = 10.43AATSGGNDD1291 pKa = 3.94DD1292 pKa = 5.36NGGGNNNNGGGASGSSTSYY1311 pKa = 10.24TLTFVTNGGTAISGVSKK1328 pKa = 11.08ASGTVVDD1335 pKa = 4.61LSGYY1339 pKa = 9.38KK1340 pKa = 8.39PTRR1343 pKa = 11.84EE1344 pKa = 4.42GYY1346 pKa = 10.69NFDD1349 pKa = 2.62GWYY1352 pKa = 10.52ADD1354 pKa = 3.52TALTTKK1360 pKa = 8.85VTSVTLTKK1368 pKa = 10.16GTTVYY1373 pKa = 11.04AKK1375 pKa = 8.41WTEE1378 pKa = 4.03KK1379 pKa = 9.9TVQSANPFVDD1389 pKa = 3.77VADD1392 pKa = 3.52SAYY1395 pKa = 11.11YY1396 pKa = 10.24HH1397 pKa = 7.32DD1398 pKa = 4.38AVLWATEE1405 pKa = 3.92KK1406 pKa = 11.05GITSGTTGTTFSPDD1420 pKa = 3.68KK1421 pKa = 10.54ICTRR1425 pKa = 11.84AQAVTFLWRR1434 pKa = 11.84AEE1436 pKa = 4.06GSPEE1440 pKa = 3.81PASANCPFTDD1450 pKa = 4.05VSADD1454 pKa = 3.31DD1455 pKa = 4.24YY1456 pKa = 11.41YY1457 pKa = 11.82YY1458 pKa = 11.18KK1459 pKa = 10.84AVLWAVEE1466 pKa = 3.89KK1467 pKa = 11.02GIVKK1471 pKa = 8.47GTSPTTFSPNDD1482 pKa = 3.45TVTRR1486 pKa = 11.84SQSMTFLWRR1495 pKa = 11.84AAGEE1499 pKa = 4.36TTSISANPFADD1510 pKa = 3.42VSKK1513 pKa = 10.89DD1514 pKa = 2.96AYY1516 pKa = 10.84YY1517 pKa = 11.31YY1518 pKa = 11.04NAVLWAVEE1526 pKa = 3.71KK1527 pKa = 10.79DD1528 pKa = 3.31ITKK1531 pKa = 8.85GTSDD1535 pKa = 3.52TAFSPDD1541 pKa = 3.42GGCTRR1546 pKa = 11.84AQIITLLYY1554 pKa = 10.31RR1555 pKa = 11.84YY1556 pKa = 8.29MGKK1559 pKa = 10.1

Molecular weight: 160.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2JSJ6|F2JSJ6_CELLD PSP1 domain protein OS=Cellulosilyticum lentocellum (strain ATCC 49066 / DSM 5427 / NCIMB 11756 / RHM5) OX=642492 GN=Clole_0014 PE=4 SV=1
MM1 pKa = 7.61AKK3 pKa = 10.41KK4 pKa = 10.4SMKK7 pKa = 10.03VKK9 pKa = 9.22QQRR12 pKa = 11.84PAKK15 pKa = 10.02FSTQAYY21 pKa = 5.18TRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PHH31 pKa = 6.8GYY33 pKa = 9.03LRR35 pKa = 11.84KK36 pKa = 10.07YY37 pKa = 9.32GICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.97LAYY49 pKa = 9.84KK50 pKa = 10.19GQIPGVKK57 pKa = 9.25KK58 pKa = 10.9ASWW61 pKa = 3.03

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4150

0

4150

1326943

30

3068

319.7

35.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.73 ± 0.039

1.231 ± 0.014

4.989 ± 0.031

7.734 ± 0.044

4.006 ± 0.025

6.666 ± 0.039

1.739 ± 0.018

8.506 ± 0.043

7.595 ± 0.032

9.421 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.84 ± 0.018

4.857 ± 0.028

3.116 ± 0.02

3.438 ± 0.02

3.493 ± 0.025

5.956 ± 0.032

5.788 ± 0.034

6.667 ± 0.033

0.889 ± 0.015

4.339 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski