Arthrobacter sp. Leaf69

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter; unclassified Arthrobacter

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3584 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S9BYT1|A0A0S9BYT1_9MICC ABC transmembrane type-1 domain-containing protein OS=Arthrobacter sp. Leaf69 OX=1736232 GN=ASE96_13375 PE=3 SV=1
MM1 pKa = 7.2PTGAKK6 pKa = 8.74HH7 pKa = 6.41QKK9 pKa = 8.76FTDD12 pKa = 3.4IEE14 pKa = 4.28DD15 pKa = 3.86EE16 pKa = 4.27PGISYY21 pKa = 11.1GEE23 pKa = 4.22DD24 pKa = 3.28CRR26 pKa = 11.84CTIGEE31 pKa = 4.27DD32 pKa = 3.71HH33 pKa = 7.28NSGDD37 pKa = 3.95DD38 pKa = 3.63TALSNYY44 pKa = 9.82LSEE47 pKa = 5.61SDD49 pKa = 6.02AEE51 pKa = 4.94DD52 pKa = 2.83IWLSSGMDD60 pKa = 3.19EE61 pKa = 6.22DD62 pKa = 4.61YY63 pKa = 11.46DD64 pKa = 4.01FRR66 pKa = 6.63

Molecular weight: 7.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S9CNK6|A0A0S9CNK6_9MICC Lipoprotein OS=Arthrobacter sp. Leaf69 OX=1736232 GN=ASE96_03000 PE=3 SV=1
MM1 pKa = 7.54AAMLGMLAGAGLLLIWWSAWEE22 pKa = 4.03RR23 pKa = 11.84PGKK26 pKa = 9.9FRR28 pKa = 11.84SAPRR32 pKa = 11.84RR33 pKa = 11.84SRR35 pKa = 11.84LEE37 pKa = 3.86DD38 pKa = 3.54LLRR41 pKa = 11.84TAGIEE46 pKa = 4.2KK47 pKa = 10.53VSGPGLLASCVGVGVFTTLVFFAVTKK73 pKa = 9.03SWPIAGCFGLFGGWLPMAIVKK94 pKa = 8.77WRR96 pKa = 11.84ARR98 pKa = 11.84KK99 pKa = 8.75RR100 pKa = 11.84AAVLRR105 pKa = 11.84QLWPDD110 pKa = 3.4VVDD113 pKa = 4.04HH114 pKa = 6.45LRR116 pKa = 11.84SAIRR120 pKa = 11.84AGLSLPEE127 pKa = 4.5ALIQLGDD134 pKa = 3.65KK135 pKa = 10.88GPEE138 pKa = 3.88EE139 pKa = 3.91LRR141 pKa = 11.84HH142 pKa = 4.86VFRR145 pKa = 11.84HH146 pKa = 5.83FGSDD150 pKa = 3.47YY151 pKa = 10.84RR152 pKa = 11.84SGGQFDD158 pKa = 4.12PSLTRR163 pKa = 11.84LKK165 pKa = 10.92DD166 pKa = 3.55RR167 pKa = 11.84LADD170 pKa = 3.73PVADD174 pKa = 5.02RR175 pKa = 11.84IVEE178 pKa = 4.04ALRR181 pKa = 11.84LTRR184 pKa = 11.84EE185 pKa = 4.13VGGSDD190 pKa = 3.9LGRR193 pKa = 11.84LLGTLAEE200 pKa = 4.53FLRR203 pKa = 11.84EE204 pKa = 4.06SARR207 pKa = 11.84TRR209 pKa = 11.84SEE211 pKa = 3.96LEE213 pKa = 3.8ARR215 pKa = 11.84QSWTVNAARR224 pKa = 11.84LAVAAPWIVLVLLASRR240 pKa = 11.84PEE242 pKa = 3.69AVAAYY247 pKa = 6.76NTPVGTAVLLGGLSVSLISYY267 pKa = 10.69AVMLRR272 pKa = 11.84IGALPEE278 pKa = 3.98DD279 pKa = 4.19EE280 pKa = 4.3RR281 pKa = 11.84VLRR284 pKa = 4.46

Molecular weight: 30.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3584

0

3584

1172066

37

3563

327.0

34.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.339 ± 0.056

0.595 ± 0.01

5.664 ± 0.031

5.612 ± 0.04

3.237 ± 0.027

9.201 ± 0.033

2.05 ± 0.016

4.317 ± 0.026

2.794 ± 0.033

10.286 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.009 ± 0.017

2.405 ± 0.021

5.482 ± 0.036

3.075 ± 0.022

6.562 ± 0.039

5.79 ± 0.024

5.802 ± 0.029

8.319 ± 0.032

1.41 ± 0.019

2.051 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski