Helicobacter phage FrMEG235U

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Schmidvirus; unclassified Schmidvirus

Average proteome isoelectric point is 7.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5RGH2|A0A1S5RGH2_9CAUD Uncharacterized protein OS=Helicobacter phage FrMEG235U OX=1852667 PE=4 SV=1
MM1 pKa = 7.66GIKK4 pKa = 9.82EE5 pKa = 4.23KK6 pKa = 10.55EE7 pKa = 3.99IEE9 pKa = 4.18LEE11 pKa = 3.99TLKK14 pKa = 11.02RR15 pKa = 11.84EE16 pKa = 3.96ITQAEE21 pKa = 4.07ASLEE25 pKa = 3.91QDD27 pKa = 4.59FIKK30 pKa = 10.97HH31 pKa = 5.29MVDD34 pKa = 2.81KK35 pKa = 10.6TNEE38 pKa = 3.95KK39 pKa = 10.6VEE41 pKa = 4.81DD42 pKa = 3.83LFFSNKK48 pKa = 8.85PEE50 pKa = 4.27FYY52 pKa = 10.1RR53 pKa = 11.84FVFTEE58 pKa = 3.6QNNYY62 pKa = 9.57LRR64 pKa = 11.84EE65 pKa = 4.0KK66 pKa = 10.26LTDD69 pKa = 3.66KK70 pKa = 10.75VSKK73 pKa = 11.02AMDD76 pKa = 3.95LSDD79 pKa = 4.78EE80 pKa = 4.11IQRR83 pKa = 11.84DD84 pKa = 3.19KK85 pKa = 11.29DD86 pKa = 3.41AEE88 pKa = 4.19EE89 pKa = 4.01IEE91 pKa = 4.13KK92 pKa = 11.03DD93 pKa = 3.3KK94 pKa = 11.44EE95 pKa = 4.14AFLKK99 pKa = 10.25KK100 pKa = 10.1HH101 pKa = 6.07PNIDD105 pKa = 3.86LNEE108 pKa = 4.16LLDD111 pKa = 4.19FYY113 pKa = 11.61NEE115 pKa = 4.28EE116 pKa = 3.61IPNRR120 pKa = 11.84IKK122 pKa = 10.86KK123 pKa = 10.37QIDD126 pKa = 3.48KK127 pKa = 11.34LEE129 pKa = 4.31GEE131 pKa = 4.6AFFEE135 pKa = 4.88AVLDD139 pKa = 3.85YY140 pKa = 11.18FNALNSKK147 pKa = 10.11PEE149 pKa = 4.02EE150 pKa = 4.21GQKK153 pKa = 10.54EE154 pKa = 4.18EE155 pKa = 4.64EE156 pKa = 4.49KK157 pKa = 11.35NNLPKK162 pKa = 10.42EE163 pKa = 4.0ALGNGVSGVGYY174 pKa = 10.77ANNEE178 pKa = 4.31NIMTRR183 pKa = 11.84YY184 pKa = 9.53

Molecular weight: 21.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5RGH4|A0A1S5RGH4_9CAUD DNA helicase OS=Helicobacter phage FrMEG235U OX=1852667 PE=4 SV=1
MM1 pKa = 7.67LLEE4 pKa = 4.74NGLLAILEE12 pKa = 4.05ALKK15 pKa = 10.55ILEE18 pKa = 4.36NKK20 pKa = 9.07SRR22 pKa = 11.84SVILEE27 pKa = 3.8RR28 pKa = 11.84LIIFYY33 pKa = 10.38IEE35 pKa = 4.17TQKK38 pKa = 11.16GQSDD42 pKa = 3.5EE43 pKa = 4.58KK44 pKa = 10.75AWQRR48 pKa = 11.84SQRR51 pKa = 11.84AYY53 pKa = 10.0KK54 pKa = 10.25RR55 pKa = 11.84SLVSKK60 pKa = 9.67AQKK63 pKa = 10.46NKK65 pKa = 10.3LKK67 pKa = 10.43RR68 pKa = 11.84KK69 pKa = 7.01QLEE72 pKa = 4.18RR73 pKa = 11.84VRR75 pKa = 11.84KK76 pKa = 8.31AQKK79 pKa = 10.26KK80 pKa = 9.77KK81 pKa = 10.56EE82 pKa = 4.0NQLNANRR89 pKa = 11.84AFSYY93 pKa = 10.02FEE95 pKa = 4.09RR96 pKa = 11.84PP97 pKa = 3.17

Molecular weight: 11.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35

0

35

9224

41

1943

263.5

30.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.873 ± 0.359

0.77 ± 0.212

4.879 ± 0.372

8.163 ± 0.418

4.987 ± 0.335

4.206 ± 0.328

1.507 ± 0.144

6.613 ± 0.236

10.776 ± 0.471

10.69 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.016 ± 0.183

7.903 ± 0.453

2.396 ± 0.199

4.781 ± 0.335

3.925 ± 0.273

6.613 ± 0.259

4.608 ± 0.236

4.196 ± 0.394

0.531 ± 0.082

3.567 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski