Mesorhizobium sp. B2-3-3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium; unclassified Mesorhizobium

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12366 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A504DPS0|A0A504DPS0_9RHIZ ActR/PrrA/RegA family redox response regulator transcription factor OS=Mesorhizobium sp. B2-3-3 OX=2589960 GN=FKO01_38735 PE=4 SV=1
SS1 pKa = 5.77EE2 pKa = 4.3TTTGSITYY10 pKa = 7.68TAPDD14 pKa = 3.85GPATVTIGGLAVSAVGQTFAGSFGTLTITSIAAGSVGYY52 pKa = 10.45SYY54 pKa = 10.34TLTTNTSGDD63 pKa = 3.54TTHH66 pKa = 7.63DD67 pKa = 3.71DD68 pKa = 3.7FAVSVTDD75 pKa = 4.02ADD77 pKa = 4.39GDD79 pKa = 4.17HH80 pKa = 6.97SDD82 pKa = 3.4KK83 pKa = 11.0TLTINIVDD91 pKa = 4.45DD92 pKa = 4.18VPTAHH97 pKa = 7.6ADD99 pKa = 3.69TDD101 pKa = 4.26SVTAGSYY108 pKa = 10.69GPEE111 pKa = 4.14LGNVITAVGTTSSGADD127 pKa = 3.14TLGADD132 pKa = 3.94GAVVAGVAAGSGGNLDD148 pKa = 3.5NAATVGAVIHH158 pKa = 5.69GTYY161 pKa = 11.02GDD163 pKa = 4.11LTLQANGDD171 pKa = 3.67YY172 pKa = 10.71SYY174 pKa = 11.38ARR176 pKa = 11.84HH177 pKa = 6.44AGTPGGVNDD186 pKa = 3.59VFTYY190 pKa = 8.39TLKK193 pKa = 11.21DD194 pKa = 3.22GDD196 pKa = 4.49GDD198 pKa = 4.17LSHH201 pKa = 6.69TTLTISIGDD210 pKa = 3.77SGVTTTVPVAGTAGAQVSEE229 pKa = 4.85AGLPPHH235 pKa = 6.78GALPAGSGEE244 pKa = 4.44SADD247 pKa = 3.72GLPNNNSDD255 pKa = 3.44TSEE258 pKa = 4.17TTTGSITYY266 pKa = 7.68TAPDD270 pKa = 3.85GPATVTIGGLAVSAVGQTFAGSFGTLTITSIAAGSVGYY308 pKa = 10.45SYY310 pKa = 10.34TLTTNTSGDD319 pKa = 3.54TTHH322 pKa = 7.63DD323 pKa = 3.71DD324 pKa = 3.7FAVSVTDD331 pKa = 4.02ADD333 pKa = 4.39GDD335 pKa = 4.17HH336 pKa = 6.97SDD338 pKa = 3.4KK339 pKa = 11.0TLTINIVDD347 pKa = 4.45DD348 pKa = 4.18VPTAHH353 pKa = 7.26ADD355 pKa = 3.05SWAPAITAPTVLTGLLGNDD374 pKa = 3.07VFGADD379 pKa = 5.05GIATTTPGMVTTTNGAHH396 pKa = 5.83GTVTYY401 pKa = 11.11NNDD404 pKa = 2.79GTFTYY409 pKa = 9.37TPTGIYY415 pKa = 9.86VGSDD419 pKa = 2.42SFTYY423 pKa = 9.66TIKK426 pKa = 11.15DD427 pKa = 3.27GDD429 pKa = 4.42GDD431 pKa = 3.96TSTATVTVNVQTNTVPGGGGTASLTLNEE459 pKa = 4.55AALDD463 pKa = 4.08TTQDD467 pKa = 3.36AADD470 pKa = 3.91LHH472 pKa = 6.89AGAVTGTNPTSPGEE486 pKa = 4.21SAQATSGITFTTTGEE501 pKa = 4.49AITVAFANPTGDD513 pKa = 4.26PNWVAPAVSGLAAGYY528 pKa = 9.49SISWALSGGQLVGTLMQGVTNLGPAIYY555 pKa = 9.75LALSNTSAGANSSLTPVVTATLTDD579 pKa = 3.9QLQHH583 pKa = 6.07TAGSGNITINGLQVVATDD601 pKa = 3.35TSGDD605 pKa = 3.86HH606 pKa = 6.85VSGAVNLTVLDD617 pKa = 4.44DD618 pKa = 4.23APLPFVGDD626 pKa = 4.31SISLPDD632 pKa = 3.88TLHH635 pKa = 6.02TALTEE640 pKa = 4.23HH641 pKa = 7.02LNFAPGAGADD651 pKa = 3.65GVGNVVFNVTTGAAVQDD668 pKa = 3.51ASGANVYY675 pKa = 11.03LNGEE679 pKa = 3.88QLFYY683 pKa = 10.77QVSADD688 pKa = 3.02GHH690 pKa = 4.7TVEE693 pKa = 5.53GRR695 pKa = 11.84SSVANGNDD703 pKa = 3.24LGFTATLNPATDD715 pKa = 2.88TWSFVLNGTIFNGSQFTTAGATATGGNNQVNAFTALTSPDD755 pKa = 3.78TPNDD759 pKa = 3.69LLVTANGSNNVNTSTGNFGVGSGQSITSGEE789 pKa = 4.24TIRR792 pKa = 11.84FDD794 pKa = 3.93FVTNASTTGTIAGTNYY810 pKa = 5.62TTHH813 pKa = 7.09YY814 pKa = 9.0EE815 pKa = 4.2VSSFTQGITKK825 pKa = 10.64ANGTVDD831 pKa = 2.99ITIRR835 pKa = 11.84AVNADD840 pKa = 3.62DD841 pKa = 5.61DD842 pKa = 4.23KK843 pKa = 11.63TFVGDD848 pKa = 3.93GSGEE852 pKa = 3.9ATATGITVTFVNGSGGAAPTLTNNGDD878 pKa = 3.73GTFTLHH884 pKa = 7.45GLDD887 pKa = 3.73QGDD890 pKa = 4.07TFTITASSDD899 pKa = 3.17PFSAVEE905 pKa = 3.65ISGASSGTFKK915 pKa = 11.06LGAASFATANAVDD928 pKa = 4.52PFDD931 pKa = 4.29INIPVTGTDD940 pKa = 3.53GDD942 pKa = 4.08SDD944 pKa = 3.91TAAGSVTAHH953 pKa = 7.55LYY955 pKa = 7.03PTATTLEE962 pKa = 4.28GTNGVDD968 pKa = 3.24NLVATAVKK976 pKa = 9.59TILLGQDD983 pKa = 3.33GDD985 pKa = 4.05DD986 pKa = 4.13HH987 pKa = 6.3LTGINGHH994 pKa = 5.8DD995 pKa = 3.95TILSGGQGNDD1005 pKa = 3.04TLTGLDD1011 pKa = 4.25GKK1013 pKa = 9.72DD1014 pKa = 3.42TLIGGAGADD1023 pKa = 3.76TMTGGAGNDD1032 pKa = 3.53TFVIGAGEE1040 pKa = 4.26STPVIGGSGNAGTISGYY1057 pKa = 10.7DD1058 pKa = 3.2IITDD1062 pKa = 4.14FVAGTDD1068 pKa = 3.47KK1069 pKa = 10.68LTLPGSLVAATSGNVNGGDD1088 pKa = 3.52STLTIGGDD1096 pKa = 3.76TVQSHH1101 pKa = 5.73SVTNGIASFFGTDD1114 pKa = 3.35SFTAPLAVTSAASLAAVVQYY1134 pKa = 11.25LNATDD1139 pKa = 3.82IGNAGATLAFTATISGVNHH1158 pKa = 6.48TYY1160 pKa = 10.79VYY1162 pKa = 9.58TQATTNAGVGALVDD1176 pKa = 4.18LQGVTISNLNTLIGGSVDD1194 pKa = 5.17PIILDD1199 pKa = 3.71LNHH1202 pKa = 6.65NGFAFSDD1209 pKa = 4.03VSHH1212 pKa = 6.57GVQFDD1217 pKa = 3.73MNGDD1221 pKa = 3.63GTKK1224 pKa = 10.32EE1225 pKa = 3.48QLAWNTSHH1233 pKa = 7.83DD1234 pKa = 3.57GMLAVDD1240 pKa = 5.14LNHH1243 pKa = 7.37DD1244 pKa = 3.81GKK1246 pKa = 11.14INDD1249 pKa = 3.8GTEE1252 pKa = 3.75LFTPNFGGGNFASGVAALASLDD1274 pKa = 4.04SNHH1277 pKa = 7.34DD1278 pKa = 3.4GVIDD1282 pKa = 3.97HH1283 pKa = 7.23NDD1285 pKa = 3.14AAFSSLLIWKK1295 pKa = 8.42DD1296 pKa = 3.32ANANGISDD1304 pKa = 4.02AGEE1307 pKa = 4.11LSHH1310 pKa = 7.16LADD1313 pKa = 3.96NGVVSISTAAHH1324 pKa = 6.05PAVGEE1329 pKa = 3.93IDD1331 pKa = 3.77GQTVTGNGTFQMADD1345 pKa = 3.39GTSGNYY1351 pKa = 9.42VEE1353 pKa = 5.55VEE1355 pKa = 3.88LDD1357 pKa = 3.41TSLVAPTQPSVAIDD1371 pKa = 3.71GTSGADD1377 pKa = 3.11TFKK1380 pKa = 10.64IDD1382 pKa = 3.58NLNIKK1387 pKa = 10.09DD1388 pKa = 4.9LISDD1392 pKa = 3.4YY1393 pKa = 11.19HH1394 pKa = 6.96GAEE1397 pKa = 3.94GDD1399 pKa = 4.19KK1400 pKa = 10.46IDD1402 pKa = 5.61LSALFDD1408 pKa = 3.81RR1409 pKa = 11.84APAGNIADD1417 pKa = 3.75YY1418 pKa = 11.1VHH1420 pKa = 6.56YY1421 pKa = 10.79NSATSTVSVDD1431 pKa = 3.33TSGSGNPANFVDD1443 pKa = 4.89VAVLQNAPPVGTINILYY1460 pKa = 10.11DD1461 pKa = 3.73DD1462 pKa = 4.33TNHH1465 pKa = 6.38AQHH1468 pKa = 6.61TATII1472 pKa = 4.02

Molecular weight: 146.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A504DH39|A0A504DH39_9RHIZ CDP-alcohol phosphatidyltransferase family protein OS=Mesorhizobium sp. B2-3-3 OX=2589960 GN=FKO01_40620 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ANLSAA45 pKa = 4.66

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12366

0

12366

3950924

21

7542

319.5

34.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.978 ± 0.036

0.783 ± 0.007

5.911 ± 0.018

5.623 ± 0.019

3.253 ± 0.015

9.176 ± 0.019

2.162 ± 0.012

4.153 ± 0.022

2.879 ± 0.022

10.088 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.114 ± 0.011

2.187 ± 0.012

5.598 ± 0.021

2.855 ± 0.012

7.446 ± 0.025

5.413 ± 0.016

5.784 ± 0.017

8.032 ± 0.021

1.416 ± 0.008

2.147 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski