Filifactor alocis (strain ATCC 35896 / D40 B5) (Fusobacterium alocis)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Filifactor; Filifactor alocis

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1616 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D6GR04|D6GR04_FILAD Uncharacterized protein OS=Filifactor alocis (strain ATCC 35896 / D40 B5) OX=546269 GN=HMPREF0389_00007 PE=4 SV=3
MM1 pKa = 7.68EE2 pKa = 5.23KK3 pKa = 10.59NKK5 pKa = 10.25NFLTEE10 pKa = 3.99EE11 pKa = 4.11EE12 pKa = 4.26NIKK15 pKa = 10.7INIVTEE21 pKa = 3.82NDD23 pKa = 3.27EE24 pKa = 4.24EE25 pKa = 4.47LSCEE29 pKa = 3.98VVGIYY34 pKa = 9.51EE35 pKa = 4.24AGEE38 pKa = 3.93YY39 pKa = 10.37EE40 pKa = 4.73YY41 pKa = 10.78IALVPDD47 pKa = 4.12NSDD50 pKa = 2.82EE51 pKa = 4.45LYY53 pKa = 10.42LYY55 pKa = 10.06RR56 pKa = 11.84YY57 pKa = 9.93EE58 pKa = 4.26EE59 pKa = 4.91GEE61 pKa = 4.78DD62 pKa = 3.82GLKK65 pKa = 10.32LDD67 pKa = 5.09SIEE70 pKa = 5.71DD71 pKa = 3.77LDD73 pKa = 3.96EE74 pKa = 4.27MKK76 pKa = 10.71LAEE79 pKa = 4.22EE80 pKa = 4.49VFWDD84 pKa = 3.71VFGTDD89 pKa = 4.04EE90 pKa = 4.23EE91 pKa = 5.18DD92 pKa = 3.44FDD94 pKa = 4.94EE95 pKa = 4.43KK96 pKa = 10.91HH97 pKa = 5.88HH98 pKa = 7.03HH99 pKa = 6.58CGCGCGCDD107 pKa = 3.83HH108 pKa = 6.8EE109 pKa = 4.78HH110 pKa = 6.93

Molecular weight: 12.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D6GT18|D6GT18_FILAD Competence protein CelA OS=Filifactor alocis (strain ATCC 35896 / D40 B5) OX=546269 GN=HMPREF0389_01255 PE=4 SV=1
MM1 pKa = 7.99DD2 pKa = 4.08KK3 pKa = 10.21MKK5 pKa = 9.89RR6 pKa = 11.84TYY8 pKa = 9.45QPKK11 pKa = 8.65RR12 pKa = 11.84RR13 pKa = 11.84QRR15 pKa = 11.84KK16 pKa = 8.14KK17 pKa = 7.85VHH19 pKa = 5.74GFRR22 pKa = 11.84KK23 pKa = 9.92RR24 pKa = 11.84MQSATGRR31 pKa = 11.84NVLRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84AKK40 pKa = 9.73GRR42 pKa = 11.84KK43 pKa = 8.85RR44 pKa = 11.84LSAA47 pKa = 3.96

Molecular weight: 5.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1616

0

1616

517034

37

3918

319.9

36.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.675 ± 0.064

1.146 ± 0.024

5.544 ± 0.06

8.126 ± 0.082

4.511 ± 0.047

6.377 ± 0.061

1.76 ± 0.026

8.417 ± 0.074

8.259 ± 0.07

9.067 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.922 ± 0.035

4.9 ± 0.047

2.966 ± 0.038

3.383 ± 0.039

3.924 ± 0.044

6.246 ± 0.048

5.177 ± 0.041

6.799 ± 0.056

0.722 ± 0.018

4.077 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski