Lepisosteus oculatus (Spotted gar)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Holostei; Semionotiformes; Lepisosteidae; Lepisosteus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22463 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5LV77|W5LV77_LEPOC Sulfate_transp domain-containing protein OS=Lepisosteus oculatus OX=7918 PE=4 SV=1
MM1 pKa = 7.24NLIAMILIITTILSCILAMVAFWLPQMVPDD31 pKa = 4.27SEE33 pKa = 4.42KK34 pKa = 10.81LSPYY38 pKa = 10.41EE39 pKa = 4.82CGFDD43 pKa = 3.65PLGSARR49 pKa = 11.84LPFSLRR55 pKa = 11.84FFLIAILFLLFDD67 pKa = 4.59LEE69 pKa = 4.35IALLLPLPWSDD80 pKa = 3.58QLTSPTTTLIWATVILMLLTLGLIYY105 pKa = 10.5EE106 pKa = 4.65WLQGGLEE113 pKa = 3.94WAEE116 pKa = 3.9

Molecular weight: 13.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5NES8|W5NES8_LEPOC Protein NLRC3-like OS=Lepisosteus oculatus OX=7918 PE=4 SV=1
QQ1 pKa = 7.44SSHH4 pKa = 4.14KK5 pKa = 8.58TFRR8 pKa = 11.84IKK10 pKa = 10.64RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18321

4142

22463

13406100

30

32359

596.8

66.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.664 ± 0.013

2.318 ± 0.015

5.014 ± 0.011

7.102 ± 0.022

3.706 ± 0.014

6.328 ± 0.022

2.505 ± 0.007

4.535 ± 0.014

5.886 ± 0.019

9.617 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.196 ± 0.007

3.811 ± 0.012

5.665 ± 0.022

4.74 ± 0.015

5.649 ± 0.014

8.509 ± 0.02

5.438 ± 0.012

6.31 ± 0.014

1.178 ± 0.006

2.817 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski