Clostridium acidisoli DSM 12555

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium acidisoli

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3815 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W1XA72|A0A1W1XA72_9CLOT Predicted PurR-regulated permease PerM OS=Clostridium acidisoli DSM 12555 OX=1121291 GN=SAMN02745134_01067 PE=3 SV=1
MM1 pKa = 7.7EE2 pKa = 4.43KK3 pKa = 7.92TTVIEE8 pKa = 4.02YY9 pKa = 10.55LKK11 pKa = 11.28SNDD14 pKa = 3.55FEE16 pKa = 4.46DD17 pKa = 4.3VEE19 pKa = 4.45EE20 pKa = 4.31IKK22 pKa = 11.3YY23 pKa = 10.33KK24 pKa = 10.73DD25 pKa = 3.15EE26 pKa = 4.08SLIVRR31 pKa = 11.84FYY33 pKa = 11.62YY34 pKa = 10.75DD35 pKa = 3.58FDD37 pKa = 4.83DD38 pKa = 5.27SEE40 pKa = 5.0IEE42 pKa = 4.03AASAYY47 pKa = 10.71ANDD50 pKa = 3.59EE51 pKa = 4.26CEE53 pKa = 4.22EE54 pKa = 4.04EE55 pKa = 4.58SEE57 pKa = 5.05GEE59 pKa = 3.1IWYY62 pKa = 10.22NKK64 pKa = 9.63FFIPYY69 pKa = 9.69LNDD72 pKa = 2.98IVVDD76 pKa = 3.74NVGEE80 pKa = 4.21IIEE83 pKa = 4.43NCSEE87 pKa = 4.01EE88 pKa = 4.32FDD90 pKa = 5.72LEE92 pKa = 4.32AQFITYY98 pKa = 10.19DD99 pKa = 3.2IDD101 pKa = 3.59EE102 pKa = 4.44EE103 pKa = 4.21QIEE106 pKa = 4.31YY107 pKa = 11.24AEE109 pKa = 4.89IIAEE113 pKa = 4.13FANRR117 pKa = 11.84GQEE120 pKa = 3.56IDD122 pKa = 3.6VEE124 pKa = 4.33KK125 pKa = 10.93AVDD128 pKa = 3.61DD129 pKa = 4.48LKK131 pKa = 11.13II132 pKa = 3.94

Molecular weight: 15.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W1XXR8|A0A1W1XXR8_9CLOT Stage V sporulation protein B OS=Clostridium acidisoli DSM 12555 OX=1121291 GN=SAMN02745134_03473 PE=4 SV=1
MM1 pKa = 7.46FMTYY5 pKa = 8.43QPKK8 pKa = 9.58KK9 pKa = 7.74KK10 pKa = 9.51QRR12 pKa = 11.84KK13 pKa = 8.04RR14 pKa = 11.84EE15 pKa = 3.69HH16 pKa = 6.26GFRR19 pKa = 11.84KK20 pKa = 9.97RR21 pKa = 11.84MNTPQGRR28 pKa = 11.84NVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.04GRR39 pKa = 11.84KK40 pKa = 8.99RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3815

0

3815

1156905

39

3064

303.3

34.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.691 ± 0.037

1.172 ± 0.016

5.516 ± 0.03

6.854 ± 0.05

4.501 ± 0.031

6.301 ± 0.039

1.369 ± 0.016

10.141 ± 0.042

9.133 ± 0.038

8.969 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.56 ± 0.018

6.598 ± 0.046

2.729 ± 0.025

2.388 ± 0.023

3.164 ± 0.028

6.592 ± 0.042

5.068 ± 0.031

6.405 ± 0.029

0.712 ± 0.013

4.138 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski