Proteus phage VB_PmiS-Isfahan

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gorganvirus; Proteus virus Isfahan

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U9ZAG3|A0A1U9ZAG3_9CAUD Uncharacterized protein OS=Proteus phage VB_PmiS-Isfahan OX=1969841 PE=4 SV=1
MM1 pKa = 7.84KK2 pKa = 10.36DD3 pKa = 3.03NAGVITNIDD12 pKa = 4.21DD13 pKa = 4.83IIEE16 pKa = 4.4TDD18 pKa = 3.68LTWYY22 pKa = 10.34KK23 pKa = 9.52VTIEE27 pKa = 4.16KK28 pKa = 10.57FEE30 pKa = 3.97EE31 pKa = 4.73PIYY34 pKa = 10.74YY35 pKa = 10.06LEE37 pKa = 5.13CEE39 pKa = 4.42LEE41 pKa = 4.22RR42 pKa = 11.84VNN44 pKa = 5.33

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U9ZA54|A0A1U9ZA54_9CAUD Large terminase subunit OS=Proteus phage VB_PmiS-Isfahan OX=1969841 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.49NKK4 pKa = 10.11IIALRR9 pKa = 11.84KK10 pKa = 9.82RR11 pKa = 11.84GFKK14 pKa = 10.27YY15 pKa = 11.03DD16 pKa = 4.16NICEE20 pKa = 4.06NLNVSKK26 pKa = 10.32MYY28 pKa = 9.28VAKK31 pKa = 10.37VLKK34 pKa = 10.1EE35 pKa = 3.91SGMTSNLWTKK45 pKa = 10.61EE46 pKa = 3.71LLQKK50 pKa = 10.89LEE52 pKa = 4.08EE53 pKa = 4.47LCNDD57 pKa = 4.05GYY59 pKa = 11.62SNAEE63 pKa = 3.76IAVKK67 pKa = 10.56LSVTHH72 pKa = 5.82AQVRR76 pKa = 11.84NAKK79 pKa = 9.46SKK81 pKa = 10.81HH82 pKa = 4.85GFKK85 pKa = 10.2QALPKK90 pKa = 10.14FKK92 pKa = 10.67KK93 pKa = 10.49DD94 pKa = 3.13IIEE97 pKa = 4.15LANIYY102 pKa = 10.49SSEE105 pKa = 3.91EE106 pKa = 3.62TANILKK112 pKa = 9.87LHH114 pKa = 6.47RR115 pKa = 11.84RR116 pKa = 11.84TVQAVRR122 pKa = 11.84TAYY125 pKa = 9.96GVNHH129 pKa = 6.55NKK131 pKa = 9.61RR132 pKa = 11.84YY133 pKa = 9.13YY134 pKa = 9.14MIRR137 pKa = 11.84RR138 pKa = 11.84AHH140 pKa = 6.44KK141 pKa = 10.13LYY143 pKa = 10.14NQGMSIDD150 pKa = 5.09DD151 pKa = 3.27ICKK154 pKa = 10.34ALNKK158 pKa = 9.92KK159 pKa = 8.75YY160 pKa = 8.77QTVEE164 pKa = 4.62DD165 pKa = 4.32YY166 pKa = 11.27LCTSINII173 pKa = 3.92

Molecular weight: 20.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

16554

39

1039

181.9

20.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.186 ± 0.523

1.498 ± 0.183

6.331 ± 0.212

6.989 ± 0.245

3.902 ± 0.17

6.476 ± 0.267

1.764 ± 0.193

7.177 ± 0.247

7.158 ± 0.243

8.173 ± 0.211

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.821 ± 0.119

6.192 ± 0.279

3.461 ± 0.213

3.256 ± 0.39

4.748 ± 0.186

6.156 ± 0.231

5.793 ± 0.241

6.482 ± 0.261

1.42 ± 0.132

4.017 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski