Aquirufa nivalisilvae

Taxonomy: cellular organisms

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2618 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S2DVC6|A0A2S2DVC6_9BACT Putative HTH-type transcriptional regulator OS=Aquirufa nivalisilvae OX=2516557 GN=HME7025_01372 PE=4 SV=1
MM1 pKa = 7.73AIIITDD7 pKa = 3.35EE8 pKa = 4.75CINCGACEE16 pKa = 4.07PEE18 pKa = 4.55CPNTAIYY25 pKa = 10.12EE26 pKa = 4.41GGVEE30 pKa = 4.13WTWAGGTALTEE41 pKa = 4.2VEE43 pKa = 4.72LGDD46 pKa = 4.84GSTMDD51 pKa = 3.62AKK53 pKa = 11.16APIEE57 pKa = 4.03PVSEE61 pKa = 3.85EE62 pKa = 3.91FYY64 pKa = 11.11YY65 pKa = 10.11IVPDD69 pKa = 3.36KK70 pKa = 10.65CTEE73 pKa = 3.83CTGFHH78 pKa = 7.08EE79 pKa = 5.07EE80 pKa = 4.13PQCAAVCPVDD90 pKa = 4.27CCVPDD95 pKa = 4.47PDD97 pKa = 4.74HH98 pKa = 8.76VEE100 pKa = 4.73DD101 pKa = 6.07KK102 pKa = 10.32DD103 pKa = 3.64TLLAKK108 pKa = 10.19KK109 pKa = 10.1AWLHH113 pKa = 6.44AEE115 pKa = 3.98

Molecular weight: 12.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S2DUN4|A0A2S2DUN4_9BACT Uncharacterized protein OS=Aquirufa nivalisilvae OX=2516557 GN=HME7025_01252 PE=4 SV=1
MM1 pKa = 7.73IKK3 pKa = 10.1NQTFRR8 pKa = 11.84IFWMFFIGTFLTVGMSSCWSARR30 pKa = 11.84CPRR33 pKa = 11.84EE34 pKa = 3.7TCRR37 pKa = 11.84VRR39 pKa = 11.84VEE41 pKa = 4.05HH42 pKa = 6.48RR43 pKa = 11.84HH44 pKa = 5.03GEE46 pKa = 4.01SYY48 pKa = 10.72YY49 pKa = 10.38RR50 pKa = 11.84PRR52 pKa = 11.84EE53 pKa = 3.98AFSWMWTPRR62 pKa = 11.84YY63 pKa = 9.73KK64 pKa = 10.27HH65 pKa = 6.03VRR67 pKa = 11.84TSNYY71 pKa = 8.68ATIGPNKK78 pKa = 7.9PTKK81 pKa = 9.86KK82 pKa = 9.24RR83 pKa = 11.84AWYY86 pKa = 9.89QFYY89 pKa = 10.84KK90 pKa = 10.24PADD93 pKa = 3.76RR94 pKa = 11.84VSSKK98 pKa = 10.65KK99 pKa = 10.09PRR101 pKa = 3.43

Molecular weight: 12.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2618

0

2618

959162

29

19489

366.4

40.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.338 ± 0.098

0.795 ± 0.02

5.078 ± 0.051

5.88 ± 0.115

5.056 ± 0.05

7.075 ± 0.11

2.011 ± 0.044

7.25 ± 0.08

6.851 ± 0.09

9.658 ± 0.098

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.379 ± 0.04

5.041 ± 0.056

3.979 ± 0.037

4.314 ± 0.06

3.65 ± 0.067

6.98 ± 0.119

5.439 ± 0.278

6.183 ± 0.06

1.349 ± 0.029

3.697 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski