Microbacterium phage Anakin

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Krampusvirus; unclassified Krampusvirus

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EH94|A0A4Y6EH94_9CAUD Uncharacterized protein OS=Microbacterium phage Anakin OX=2588136 GN=41 PE=4 SV=1
MM1 pKa = 7.68NIRR4 pKa = 11.84RR5 pKa = 11.84LVATAATALVVGGGLAVGTALPASAHH31 pKa = 5.54TPSVSATCSAIDD43 pKa = 3.57INLTNYY49 pKa = 7.61EE50 pKa = 4.31VKK52 pKa = 10.33PEE54 pKa = 4.18SGTPTIEE61 pKa = 4.0IANPDD66 pKa = 4.07YY67 pKa = 10.99VPEE70 pKa = 4.28VPGVPAQGTPTILVPNPDD88 pKa = 4.11YY89 pKa = 11.35VPAIPEE95 pKa = 4.04VPEE98 pKa = 4.03VLGDD102 pKa = 3.62PPLIKK107 pKa = 10.41AEE109 pKa = 3.92QLEE112 pKa = 4.61VSHH115 pKa = 6.64TEE117 pKa = 3.65NEE119 pKa = 4.08YY120 pKa = 10.46KK121 pKa = 10.31QWVTGKK127 pKa = 10.14LKK129 pKa = 9.7WVKK132 pKa = 10.71SDD134 pKa = 3.14DD135 pKa = 3.65WNPGFGWYY143 pKa = 8.02ATGNQRR149 pKa = 11.84IVVDD153 pKa = 4.0VPYY156 pKa = 10.73SPAVYY161 pKa = 10.01GPQPVITSYY170 pKa = 11.04QPAVPAVGEE179 pKa = 4.02PQIEE183 pKa = 4.56VANPAYY189 pKa = 10.37VPAVPAVPAVGEE201 pKa = 4.0PTKK204 pKa = 10.47TVEE207 pKa = 4.04NPDD210 pKa = 3.5YY211 pKa = 10.7VAADD215 pKa = 3.55ATPNTVTVIVDD226 pKa = 3.45GEE228 pKa = 4.34EE229 pKa = 4.06VLDD232 pKa = 4.01EE233 pKa = 4.4EE234 pKa = 5.69FGTSYY239 pKa = 11.18SNSVAIDD246 pKa = 3.3GTKK249 pKa = 8.57NHH251 pKa = 6.43SYY253 pKa = 9.87KK254 pKa = 10.52VEE256 pKa = 4.05VVGYY260 pKa = 8.19TGVGTKK266 pKa = 9.28TFTGKK271 pKa = 7.2TTACPPTPVVAPTLTVLPPTCDD293 pKa = 3.4ADD295 pKa = 3.78GSLPFLGNPAAQNPNGYY312 pKa = 8.88EE313 pKa = 4.09FPGQGYY319 pKa = 8.68RR320 pKa = 11.84VYY322 pKa = 10.82LDD324 pKa = 3.47KK325 pKa = 11.43SFTGAGTYY333 pKa = 9.78VATIQKK339 pKa = 9.52VGPGFDD345 pKa = 3.18PAFPYY350 pKa = 8.35GTKK353 pKa = 10.4VSGEE357 pKa = 4.25TKK359 pKa = 8.92QTLTVLPATGYY370 pKa = 10.61QGTDD374 pKa = 3.31PEE376 pKa = 4.64APCYY380 pKa = 10.8VPVPEE385 pKa = 4.23NTRR388 pKa = 11.84EE389 pKa = 4.1DD390 pKa = 4.66GEE392 pKa = 4.29WSTPIITCEE401 pKa = 4.0NEE403 pKa = 3.79VGDD406 pKa = 4.8EE407 pKa = 3.87ITITRR412 pKa = 11.84EE413 pKa = 3.82VTFTEE418 pKa = 4.17HH419 pKa = 6.15TLNTEE424 pKa = 3.91TGKK427 pKa = 10.25VEE429 pKa = 4.07TTTEE433 pKa = 4.08VVNEE437 pKa = 3.73NDD439 pKa = 3.44VYY441 pKa = 10.93IVTEE445 pKa = 3.87ADD447 pKa = 3.28IAEE450 pKa = 4.51LDD452 pKa = 4.18CPVVTPEE459 pKa = 3.97EE460 pKa = 4.51PEE462 pKa = 4.29TPSEE466 pKa = 4.13EE467 pKa = 4.43PEE469 pKa = 4.55TPTEE473 pKa = 4.24TPNAPTEE480 pKa = 4.47GNGTTLRR487 pKa = 11.84AATTQNDD494 pKa = 4.28AEE496 pKa = 4.45TLAQTGADD504 pKa = 4.08SPLPWVLGGTSLALGGLALWLFGIYY529 pKa = 9.61RR530 pKa = 11.84RR531 pKa = 11.84NQGGVVRR538 pKa = 11.84SGNPEE543 pKa = 3.47

Molecular weight: 57.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6ES99|A0A4Y6ES99_9CAUD Uncharacterized protein OS=Microbacterium phage Anakin OX=2588136 GN=2 PE=4 SV=1
MM1 pKa = 7.89PLLKK5 pKa = 9.67TLPTKK10 pKa = 10.42PGAYY14 pKa = 8.99MSSKK18 pKa = 10.82DD19 pKa = 3.33RR20 pKa = 11.84GIWLLEE26 pKa = 4.08AKK28 pKa = 10.26SDD30 pKa = 3.54GGLYY34 pKa = 9.61WRR36 pKa = 11.84MPGGFLMIDD45 pKa = 3.49SAARR49 pKa = 11.84SGPMSKK55 pKa = 10.08VVAEE59 pKa = 4.27HH60 pKa = 6.42LPFRR64 pKa = 11.84RR65 pKa = 11.84MLPSRR70 pKa = 11.84RR71 pKa = 3.69

Molecular weight: 7.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

17976

40

961

219.2

24.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.553 ± 0.482

0.729 ± 0.115

6.815 ± 0.322

7.699 ± 0.386

3.149 ± 0.164

7.766 ± 0.217

1.836 ± 0.138

4.918 ± 0.261

5.174 ± 0.296

7.304 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.559 ± 0.148

3.538 ± 0.233

4.918 ± 0.298

3.56 ± 0.161

6.197 ± 0.373

5.357 ± 0.19

6.364 ± 0.332

6.653 ± 0.294

2.008 ± 0.151

2.904 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski