Pangasianodon hypophthalmus (Striped catfish) (Helicophagus hypophthalmus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai;

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21221 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N5PX79|A0A5N5PX79_PANHP Protein kinase domain-containing protein OS=Pangasianodon hypophthalmus OX=310915 GN=PHYPO_G00105330 PE=4 SV=1
MM1 pKa = 7.88DD2 pKa = 4.9KK3 pKa = 10.99SFGMDD8 pKa = 4.64CIIQNPQYY16 pKa = 10.52ISEE19 pKa = 4.28SSVNEE24 pKa = 4.01DD25 pKa = 2.8QMEE28 pKa = 5.09GYY30 pKa = 10.48FSDD33 pKa = 3.98SPTFNSSVCDD43 pKa = 3.66SSDD46 pKa = 3.59EE47 pKa = 4.35EE48 pKa = 4.08ISTKK52 pKa = 10.39KK53 pKa = 10.32EE54 pKa = 3.27EE55 pKa = 4.15HH56 pKa = 6.32LRR58 pKa = 11.84LGPEE62 pKa = 4.32DD63 pKa = 5.19LNDD66 pKa = 3.79TVPYY70 pKa = 10.46DD71 pKa = 3.63YY72 pKa = 11.76

Molecular weight: 8.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N5JW18|A0A5N5JW18_PANHP CAP10 domain-containing protein OS=Pangasianodon hypophthalmus OX=310915 GN=PHYPO_G00152360 PE=4 SV=1
MM1 pKa = 7.43EE2 pKa = 5.61RR3 pKa = 11.84PRR5 pKa = 11.84LMRR8 pKa = 11.84LRR10 pKa = 11.84RR11 pKa = 11.84LQRR14 pKa = 11.84QCRR17 pKa = 11.84SQRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84LRR25 pKa = 11.84PSSIPPVHH33 pKa = 6.75PFLFFLSPSVRR44 pKa = 11.84PFLPSLHH51 pKa = 6.71PSIPLSPSLSLPLFPLFQQVSSPRR75 pKa = 11.84RR76 pKa = 11.84TPLSTNRR83 pKa = 11.84HH84 pKa = 4.29QRR86 pKa = 11.84NIFINNFVNTGSGFF100 pKa = 3.39

Molecular weight: 11.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21221

0

21221

12012490

53

25893

566.1

63.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.564 ± 0.015

2.269 ± 0.013

5.149 ± 0.012

7.157 ± 0.023

3.521 ± 0.01

6.11 ± 0.025

2.688 ± 0.009

4.409 ± 0.013

5.769 ± 0.018

9.475 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.393 ± 0.006

3.85 ± 0.012

5.58 ± 0.022

4.755 ± 0.017

5.648 ± 0.015

8.769 ± 0.022

5.663 ± 0.014

6.355 ± 0.014

1.148 ± 0.006

2.727 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski