Haloarcula hispanica virus SH1

Taxonomy: Viruses; Varidnaviria; Helvetiavirae; Dividoviricota; Laserviricetes; Halopanivirales; Sphaerolipoviridae; Alphasphaerolipovirus

Average proteome isoelectric point is 5.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4KPD5|Q4KPD5_9VIRU ORF 52 OS=Haloarcula hispanica virus SH1 OX=326574 PE=4 SV=1
MM1 pKa = 7.75TDD3 pKa = 3.63DD4 pKa = 4.87DD5 pKa = 5.67APGEE9 pKa = 4.57TPGTTDD15 pKa = 5.34DD16 pKa = 3.9STDD19 pKa = 3.8DD20 pKa = 4.22LDD22 pKa = 4.29HH23 pKa = 7.04LRR25 pKa = 11.84DD26 pKa = 3.68DD27 pKa = 3.86VRR29 pKa = 11.84RR30 pKa = 11.84SARR33 pKa = 11.84RR34 pKa = 11.84TLDD37 pKa = 3.36RR38 pKa = 11.84EE39 pKa = 4.39CPEE42 pKa = 4.93DD43 pKa = 4.35DD44 pKa = 4.7CDD46 pKa = 3.98ATEE49 pKa = 4.22HH50 pKa = 7.26ALFQQYY56 pKa = 9.18PAVLTTGAWGKK67 pKa = 9.74YY68 pKa = 8.39LNPVYY73 pKa = 10.3RR74 pKa = 11.84CQGCEE79 pKa = 3.53TAHH82 pKa = 6.47TLLCYY87 pKa = 8.81EE88 pKa = 4.65TDD90 pKa = 3.43QDD92 pKa = 4.04ADD94 pKa = 3.94RR95 pKa = 11.84PEE97 pKa = 3.93WGAYY101 pKa = 9.25PPEE104 pKa = 4.32QADD107 pKa = 3.75SEE109 pKa = 4.41AGEE112 pKa = 4.38GLDD115 pKa = 4.18AA116 pKa = 6.26

Molecular weight: 12.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4KPD2|Q4KPD2_9VIRU ORF 55 OS=Haloarcula hispanica virus SH1 OX=326574 PE=4 SV=1
MM1 pKa = 7.15VRR3 pKa = 11.84PSDD6 pKa = 3.59RR7 pKa = 11.84AVRR10 pKa = 11.84PAGGLLGVSPPRR22 pKa = 11.84IPFSPLRR29 pKa = 11.84QIPPSLNPPGGQTSGPTEE47 pKa = 3.77AARR50 pKa = 11.84RR51 pKa = 11.84TARR54 pKa = 11.84HH55 pKa = 6.12RR56 pKa = 11.84PRR58 pKa = 11.84QPRR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84NPHH67 pKa = 6.24LGLL70 pKa = 4.37

Molecular weight: 7.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

9928

37

1390

177.3

19.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.667 ± 0.785

0.987 ± 0.213

10.506 ± 0.578

8.239 ± 0.511

2.8 ± 0.276

9.861 ± 0.659

1.763 ± 0.214

2.518 ± 0.257

2.256 ± 0.222

6.668 ± 0.368

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.571 ± 0.205

2.619 ± 0.303

5.137 ± 0.348

3.676 ± 0.317

7.021 ± 0.46

5.409 ± 0.354

6.386 ± 0.296

7.514 ± 0.347

1.501 ± 0.153

2.901 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski