Caldisphaera lagunensis (strain DSM 15908 / JCM 11604 / ANMR 0165 / IC-154)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Acidilobales; Caldisphaeraceae; Caldisphaera; Caldisphaera lagunensis

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1477 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0A9F8|L0A9F8_CALLD Membrane-bound serine protease (ClpP class) OS=Caldisphaera lagunensis (strain DSM 15908 / JCM 11604 / ANMR 0165 / IC-154) OX=1056495 GN=Calag_0762 PE=4 SV=1
MM1 pKa = 7.25TCYY4 pKa = 10.11IDD6 pKa = 4.58LDD8 pKa = 4.01NVNDD12 pKa = 4.07LDD14 pKa = 5.01EE15 pKa = 6.4ISDD18 pKa = 3.85EE19 pKa = 4.44LNDD22 pKa = 3.68FAEE25 pKa = 5.64DD26 pKa = 3.01ISTIQDD32 pKa = 3.53YY33 pKa = 11.17LDD35 pKa = 3.17EE36 pKa = 5.35HH37 pKa = 6.48MGDD40 pKa = 4.6FIEE43 pKa = 5.24KK44 pKa = 10.4LNEE47 pKa = 3.94ALNSEE52 pKa = 4.78GIVLSCNEE60 pKa = 3.82AKK62 pKa = 10.2EE63 pKa = 4.44GNMEE67 pKa = 4.38CKK69 pKa = 10.58YY70 pKa = 10.69KK71 pKa = 10.66FQSDD75 pKa = 4.74LIGGEE80 pKa = 4.18VIFLVEE86 pKa = 5.26IDD88 pKa = 4.16PFDD91 pKa = 4.63NKK93 pKa = 10.52IKK95 pKa = 10.74DD96 pKa = 3.55YY97 pKa = 11.11DD98 pKa = 3.39IDD100 pKa = 4.41KK101 pKa = 10.76IRR103 pKa = 11.84LITKK107 pKa = 9.48

Molecular weight: 12.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0A8L4|L0A8L4_CALLD 50S ribosomal protein L15e OS=Caldisphaera lagunensis (strain DSM 15908 / JCM 11604 / ANMR 0165 / IC-154) OX=1056495 GN=rpl15e PE=3 SV=1
MM1 pKa = 7.58ARR3 pKa = 11.84NKK5 pKa = 10.21HH6 pKa = 5.33LARR9 pKa = 11.84KK10 pKa = 9.14LRR12 pKa = 11.84LAKK15 pKa = 10.24AGKK18 pKa = 9.75SNEE21 pKa = 4.29SVPVWVVVKK30 pKa = 7.84TVRR33 pKa = 11.84KK34 pKa = 7.51FTRR37 pKa = 11.84NPKK40 pKa = 8.56SRR42 pKa = 11.84NWRR45 pKa = 11.84TSKK48 pKa = 10.98LKK50 pKa = 10.6VV51 pKa = 3.09

Molecular weight: 5.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1477

0

1477

442436

32

1405

299.6

33.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.689 ± 0.058

0.618 ± 0.019

4.848 ± 0.055

6.681 ± 0.073

4.099 ± 0.054

6.476 ± 0.059

1.228 ± 0.022

10.833 ± 0.078

8.346 ± 0.092

9.882 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.362 ± 0.024

6.203 ± 0.068

3.971 ± 0.047

1.96 ± 0.031

3.57 ± 0.057

7.021 ± 0.076

4.362 ± 0.058

5.998 ± 0.044

1.009 ± 0.024

4.844 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski