Carnobacterium inhibens subsp. gilichinskyi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Carnobacteriaceae; Carnobacterium; Carnobacterium inhibens

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5S9D6|U5S9D6_9LACT Tyrosine-protein kinase CpsD OS=Carnobacterium inhibens subsp. gilichinskyi OX=1266845 GN=Q783_06630 PE=3 SV=1
MM1 pKa = 7.54EE2 pKa = 4.96KK3 pKa = 10.53KK4 pKa = 8.46EE5 pKa = 4.1WKK7 pKa = 9.88RR8 pKa = 11.84GFIGLVSMSALLLAACGNGEE28 pKa = 4.12EE29 pKa = 4.49SNEE32 pKa = 4.18TGSTSDD38 pKa = 4.18DD39 pKa = 3.96GEE41 pKa = 4.68STTLTIDD48 pKa = 2.95VDD50 pKa = 3.84PRR52 pKa = 11.84YY53 pKa = 10.25SDD55 pKa = 3.68YY56 pKa = 11.59VNEE59 pKa = 4.86IIPAFEE65 pKa = 4.18EE66 pKa = 3.91EE67 pKa = 4.51HH68 pKa = 5.76NVKK71 pKa = 10.16IEE73 pKa = 3.5ISEE76 pKa = 4.02RR77 pKa = 11.84DD78 pKa = 3.48MFDD81 pKa = 4.2GMDD84 pKa = 4.55ALPLDD89 pKa = 4.52GPAEE93 pKa = 4.23IGSDD97 pKa = 3.34ILIAPYY103 pKa = 10.71DD104 pKa = 3.73RR105 pKa = 11.84VGTLSQQGHH114 pKa = 5.67LAEE117 pKa = 4.37VTLPDD122 pKa = 3.86DD123 pKa = 3.54EE124 pKa = 6.78RR125 pKa = 11.84YY126 pKa = 11.0DD127 pKa = 4.39EE128 pKa = 4.17MDD130 pKa = 3.12QRR132 pKa = 11.84QVTLDD137 pKa = 3.41GKK139 pKa = 10.86VYY141 pKa = 10.06GAPFVIEE148 pKa = 4.29SLVMYY153 pKa = 9.1YY154 pKa = 10.75NKK156 pKa = 10.52DD157 pKa = 3.69LIEE160 pKa = 4.06QAPEE164 pKa = 3.93TFDD167 pKa = 4.53DD168 pKa = 5.41LEE170 pKa = 4.9ALSEE174 pKa = 4.5DD175 pKa = 3.37DD176 pKa = 4.04TYY178 pKa = 11.99AFEE181 pKa = 4.63NEE183 pKa = 4.13EE184 pKa = 4.17GKK186 pKa = 8.37NTAFLANWTTAYY198 pKa = 10.5QYY200 pKa = 10.79IGLLSGYY207 pKa = 9.22GGYY210 pKa = 10.53VFGEE214 pKa = 4.33NGTDD218 pKa = 3.52PSDD221 pKa = 3.24IGLNSPEE228 pKa = 4.15SVEE231 pKa = 4.32GITYY235 pKa = 8.32ITDD238 pKa = 3.47WYY240 pKa = 9.61QNVWPQGMLDD250 pKa = 3.5ATSSEE255 pKa = 3.83NFMNEE260 pKa = 3.61LFTSGKK266 pKa = 7.09TAAVINGPWGASGYY280 pKa = 10.36EE281 pKa = 3.83EE282 pKa = 5.47AGINYY287 pKa = 8.58GVSTIPTLPSGEE299 pKa = 4.27EE300 pKa = 3.95YY301 pKa = 10.81EE302 pKa = 4.28PFAGGVAWVISKK314 pKa = 10.18YY315 pKa = 10.88SKK317 pKa = 10.84NPEE320 pKa = 4.13LAQEE324 pKa = 3.95WLDD327 pKa = 3.53YY328 pKa = 9.11VTNAEE333 pKa = 4.08NSEE336 pKa = 4.17TLYY339 pKa = 10.86EE340 pKa = 4.16LTSEE344 pKa = 4.05IPANQEE350 pKa = 2.97ARR352 pKa = 11.84TTVSEE357 pKa = 4.28TGSEE361 pKa = 3.92LSKK364 pKa = 11.21AVIEE368 pKa = 4.41QFDD371 pKa = 4.08SAVPMPNIPEE381 pKa = 4.07MTEE384 pKa = 3.48VWTGAEE390 pKa = 3.97TMIFDD395 pKa = 4.37AASGNKK401 pKa = 9.49SPQQAADD408 pKa = 3.72DD409 pKa = 3.66AVQFIEE415 pKa = 4.32EE416 pKa = 4.38NIEE419 pKa = 3.75QKK421 pKa = 10.26YY422 pKa = 8.43QNN424 pKa = 3.73

Molecular weight: 46.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5SBZ6|U5SBZ6_9LACT 4-hydroxy-tetrahydrodipicolinate reductase OS=Carnobacterium inhibens subsp. gilichinskyi OX=1266845 GN=dapB PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 8.83KK9 pKa = 9.05RR10 pKa = 11.84KK11 pKa = 7.43RR12 pKa = 11.84QKK14 pKa = 8.87VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.83NGRR28 pKa = 11.84NVLQSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.58GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2269

0

2269

680895

29

1792

300.1

33.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.726 ± 0.065

0.533 ± 0.012

5.417 ± 0.04

7.85 ± 0.053

4.419 ± 0.039

6.476 ± 0.058

1.784 ± 0.024

8.07 ± 0.053

7.136 ± 0.052

9.696 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.707 ± 0.022

4.842 ± 0.04

3.28 ± 0.025

3.808 ± 0.031

3.544 ± 0.037

6.346 ± 0.046

6.017 ± 0.043

6.87 ± 0.038

0.846 ± 0.017

3.634 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski