Hubei unio douglasiae virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGQ4|A0A1L3KGQ4_9VIRU RNA-directed RNA polymerase OS=Hubei unio douglasiae virus 2 OX=1923322 PE=4 SV=1
MM1 pKa = 7.27FLKK4 pKa = 10.37DD5 pKa = 4.07DD6 pKa = 5.19KK7 pKa = 9.77YY8 pKa = 11.17HH9 pKa = 5.53EE10 pKa = 4.53WKK12 pKa = 10.08PVAPRR17 pKa = 11.84CIQYY21 pKa = 10.03RR22 pKa = 11.84GKK24 pKa = 10.69RR25 pKa = 11.84YY26 pKa = 9.77ALSLACYY33 pKa = 8.36LHH35 pKa = 7.2PIEE38 pKa = 4.37QMVYY42 pKa = 9.32GWTDD46 pKa = 3.29LSGTPIFAKK55 pKa = 10.31SRR57 pKa = 11.84NLTQRR62 pKa = 11.84GHH64 pKa = 7.42DD65 pKa = 3.38IADD68 pKa = 3.41KK69 pKa = 10.38MSYY72 pKa = 9.16FQNPVAISLDD82 pKa = 3.37HH83 pKa = 6.84SKK85 pKa = 10.9FDD87 pKa = 3.31AHH89 pKa = 8.2VNMSLLDD96 pKa = 3.86LEE98 pKa = 4.2HH99 pKa = 7.04WFYY102 pKa = 11.12KK103 pKa = 9.3QCNRR107 pKa = 11.84SPKK110 pKa = 10.54LKK112 pKa = 9.88MLLHH116 pKa = 6.07WQRR119 pKa = 11.84VNHH122 pKa = 5.86GQTKK126 pKa = 10.49NGTKK130 pKa = 10.11YY131 pKa = 7.79VTRR134 pKa = 11.84ATRR137 pKa = 11.84MSGDD141 pKa = 3.32QNTGIGNSIINYY153 pKa = 10.1AMTKK157 pKa = 10.66ALLEE161 pKa = 4.01KK162 pKa = 10.73LKK164 pKa = 10.33IRR166 pKa = 11.84HH167 pKa = 6.24CLYY170 pKa = 10.31IDD172 pKa = 3.92GDD174 pKa = 4.27DD175 pKa = 3.94FLVICEE181 pKa = 4.87RR182 pKa = 11.84IDD184 pKa = 3.3AGKK187 pKa = 10.22FDD189 pKa = 4.36PSLYY193 pKa = 9.96KK194 pKa = 10.38QFGMATKK201 pKa = 9.9LDD203 pKa = 4.46SITGVIEE210 pKa = 5.59HH211 pKa = 7.43IDD213 pKa = 3.6FCQCRR218 pKa = 11.84PVFNGSGYY226 pKa = 9.43TMVRR230 pKa = 11.84DD231 pKa = 4.91PYY233 pKa = 11.52RR234 pKa = 11.84MLQRR238 pKa = 11.84IQWAVGKK245 pKa = 9.37KK246 pKa = 9.71HH247 pKa = 6.11PRR249 pKa = 11.84HH250 pKa = 4.89VVNYY254 pKa = 6.82LTSIGKK260 pKa = 9.76CMIALGMGLPVEE272 pKa = 4.27QYY274 pKa = 10.39IGSTLSNLGGKK285 pKa = 10.05YY286 pKa = 8.35ITTEE290 pKa = 3.67QTMMANKK297 pKa = 9.54MFMRR301 pKa = 11.84PSNARR306 pKa = 11.84VVEE309 pKa = 4.18CCEE312 pKa = 3.85ASRR315 pKa = 11.84ASYY318 pKa = 8.47EE319 pKa = 4.17QAWGFTVGQQLILEE333 pKa = 4.58RR334 pKa = 11.84LSIGPPVLEE343 pKa = 4.77DD344 pKa = 3.69LVPFLQYY351 pKa = 10.92GSEE354 pKa = 4.08EE355 pKa = 4.46TVTEE359 pKa = 4.73GIRR362 pKa = 11.84PFSSSPIGSVYY373 pKa = 11.04

Molecular weight: 42.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGQ4|A0A1L3KGQ4_9VIRU RNA-directed RNA polymerase OS=Hubei unio douglasiae virus 2 OX=1923322 PE=4 SV=1
MM1 pKa = 7.66SSPTVKK7 pKa = 10.42SVVPNAGGNASVTGTGGLDD26 pKa = 3.12HH27 pKa = 6.99PTPGRR32 pKa = 11.84TGIRR36 pKa = 11.84ARR38 pKa = 11.84SGEE41 pKa = 4.16PGHH44 pKa = 6.49GRR46 pKa = 11.84NVCNRR51 pKa = 11.84KK52 pKa = 6.95TASRR56 pKa = 11.84RR57 pKa = 11.84NNEE60 pKa = 3.8EE61 pKa = 3.98PIKK64 pKa = 10.68RR65 pKa = 11.84SAPPNRR71 pKa = 11.84RR72 pKa = 11.84EE73 pKa = 4.19GRR75 pKa = 11.84DD76 pKa = 3.33KK77 pKa = 11.12RR78 pKa = 11.84PAGVPLGKK86 pKa = 10.6GKK88 pKa = 10.42GRR90 pKa = 11.84EE91 pKa = 4.22CGTLAPYY98 pKa = 10.38ARR100 pKa = 11.84SQQGRR105 pKa = 11.84APRR108 pKa = 11.84EE109 pKa = 3.75KK110 pKa = 10.49ASNQGFHH117 pKa = 6.94PKK119 pKa = 9.72EE120 pKa = 4.18DD121 pKa = 5.72PIPPWNRR128 pKa = 11.84VVNRR132 pKa = 11.84RR133 pKa = 11.84SDD135 pKa = 3.41RR136 pKa = 11.84AKK138 pKa = 10.97LLILIQQLNLRR149 pKa = 11.84IIQLEE154 pKa = 4.21RR155 pKa = 11.84EE156 pKa = 4.42AMRR159 pKa = 11.84LPSGQFQSAPILLARR174 pKa = 11.84NGWSTPASSFQASPKK189 pKa = 10.14LSRR192 pKa = 11.84NDD194 pKa = 2.84KK195 pKa = 10.07SEE197 pKa = 4.02RR198 pKa = 11.84KK199 pKa = 9.65RR200 pKa = 11.84EE201 pKa = 3.98VRR203 pKa = 11.84DD204 pKa = 3.64SNCEE208 pKa = 3.69VKK210 pKa = 10.87QNVLPPQPDD219 pKa = 3.23AQASGEE225 pKa = 4.3STAPQPTKK233 pKa = 10.06TPSPAGSRR241 pKa = 11.84KK242 pKa = 9.43SNRR245 pKa = 11.84GDD247 pKa = 3.15PTTPSSRR254 pKa = 11.84RR255 pKa = 11.84SRR257 pKa = 11.84KK258 pKa = 8.63SSYY261 pKa = 10.69GGSCEE266 pKa = 3.74TSAPFVGAKK275 pKa = 8.41TATEE279 pKa = 4.27PPSDD283 pKa = 3.34HH284 pKa = 6.9SSYY287 pKa = 10.94HH288 pKa = 6.57RR289 pKa = 11.84DD290 pKa = 3.16ISCTSAASTVSGVSRR305 pKa = 11.84CMSDD309 pKa = 2.9LTSEE313 pKa = 4.56EE314 pKa = 4.23KK315 pKa = 10.38PSSKK319 pKa = 9.86EE320 pKa = 3.79HH321 pKa = 6.04SRR323 pKa = 11.84KK324 pKa = 9.1PPKK327 pKa = 10.05NAGRR331 pKa = 11.84SYY333 pKa = 10.8QWTKK337 pKa = 10.84EE338 pKa = 4.1SGPMDD343 pKa = 4.68ANLDD347 pKa = 4.12PIYY350 pKa = 10.41GAEE353 pKa = 4.07VKK355 pKa = 10.03EE356 pKa = 4.23IPRR359 pKa = 11.84GRR361 pKa = 11.84VKK363 pKa = 10.74KK364 pKa = 10.57SIRR367 pKa = 11.84KK368 pKa = 7.36WNKK371 pKa = 4.52TTKK374 pKa = 10.1VLPTEE379 pKa = 4.43VEE381 pKa = 3.88LHH383 pKa = 6.49FYY385 pKa = 10.83LVLEE389 pKa = 4.5FAFVPRR395 pKa = 11.84DD396 pKa = 3.83CTVMRR401 pKa = 11.84QMTNKK406 pKa = 9.92AKK408 pKa = 10.57SYY410 pKa = 11.05LNTFDD415 pKa = 3.36TSNYY419 pKa = 6.94TAEE422 pKa = 3.86EE423 pKa = 4.12RR424 pKa = 11.84FRR426 pKa = 11.84LVMKK430 pKa = 10.35AVRR433 pKa = 11.84SAMCVTEE440 pKa = 4.35EE441 pKa = 4.3EE442 pKa = 4.44EE443 pKa = 4.56CVRR446 pKa = 11.84AALKK450 pKa = 11.05NPDD453 pKa = 3.71VQEE456 pKa = 4.38LAHH459 pKa = 6.4KK460 pKa = 9.83QAAFIQEE467 pKa = 4.02GFVGNEE473 pKa = 4.22GIGSVKK479 pKa = 10.45SYY481 pKa = 11.03LSNACKK487 pKa = 10.33INVFKK492 pKa = 11.03KK493 pKa = 9.47GTHH496 pKa = 5.59LPKK499 pKa = 10.17KK500 pKa = 10.22VKK502 pKa = 10.37

Molecular weight: 55.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1182

307

502

394.0

43.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.276 ± 0.898

1.946 ± 0.371

3.807 ± 0.417

5.076 ± 0.823

3.299 ± 0.502

7.783 ± 0.49

1.946 ± 0.557

4.315 ± 0.875

6.345 ± 1.016

6.514 ± 0.861

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.876 ± 0.711

4.653 ± 0.364

6.853 ± 0.964

4.315 ± 0.391

7.191 ± 1.054

8.883 ± 0.984

6.43 ± 0.725

6.176 ± 0.354

1.438 ± 0.379

2.876 ± 0.871
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski