Quadrisphaera granulorum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Kineosporiales; Kineosporiaceae; Quadrisphaera

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4581 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A315ZLT9|A0A315ZLT9_9ACTN Permease family protein OS=Quadrisphaera granulorum OX=317664 GN=BXY45_14612 PE=3 SV=1
MM1 pKa = 7.85RR2 pKa = 11.84EE3 pKa = 4.04AFLVPAVGLLAALALAGCSAPTEE26 pKa = 3.98AAAPAEE32 pKa = 4.36SPSVAEE38 pKa = 4.03SAAPAEE44 pKa = 4.18TAAAAEE50 pKa = 4.47GTDD53 pKa = 3.21LTEE56 pKa = 3.93QVRR59 pKa = 11.84AAVGEE64 pKa = 4.1DD65 pKa = 3.55LAPLVTSAEE74 pKa = 3.98QTEE77 pKa = 4.27PGRR80 pKa = 11.84VQVDD84 pKa = 3.45TSISDD89 pKa = 3.35PRR91 pKa = 11.84TDD93 pKa = 4.07DD94 pKa = 3.53SAEE97 pKa = 4.03AGQAIYY103 pKa = 10.29ICDD106 pKa = 3.65AVSGMDD112 pKa = 3.28AVEE115 pKa = 4.31HH116 pKa = 5.53VSVMEE121 pKa = 4.2SDD123 pKa = 3.21GSTFVVYY130 pKa = 8.26GHH132 pKa = 7.04PAYY135 pKa = 9.48PAGEE139 pKa = 4.18CSEE142 pKa = 4.35VV143 pKa = 3.11

Molecular weight: 14.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A316ADV1|A0A316ADV1_9ACTN 50S ribosomal protein L18 OS=Quadrisphaera granulorum OX=317664 GN=rplR PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.87GRR40 pKa = 11.84AQLSAA45 pKa = 3.87

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4581

0

4581

1470649

25

1900

321.0

33.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.181 ± 0.068

0.647 ± 0.009

5.985 ± 0.031

5.206 ± 0.036

2.31 ± 0.022

9.347 ± 0.034

2.125 ± 0.019

2.483 ± 0.027

1.382 ± 0.021

10.887 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.471 ± 0.013

1.316 ± 0.016

5.97 ± 0.029

2.884 ± 0.019

7.925 ± 0.035

5.665 ± 0.028

6.074 ± 0.03

10.068 ± 0.035

1.521 ± 0.015

1.553 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski