Gordonia insulae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Gordoniaceae; Gordonia

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5417 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8JHI7|A0A3G8JHI7_9ACTN Uncharacterized protein OS=Gordonia insulae OX=2420509 GN=D7316_01049 PE=4 SV=1
MM1 pKa = 7.7PRR3 pKa = 11.84PTLLEE8 pKa = 4.0FVTALAHH15 pKa = 6.89DD16 pKa = 4.09PAFAAAYY23 pKa = 7.22WADD26 pKa = 3.46PAEE29 pKa = 4.32TLRR32 pKa = 11.84DD33 pKa = 3.79ANLHH37 pKa = 6.14DD38 pKa = 4.43VSAADD43 pKa = 4.03ISALMPMAGGADD55 pKa = 3.38AGGLSTVGDD64 pKa = 4.0NVWRR68 pKa = 11.84GSEE71 pKa = 3.9ALLAFDD77 pKa = 4.75VVSDD81 pKa = 4.08DD82 pKa = 4.25AVPIATGQFSDD93 pKa = 4.0PAGLDD98 pKa = 3.76FAHH101 pKa = 6.83SAPEE105 pKa = 4.0PLDD108 pKa = 3.38TCEE111 pKa = 4.11FDD113 pKa = 4.74CVQTLSDD120 pKa = 4.52LYY122 pKa = 11.33SAIEE126 pKa = 3.73VDD128 pKa = 3.56AAVHH132 pKa = 6.26VDD134 pKa = 3.34TDD136 pKa = 3.74LTAQIDD142 pKa = 3.79AVDD145 pKa = 4.38DD146 pKa = 4.12VRR148 pKa = 11.84QHH150 pKa = 6.57PCAGWSTADD159 pKa = 4.2PGDD162 pKa = 3.64QAGDD166 pKa = 4.01LYY168 pKa = 11.57

Molecular weight: 17.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8JRP9|A0A3G8JRP9_9ACTN Oxidoreductase SadH OS=Gordonia insulae OX=2420509 GN=sadH_3 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84SRR17 pKa = 11.84VHH19 pKa = 6.33GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVNGRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.92GRR42 pKa = 11.84AKK44 pKa = 9.67LTAA47 pKa = 4.21

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5417

0

5417

1825819

30

15198

337.1

36.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.728 ± 0.043

0.733 ± 0.011

6.906 ± 0.036

5.161 ± 0.033

2.998 ± 0.018

9.037 ± 0.033

2.199 ± 0.016

4.606 ± 0.023

1.974 ± 0.028

9.437 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.049 ± 0.015

2.054 ± 0.02

5.618 ± 0.026

2.678 ± 0.016

7.356 ± 0.033

5.7 ± 0.021

6.405 ± 0.023

8.914 ± 0.036

1.434 ± 0.012

2.016 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski