Egibacter rhizosphaerae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Nitriliruptoria; Egibacterales; Egibacteraceae; Egibacter

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4050 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411YKG2|A0A411YKG2_9ACTN Transport permease protein OS=Egibacter rhizosphaerae OX=1670831 GN=ER308_20265 PE=3 SV=1
MM1 pKa = 7.79ASVQLNPYY9 pKa = 11.03LMFQDD14 pKa = 3.98RR15 pKa = 11.84ARR17 pKa = 11.84EE18 pKa = 3.84AMDD21 pKa = 3.82FYY23 pKa = 11.44RR24 pKa = 11.84EE25 pKa = 4.1VFGGEE30 pKa = 3.97LTMQTYY36 pKa = 10.99AEE38 pKa = 4.72GGMSADD44 pKa = 3.48PSNDD48 pKa = 2.8EE49 pKa = 5.02RR50 pKa = 11.84IMHH53 pKa = 6.33AQLVTPHH60 pKa = 6.45GLILMASDD68 pKa = 4.01VPDD71 pKa = 4.07GVPYY75 pKa = 10.55EE76 pKa = 4.31PGSAISISLSGDD88 pKa = 3.2DD89 pKa = 4.65GSALGGFYY97 pKa = 10.72DD98 pKa = 3.99ALVDD102 pKa = 3.93GGTVIQPLTEE112 pKa = 4.42APWGDD117 pKa = 3.54TFGVCVDD124 pKa = 3.92RR125 pKa = 11.84FGTSWMVNIGSAPTT139 pKa = 3.41

Molecular weight: 14.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411YCX3|A0A411YCX3_9ACTN Xanthine dehydrogenase family protein subunit M OS=Egibacter rhizosphaerae OX=1670831 GN=ER308_05740 PE=4 SV=1
MM1 pKa = 7.67PGSPVAPPAGATRR14 pKa = 11.84HH15 pKa = 5.3PRR17 pKa = 11.84RR18 pKa = 11.84HH19 pKa = 5.15GLAARR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84AMLRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84HH34 pKa = 4.62RR35 pKa = 11.84TGQRR39 pKa = 11.84HH40 pKa = 5.25PARR43 pKa = 11.84RR44 pKa = 11.84PRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84VRR50 pKa = 11.84PCRR53 pKa = 11.84ATARR57 pKa = 11.84PRR59 pKa = 3.23

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4050

0

4050

1347321

27

3333

332.7

35.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.791 ± 0.064

0.723 ± 0.01

6.948 ± 0.047

7.136 ± 0.047

2.584 ± 0.022

9.518 ± 0.039

2.377 ± 0.021

3.179 ± 0.024

1.159 ± 0.02

10.318 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.583 ± 0.016

1.439 ± 0.016

6.0 ± 0.032

2.694 ± 0.023

8.559 ± 0.052

4.587 ± 0.025

5.393 ± 0.024

8.888 ± 0.033

1.439 ± 0.016

1.684 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski