Rugamonas rubra

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Rugamonas

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5817 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4RUN0|A0A1I4RUN0_9BURK Thiazole synthase OS=Rugamonas rubra OX=758825 GN=thiG PE=3 SV=1
MM1 pKa = 7.15NAVAEE6 pKa = 4.42AQDD9 pKa = 4.47VIPSPIIFTDD19 pKa = 3.54SAAQKK24 pKa = 9.24VAQLIEE30 pKa = 4.37EE31 pKa = 4.51EE32 pKa = 4.63GNPDD36 pKa = 2.85LKK38 pKa = 11.13LRR40 pKa = 11.84VFVQGGGCSGFQYY53 pKa = 10.94GFTFDD58 pKa = 4.69EE59 pKa = 4.93IVNEE63 pKa = 5.02DD64 pKa = 4.09DD65 pKa = 2.98TTMIKK70 pKa = 10.62NGVQLLIDD78 pKa = 3.77SMSYY82 pKa = 10.23QYY84 pKa = 11.55LVGAEE89 pKa = 4.01IDD91 pKa = 3.89YY92 pKa = 11.28KK93 pKa = 11.39DD94 pKa = 4.47DD95 pKa = 4.08LEE97 pKa = 4.56GAQFVIKK104 pKa = 10.65NPTASSTCGCGSSFSVV120 pKa = 3.54

Molecular weight: 12.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4REW2|A0A1I4REW2_9BURK D-amino acid dehydrogenase OS=Rugamonas rubra OX=758825 GN=dadA PE=3 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84KK3 pKa = 9.74DD4 pKa = 3.29EE5 pKa = 4.18QQRR8 pKa = 11.84QEE10 pKa = 3.82PKK12 pKa = 9.0GQYY15 pKa = 8.67RR16 pKa = 11.84VKK18 pKa = 10.62NWPAYY23 pKa = 9.2NAGLIARR30 pKa = 11.84GDD32 pKa = 3.38VTIWIEE38 pKa = 3.52RR39 pKa = 11.84DD40 pKa = 2.62LWQRR44 pKa = 11.84GAEE47 pKa = 3.98AVKK50 pKa = 10.05RR51 pKa = 11.84GRR53 pKa = 11.84PRR55 pKa = 11.84VYY57 pKa = 10.55ADD59 pKa = 4.31AVIQMLLGLKK69 pKa = 9.68QVFRR73 pKa = 11.84LPLRR77 pKa = 11.84AAGFRR82 pKa = 11.84RR83 pKa = 11.84EE84 pKa = 4.2HH85 pKa = 5.87EE86 pKa = 4.29QACLRR91 pKa = 11.84RR92 pKa = 11.84SAGAQLHH99 pKa = 6.62DD100 pKa = 4.29AVSPRR105 pKa = 11.84PGSRR109 pKa = 11.84RR110 pKa = 11.84RR111 pKa = 11.84AADD114 pKa = 3.15PAQRR118 pKa = 11.84RR119 pKa = 11.84TVASRR124 pKa = 11.84GRR126 pKa = 11.84QHH128 pKa = 7.22RR129 pKa = 11.84PDD131 pKa = 3.65LVATITSCSGGRR143 pKa = 11.84ATSSCASCTALAAGRR158 pKa = 11.84SHH160 pKa = 6.92SCWQRR165 pKa = 11.84SWCTDD170 pKa = 2.63RR171 pKa = 11.84RR172 pKa = 11.84GRR174 pKa = 11.84PCLDD178 pKa = 3.1CRR180 pKa = 11.84QSFSAWAATKK190 pKa = 10.09LASSIMGSGSMPWLALSAVALVTLAAGAQYY220 pKa = 11.09LEE222 pKa = 4.33RR223 pKa = 11.84TEE225 pKa = 4.18DD226 pKa = 3.33AAEE229 pKa = 4.18IGVKK233 pKa = 10.05HH234 pKa = 6.32LPCCKK239 pKa = 9.22YY240 pKa = 9.28TNTFTNVVNKK250 pKa = 10.34CKK252 pKa = 10.65

Molecular weight: 27.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5817

0

5817

2007833

39

3183

345.2

37.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.829 ± 0.058

0.894 ± 0.011

5.254 ± 0.021

5.027 ± 0.028

3.456 ± 0.02

8.384 ± 0.037

2.146 ± 0.016

4.332 ± 0.025

3.457 ± 0.028

11.129 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.383 ± 0.014

2.941 ± 0.023

4.949 ± 0.028

4.148 ± 0.026

6.548 ± 0.033

5.561 ± 0.027

4.763 ± 0.032

7.066 ± 0.026

1.296 ± 0.013

2.437 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski