Candidatus Aquarickettsia rohweri

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Candidatus Midichloriaceae; Candidatus Aquarickettsia

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1222 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A429XSN2|A0A429XSN2_9RICK Flagellum-specific ATP synthase OS=Candidatus Aquarickettsia rohweri OX=2602574 GN=fliI PE=3 SV=1
MM1 pKa = 7.54SNVKK5 pKa = 10.25LNYY8 pKa = 9.59ILFDD12 pKa = 3.16QDD14 pKa = 4.45YY15 pKa = 10.97ILTNTNAKK23 pKa = 10.38NYY25 pKa = 10.62LLDD28 pKa = 4.59NDD30 pKa = 3.91NQLQISLPIIITQSEE45 pKa = 4.26VDD47 pKa = 3.6EE48 pKa = 4.42MAGKK52 pKa = 10.42VLDD55 pKa = 5.02DD56 pKa = 3.82FTTNDD61 pKa = 3.66IIVSLTLPITYY72 pKa = 10.62AFLKK76 pKa = 10.98VNDD79 pKa = 4.16LVQIEE84 pKa = 4.66YY85 pKa = 10.51EE86 pKa = 4.1DD87 pKa = 3.93QILILQIFQIIIDD100 pKa = 3.79EE101 pKa = 4.46TFTISIICEE110 pKa = 3.67RR111 pKa = 11.84ADD113 pKa = 3.15

Molecular weight: 13.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A429XGV8|A0A429XGV8_9RICK DNA gyrase subunit A OS=Candidatus Aquarickettsia rohweri OX=2602574 GN=gyrA PE=3 SV=1
MM1 pKa = 6.23STKK4 pKa = 9.68RR5 pKa = 11.84TFQPSNIKK13 pKa = 9.92RR14 pKa = 11.84KK15 pKa = 8.84RR16 pKa = 11.84RR17 pKa = 11.84HH18 pKa = 4.71GFRR21 pKa = 11.84SRR23 pKa = 11.84MATVGGRR30 pKa = 11.84KK31 pKa = 9.1IINSRR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 9.72GRR41 pKa = 11.84KK42 pKa = 8.69ILSAA46 pKa = 4.02

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1222

0

1222

356515

28

1580

291.7

33.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.09 ± 0.062

1.132 ± 0.027

5.331 ± 0.05

6.031 ± 0.072

5.136 ± 0.073

5.281 ± 0.069

1.675 ± 0.028

10.909 ± 0.083

10.021 ± 0.084

9.629 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.987 ± 0.027

7.48 ± 0.076

2.757 ± 0.038

2.99 ± 0.042

2.859 ± 0.043

7.193 ± 0.045

4.443 ± 0.038

5.201 ± 0.051

0.746 ± 0.026

4.11 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski