Mesorhizobium ciceri biovar biserrulae (strain HAMBI 2942 / LMG 23838 / WSM1271)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium; Mesorhizobium ciceri; Mesorhizobium ciceri biovar biserrulae

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6260 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E8TH78|E8TH78_MESCW Cobaltochelatase CobN subunit OS=Mesorhizobium ciceri biovar biserrulae (strain HAMBI 2942 / LMG 23838 / WSM1271) OX=765698 GN=Mesci_3280 PE=4 SV=1
MM1 pKa = 7.72LDD3 pKa = 3.07ITAQDD8 pKa = 3.63IIIDD12 pKa = 4.1EE13 pKa = 4.52TTGLQDD19 pKa = 3.26SDD21 pKa = 4.41VNPTVPPHH29 pKa = 6.25NNATVSYY36 pKa = 10.23LLGLDD41 pKa = 3.75GPGGLTSPEE50 pKa = 3.9VAFKK54 pKa = 11.4SNFVVASASAGEE66 pKa = 4.49TITSVVLTQNASGTPFSTTVGVNSGIRR93 pKa = 11.84TVDD96 pKa = 3.25GNYY99 pKa = 9.45VWLFKK104 pKa = 10.81DD105 pKa = 3.18ATHH108 pKa = 6.7ANVVIGVIGTSNPLVAPAATGPLAYY133 pKa = 10.17SFALITTDD141 pKa = 2.91ATHH144 pKa = 7.11ADD146 pKa = 4.67LYY148 pKa = 8.92TVQYY152 pKa = 10.85VPLLHH157 pKa = 6.94PVATDD162 pKa = 3.35PDD164 pKa = 3.97DD165 pKa = 6.32RR166 pKa = 11.84IDD168 pKa = 3.72LTDD171 pKa = 3.55HH172 pKa = 6.13VFASVSGTTVANFSGQNAAPGNHH195 pKa = 6.94DD196 pKa = 4.47FYY198 pKa = 11.36AINSSGGAASQLLVTGFLGANNATANVSTQGFGVNNQSINPTEE241 pKa = 3.96TLQVDD246 pKa = 4.99FVSGANLPAGSASQIQYY263 pKa = 10.54GSHH266 pKa = 6.78IDD268 pKa = 3.98NITHH272 pKa = 7.35AGFTINQITPSNPNLRR288 pKa = 11.84VDD290 pKa = 4.0IKK292 pKa = 10.25ITALDD297 pKa = 3.59VQGNEE302 pKa = 3.95QGLNFYY308 pKa = 10.71DD309 pKa = 4.94GSPTTAAPITSLTLIGQSGVASPITANGTYY339 pKa = 10.43DD340 pKa = 3.13VAGNVDD346 pKa = 3.61VTISGLGTNIVTITGLDD363 pKa = 3.49NITTVNVTTSSQMDD377 pKa = 3.73RR378 pKa = 11.84LLVTGVDD385 pKa = 3.83SNEE388 pKa = 4.03GCDD391 pKa = 2.79ITEE394 pKa = 4.15FHH396 pKa = 7.31FSTTTTNAYY405 pKa = 8.33TEE407 pKa = 4.27QVGSFINFDD416 pKa = 3.77DD417 pKa = 5.43DD418 pKa = 5.13GPTLSITAAPVVGAAEE434 pKa = 4.31VIEE437 pKa = 4.04ASGAGGHH444 pKa = 5.49SQATITPPTFTASAVDD460 pKa = 3.96GVTTNVTYY468 pKa = 11.18ALALAGGAATGLLTTEE484 pKa = 4.34GNHH487 pKa = 7.34AITLVVDD494 pKa = 4.07SANQISGQYY503 pKa = 10.58DD504 pKa = 3.29SDD506 pKa = 3.93GDD508 pKa = 4.23SVLDD512 pKa = 3.47ATAFTVTLSGTTVTLTSLVALEE534 pKa = 4.58HH535 pKa = 7.03SNTQGVGEE543 pKa = 5.13DD544 pKa = 3.35NTLDD548 pKa = 3.78LNGLINVVATVTATDD563 pKa = 3.54GDD565 pKa = 4.04NDD567 pKa = 3.85VVSQQSSSSGLSLTFDD583 pKa = 3.84DD584 pKa = 4.76TDD586 pKa = 3.6PTLSITAATVVGAAEE601 pKa = 4.17VVEE604 pKa = 4.03ASGAGGHH611 pKa = 5.49SQATITPPTFTASAVDD627 pKa = 3.96GVTTNVTYY635 pKa = 11.18ALALAGGAATGLLTTEE651 pKa = 4.34GNHH654 pKa = 7.33AITLVADD661 pKa = 3.84SANQISGQYY670 pKa = 10.58DD671 pKa = 3.29SDD673 pKa = 3.93GDD675 pKa = 4.23SVLDD679 pKa = 3.47ATAFTVTLSGTTVTLTSLVALEE701 pKa = 5.01HH702 pKa = 6.78SNAPQGVGEE711 pKa = 5.5DD712 pKa = 3.39NTLDD716 pKa = 3.78LNGLINVVATVTATDD731 pKa = 3.54GDD733 pKa = 4.04NDD735 pKa = 3.87VVSQQSTSSGLSLTFDD751 pKa = 3.84DD752 pKa = 4.76TDD754 pKa = 3.6PTLSITAATVVGAAGVIEE772 pKa = 3.97ASGVGGQATITPPAFTASAVDD793 pKa = 3.93GVTTNVTYY801 pKa = 11.18ALALAGGAATGLLTTEE817 pKa = 4.34GNHH820 pKa = 7.34AITLVVDD827 pKa = 3.94SATQVSGQYY836 pKa = 10.77DD837 pKa = 3.51SDD839 pKa = 3.93GDD841 pKa = 4.23SVLDD845 pKa = 3.47ATAFTVTLSGTTVTLTSLVALEE867 pKa = 4.58HH868 pKa = 7.03SNTQGVGEE876 pKa = 5.13DD877 pKa = 3.35NTLDD881 pKa = 3.78LNGLINVVATVTATDD896 pKa = 3.54GDD898 pKa = 4.04NDD900 pKa = 3.85VVSQQSSSSGPLSLTFDD917 pKa = 3.96DD918 pKa = 4.45TDD920 pKa = 3.62PTITVPFDD928 pKa = 3.56GDD930 pKa = 3.42QNAGNGTGTHH940 pKa = 4.98EE941 pKa = 4.26TLANTLNASAIGAFGYY957 pKa = 11.26DD958 pKa = 3.09MVDD961 pKa = 2.53KK962 pKa = 9.75HH963 pKa = 5.59TAAEE967 pKa = 4.14YY968 pKa = 10.58LAGASDD974 pKa = 3.92FVDD977 pKa = 3.41VNGALAGIQITLDD990 pKa = 3.48GNLTGLVPAPGTTPFLSSVATLQSEE1015 pKa = 4.82SATSATFNWTASYY1028 pKa = 11.32DD1029 pKa = 3.9SDD1031 pKa = 4.51PNTAGIQPGSVGGTLVFHH1049 pKa = 7.35KK1050 pKa = 10.77DD1051 pKa = 2.65AGTYY1055 pKa = 9.25TITLSDD1061 pKa = 3.61TVEE1064 pKa = 4.52GFTKK1068 pKa = 10.64DD1069 pKa = 3.0ILHH1072 pKa = 6.12TSEE1075 pKa = 5.62LLFKK1079 pKa = 10.7EE1080 pKa = 4.13PLSNTGHH1087 pKa = 6.65PNIVVEE1093 pKa = 4.94KK1094 pKa = 10.4LFEE1097 pKa = 5.22ADD1099 pKa = 3.63STPEE1103 pKa = 3.69TTDD1106 pKa = 2.76RR1107 pKa = 11.84DD1108 pKa = 3.85FFVQFTGNSNPNGSPLGFNATGDD1131 pKa = 3.87GAPAGLPNNLDD1142 pKa = 3.5TAFDD1146 pKa = 3.94AGQQISSNFEE1156 pKa = 3.58DD1157 pKa = 4.59WISATQATNGVAGDD1171 pKa = 4.49TIQKK1175 pKa = 10.86GEE1177 pKa = 4.31LLTLRR1182 pKa = 11.84FFDD1185 pKa = 3.95HH1186 pKa = 6.85SPGIVTDD1193 pKa = 4.04DD1194 pKa = 3.63GVNNVPNQSATDD1206 pKa = 3.61MAIKK1210 pKa = 10.25FDD1212 pKa = 4.67GIGNSEE1218 pKa = 4.65DD1219 pKa = 4.2LMLILNLVNYY1229 pKa = 9.79GSDD1232 pKa = 4.09GIAGGSGTAADD1243 pKa = 4.1TFTTKK1248 pKa = 10.65AMYY1251 pKa = 10.34VSNTNIFKK1259 pKa = 10.76AGQVPTAYY1267 pKa = 10.37AADD1270 pKa = 4.05FPLDD1274 pKa = 3.81NNDD1277 pKa = 3.21GLVIIEE1283 pKa = 4.36KK1284 pKa = 10.26NDD1286 pKa = 3.36YY1287 pKa = 10.47NATGEE1292 pKa = 4.0NWVLQGAQIMQSGNGLTGTAIDD1314 pKa = 4.32LNKK1317 pKa = 9.96ATGTGTTGASTGTHH1331 pKa = 6.11AFDD1334 pKa = 3.57VTDD1337 pKa = 3.78NDD1339 pKa = 3.58VLKK1342 pKa = 9.79ITDD1345 pKa = 3.34IGFTSTQTTTPDD1357 pKa = 2.8AHH1359 pKa = 7.95LDD1361 pKa = 3.53FAFQVADD1368 pKa = 3.59ADD1370 pKa = 4.06GDD1372 pKa = 4.13VTVPQHH1378 pKa = 6.18ILVDD1382 pKa = 4.0VVV1384 pKa = 3.19

Molecular weight: 140.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E8TDS6|E8TDS6_MESCW Sarcosine oxidase delta subunit family protein OS=Mesorhizobium ciceri biovar biserrulae (strain HAMBI 2942 / LMG 23838 / WSM1271) OX=765698 GN=Mesci_1815 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.11GGRR28 pKa = 11.84GVVAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6260

0

6260

1921430

30

3582

306.9

33.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.319 ± 0.042

0.823 ± 0.01

5.709 ± 0.023

5.405 ± 0.03

3.906 ± 0.02

8.706 ± 0.047

2.017 ± 0.014

5.486 ± 0.021

3.703 ± 0.026

9.859 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.014

2.751 ± 0.019

5.015 ± 0.023

3.058 ± 0.018

6.658 ± 0.038

5.658 ± 0.021

5.35 ± 0.028

7.463 ± 0.024

1.337 ± 0.014

2.246 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski