Aliiroseovarius crassostreae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Aliiroseovarius

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3465 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P7ITD1|A0A0P7ITD1_9RHOB L-aspartate oxidase OS=Aliiroseovarius crassostreae OX=154981 GN=AKJ29_07290 PE=3 SV=1
MM1 pKa = 6.98ATGYY5 pKa = 10.22LVQLGDD11 pKa = 3.71SSLDD15 pKa = 3.4SGDD18 pKa = 4.96AIIGPTTSFTIDD30 pKa = 3.48TILGSGSWTWSGTYY44 pKa = 10.58GGTTYY49 pKa = 10.83TNEE52 pKa = 4.38TEE54 pKa = 3.85TGTYY58 pKa = 10.6YY59 pKa = 10.88LGSDD63 pKa = 3.34GNIYY67 pKa = 8.37FTSNNGPVDD76 pKa = 4.39TISSASVISAPSYY89 pKa = 9.53STADD93 pKa = 3.55DD94 pKa = 3.87VLTGGDD100 pKa = 3.69GADD103 pKa = 3.91VIDD106 pKa = 4.36TSFTDD111 pKa = 3.53EE112 pKa = 5.28DD113 pKa = 4.36GEE115 pKa = 4.65KK116 pKa = 10.58VDD118 pKa = 4.95GGDD121 pKa = 4.22GATGDD126 pKa = 3.73NADD129 pKa = 4.08VVDD132 pKa = 5.39AGAGDD137 pKa = 4.64DD138 pKa = 4.02YY139 pKa = 12.1VEE141 pKa = 4.83AGYY144 pKa = 11.36GDD146 pKa = 3.6DD147 pKa = 4.21TIYY150 pKa = 11.2GGTGSDD156 pKa = 3.77TIEE159 pKa = 4.65GGDD162 pKa = 4.18GNDD165 pKa = 4.69SIYY168 pKa = 11.18GDD170 pKa = 4.89DD171 pKa = 4.8DD172 pKa = 4.67LSSVTPEE179 pKa = 4.95PITIHH184 pKa = 6.3EE185 pKa = 4.83GNFTDD190 pKa = 3.59TTNGYY195 pKa = 7.54TVTAQNVVGGSLTTASSSNIATYY218 pKa = 10.71SGGGFGASGTVSDD231 pKa = 4.75SDD233 pKa = 4.2SSVTSQIGYY242 pKa = 10.02DD243 pKa = 3.59LASGLSEE250 pKa = 4.08NVTVTLDD257 pKa = 3.76GEE259 pKa = 4.09ASEE262 pKa = 5.45ASFSFANLYY271 pKa = 9.2TSTYY275 pKa = 10.98AEE277 pKa = 4.23AGHH280 pKa = 5.78WAIYY284 pKa = 10.21NDD286 pKa = 3.54GVLVAEE292 pKa = 5.26GDD294 pKa = 3.82FTEE297 pKa = 4.57QGAGTGSGTVDD308 pKa = 2.89ISGHH312 pKa = 5.38GNFDD316 pKa = 3.7QIVFTALPQTDD327 pKa = 3.7GTDD330 pKa = 3.25GSDD333 pKa = 3.42YY334 pKa = 11.01HH335 pKa = 6.05LTSVSFTPVPVDD347 pKa = 4.39PIAGNDD353 pKa = 3.78SLSGGAGDD361 pKa = 6.18DD362 pKa = 4.4FIDD365 pKa = 5.03GNGGDD370 pKa = 4.03DD371 pKa = 3.75TLTGGSGSDD380 pKa = 3.43TLHH383 pKa = 6.7GGDD386 pKa = 4.58GEE388 pKa = 4.58DD389 pKa = 4.87LIHH392 pKa = 7.06VGAGDD397 pKa = 3.72TASGGTGSDD406 pKa = 2.88RR407 pKa = 11.84FEE409 pKa = 5.45LNPTDD414 pKa = 4.71ALDD417 pKa = 4.13GSGPTITLDD426 pKa = 3.38GGEE429 pKa = 4.91DD430 pKa = 3.95ADD432 pKa = 6.08DD433 pKa = 4.44SDD435 pKa = 6.11IDD437 pKa = 3.93TLDD440 pKa = 3.81LKK442 pKa = 11.41GLVNDD447 pKa = 4.26WSNVVFDD454 pKa = 5.27PDD456 pKa = 3.46NPEE459 pKa = 4.07NGTATLSDD467 pKa = 3.59GTVVTFTNFEE477 pKa = 4.03QIIICFTGSTRR488 pKa = 11.84IEE490 pKa = 4.4TPFGPRR496 pKa = 11.84PVQDD500 pKa = 4.46LRR502 pKa = 11.84PGDD505 pKa = 4.59LIITRR510 pKa = 11.84DD511 pKa = 3.51NGLQPLLWIGKK522 pKa = 7.44SHH524 pKa = 6.65VAAQGRR530 pKa = 11.84LAPIRR535 pKa = 11.84FCKK538 pKa = 9.99GAIGNSRR545 pKa = 11.84PLLVSPQHH553 pKa = 6.77RR554 pKa = 11.84ILLKK558 pKa = 10.54SPKK561 pKa = 9.16ATLLFDD567 pKa = 3.74TPEE570 pKa = 4.04VLVPACHH577 pKa = 6.52MINGEE582 pKa = 4.21TIYY585 pKa = 10.78RR586 pKa = 11.84EE587 pKa = 3.97AASEE591 pKa = 3.93VTYY594 pKa = 10.79YY595 pKa = 10.98HH596 pKa = 7.17LLFDD600 pKa = 3.63QHH602 pKa = 5.85EE603 pKa = 4.68VIYY606 pKa = 10.88SEE608 pKa = 4.42GVPTEE613 pKa = 4.29SFHH616 pKa = 6.8PAWPTLGGLDD626 pKa = 3.36TQTRR630 pKa = 11.84EE631 pKa = 3.74EE632 pKa = 4.18VLALFPALRR641 pKa = 11.84CSNTGYY647 pKa = 11.05GKK649 pKa = 7.71TARR652 pKa = 11.84SVLRR656 pKa = 11.84RR657 pKa = 11.84YY658 pKa = 7.42EE659 pKa = 4.05TRR661 pKa = 11.84LLLAGG666 pKa = 4.15

Molecular weight: 68.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P7JRR9|A0A0P7JRR9_9RHOB MPT synthase subunit 2 OS=Aliiroseovarius crassostreae OX=154981 GN=AKJ29_15895 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.45AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3465

0

3465

1035234

38

2637

298.8

32.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.435 ± 0.053

0.894 ± 0.014

6.069 ± 0.036

6.174 ± 0.037

3.815 ± 0.025

8.552 ± 0.044

2.109 ± 0.022

5.297 ± 0.028

3.778 ± 0.035

9.959 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.849 ± 0.021

2.856 ± 0.03

4.884 ± 0.031

3.393 ± 0.024

6.423 ± 0.041

5.36 ± 0.031

5.383 ± 0.028

7.142 ± 0.035

1.359 ± 0.017

2.267 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski