Ralstonia phage RSP15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; unclassified Ackermannviridae

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 244 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A125STA6|A0A125STA6_9CAUD Uncharacterized protein OS=Ralstonia phage RSP15 OX=1785960 PE=4 SV=1
MM1 pKa = 7.58GKK3 pKa = 9.52LRR5 pKa = 11.84NQTYY9 pKa = 9.26QVVDD13 pKa = 3.33NGEE16 pKa = 3.97GKK18 pKa = 9.76VAYY21 pKa = 9.58VQIISGHH28 pKa = 4.22YY29 pKa = 8.0TGLTVSYY36 pKa = 10.39GGISFSEE43 pKa = 4.34QEE45 pKa = 4.6DD46 pKa = 4.5GATLHH51 pKa = 6.78FDD53 pKa = 3.42YY54 pKa = 11.05DD55 pKa = 3.96IVSNPYY61 pKa = 9.62NVPIDD66 pKa = 4.53DD67 pKa = 3.68EE68 pKa = 4.42TLRR71 pKa = 11.84PVLGDD76 pKa = 3.74CLVDD80 pKa = 5.45IIDD83 pKa = 4.9DD84 pKa = 4.03LMKK87 pKa = 10.74KK88 pKa = 8.61EE89 pKa = 3.94

Molecular weight: 9.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0X8WQF8|A0A0X8WQF8_9CAUD Uncharacterized protein OS=Ralstonia phage RSP15 OX=1785960 PE=4 SV=1
MM1 pKa = 7.92LDD3 pKa = 4.52FDD5 pKa = 5.69DD6 pKa = 5.47FLNLAEE12 pKa = 4.24AFKK15 pKa = 10.97FKK17 pKa = 10.91VKK19 pKa = 9.55STGQKK24 pKa = 8.69RR25 pKa = 11.84KK26 pKa = 10.2KK27 pKa = 10.07RR28 pKa = 11.84ICPPGYY34 pKa = 9.82KK35 pKa = 9.65PSPDD39 pKa = 3.35GSSCVPMRR47 pKa = 11.84PSEE50 pKa = 4.01KK51 pKa = 9.68RR52 pKa = 11.84SRR54 pKa = 11.84KK55 pKa = 9.48LGAKK59 pKa = 9.81RR60 pKa = 11.84GVRR63 pKa = 11.84DD64 pKa = 4.22KK65 pKa = 10.78KK66 pKa = 11.1AQGAGLKK73 pKa = 10.22NRR75 pKa = 11.84TKK77 pKa = 10.79RR78 pKa = 11.84KK79 pKa = 6.49TKK81 pKa = 9.98KK82 pKa = 9.46AMRR85 pKa = 11.84YY86 pKa = 9.36RR87 pKa = 11.84KK88 pKa = 8.91MFGLL92 pKa = 4.01

Molecular weight: 10.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

244

0

244

49577

51

1615

203.2

22.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.34 ± 0.218

0.964 ± 0.065

6.461 ± 0.128

6.66 ± 0.252

4.71 ± 0.107

7.056 ± 0.199

1.715 ± 0.095

6.287 ± 0.122

7.088 ± 0.239

7.389 ± 0.147

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.743 ± 0.09

5.531 ± 0.157

3.752 ± 0.095

3.59 ± 0.101

4.377 ± 0.139

5.948 ± 0.162

5.904 ± 0.21

6.88 ± 0.159

1.386 ± 0.066

4.22 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski