Escherichia phage PA28

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Sepvirinae; Traversvirus; Escherichia virus PA28

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N7BTP4|A0A0N7BTP4_9CAUD Uncharacterized protein OS=Escherichia phage PA28 OX=1660368 PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 10.33LRR4 pKa = 11.84VWHH7 pKa = 6.62IPQVPMKK14 pKa = 9.37PFIVEE19 pKa = 4.0VTSVEE24 pKa = 4.0EE25 pKa = 4.5GVRR28 pKa = 11.84LMDD31 pKa = 5.18ALADD35 pKa = 3.81YY36 pKa = 11.01DD37 pKa = 4.43AFQYY41 pKa = 11.41DD42 pKa = 4.1NNIKK46 pKa = 10.06PDD48 pKa = 3.47YY49 pKa = 10.48CNANGLEE56 pKa = 4.09MWDD59 pKa = 3.92EE60 pKa = 4.23SLTDD64 pKa = 3.56EE65 pKa = 5.26DD66 pKa = 4.5LSEE69 pKa = 4.37MGLTDD74 pKa = 4.8RR75 pKa = 11.84WVDD78 pKa = 3.24WYY80 pKa = 10.98SEE82 pKa = 4.2CQCYY86 pKa = 10.78DD87 pKa = 3.35DD88 pKa = 3.75ARR90 pKa = 11.84KK91 pKa = 9.93YY92 pKa = 10.89LEE94 pKa = 4.02SLKK97 pKa = 10.82EE98 pKa = 3.9EE99 pKa = 4.1TSAAA103 pKa = 3.68

Molecular weight: 12.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N7BZQ3|A0A0N7BZQ3_9CAUD Protein ninH OS=Escherichia phage PA28 OX=1660368 PE=3 SV=1
MM1 pKa = 6.97STIAEE6 pKa = 4.05LVRR9 pKa = 11.84ANFRR13 pKa = 11.84EE14 pKa = 4.1EE15 pKa = 3.58LVRR18 pKa = 11.84WYY20 pKa = 10.29RR21 pKa = 11.84YY22 pKa = 9.46RR23 pKa = 11.84SSSSLPLDD31 pKa = 3.41EE32 pKa = 6.07LYY34 pKa = 10.73EE35 pKa = 4.16HH36 pKa = 6.91SPAARR41 pKa = 11.84RR42 pKa = 11.84YY43 pKa = 8.71PRR45 pKa = 11.84DD46 pKa = 3.04RR47 pKa = 11.84VLRR50 pKa = 11.84RR51 pKa = 11.84LFKK54 pKa = 11.01LNNEE58 pKa = 4.2FQRR61 pKa = 11.84NRR63 pKa = 11.84IIRR66 pKa = 11.84SLDD69 pKa = 3.3FKK71 pKa = 11.5

Molecular weight: 8.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

18092

37

2791

201.0

22.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.584 ± 0.368

1.177 ± 0.205

5.804 ± 0.221

6.837 ± 0.264

3.112 ± 0.167

7.213 ± 0.478

1.708 ± 0.134

4.964 ± 0.307

5.997 ± 0.249

7.793 ± 0.212

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.797 ± 0.146

4.306 ± 0.161

4.289 ± 0.252

4.577 ± 0.3

6.854 ± 0.267

6.152 ± 0.256

5.605 ± 0.222

6.456 ± 0.232

1.747 ± 0.164

3.029 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski