Vibrio phage vB_VchM_Kuja

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; Kujavirus; Vibrio virus Kuja

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 186 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9J7X2|A0A6B9J7X2_9CAUD Uncharacterized protein OS=Vibrio phage vB_VchM_Kuja OX=2686437 GN=Kuja_1630 PE=4 SV=1
MM1 pKa = 7.48SNEE4 pKa = 4.71GILITGDD11 pKa = 3.63NPQLNYY17 pKa = 10.74VLVSKK22 pKa = 8.31FTFSIPCSDD31 pKa = 3.51ATPIKK36 pKa = 10.78QMVEE40 pKa = 3.89EE41 pKa = 4.13VNKK44 pKa = 10.18INEE47 pKa = 4.17NIKK50 pKa = 10.77YY51 pKa = 10.51SFVSCDD57 pKa = 3.56DD58 pKa = 3.79NVDD61 pKa = 3.61EE62 pKa = 5.35GEE64 pKa = 4.12TAIGYY69 pKa = 9.13VEE71 pKa = 4.75VEE73 pKa = 4.17TTTPLLHH80 pKa = 6.65EE81 pKa = 5.17SISIIFDD88 pKa = 3.68CLYY91 pKa = 10.79KK92 pKa = 10.3ADD94 pKa = 4.24KK95 pKa = 10.92EE96 pKa = 4.26DD97 pKa = 5.85VKK99 pKa = 11.05DD100 pKa = 3.61HH101 pKa = 5.93WCDD104 pKa = 3.41YY105 pKa = 11.46VDD107 pKa = 3.57IEE109 pKa = 4.88SINVVSANYY118 pKa = 8.37DD119 pKa = 3.38TIYY122 pKa = 10.71PMVGGFVEE130 pKa = 4.65EE131 pKa = 4.28FTNFYY136 pKa = 10.86HH137 pKa = 6.36VFCRR141 pKa = 11.84LQDD144 pKa = 3.73AEE146 pKa = 4.23QTITDD151 pKa = 4.37LNEE154 pKa = 3.94ALNIQNEE161 pKa = 4.76TIGNLTTVIKK171 pKa = 10.27EE172 pKa = 4.07AVEE175 pKa = 3.78KK176 pKa = 10.43TEE178 pKa = 4.01NLKK181 pKa = 10.96SYY183 pKa = 10.62INQVLPNTGLSVDD196 pKa = 4.74DD197 pKa = 4.87LVTSNTIPLRR207 pKa = 11.84NEE209 pKa = 3.46

Molecular weight: 23.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9J5F6|A0A6B9J5F6_9CAUD Putative membrane protein OS=Vibrio phage vB_VchM_Kuja OX=2686437 GN=Kuja_1200 PE=4 SV=1
MM1 pKa = 7.71LEE3 pKa = 4.48HH4 pKa = 6.35IAKK7 pKa = 10.14RR8 pKa = 11.84FNANARR14 pKa = 11.84RR15 pKa = 11.84KK16 pKa = 9.6HH17 pKa = 4.72FLNRR21 pKa = 11.84IVNHH25 pKa = 5.58IEE27 pKa = 3.49KK28 pKa = 10.63GEE30 pKa = 3.87RR31 pKa = 11.84RR32 pKa = 11.84QALNVLEE39 pKa = 4.28KK40 pKa = 10.47GVRR43 pKa = 11.84GRR45 pKa = 11.84YY46 pKa = 6.08YY47 pKa = 9.93TGLPRR52 pKa = 11.84AEE54 pKa = 4.18RR55 pKa = 11.84PLILLCVWLVDD66 pKa = 3.29MGKK69 pKa = 9.53PEE71 pKa = 4.03KK72 pKa = 10.8ALVLIKK78 pKa = 10.84RR79 pKa = 11.84MVEE82 pKa = 3.97EE83 pKa = 4.24SS84 pKa = 3.1

Molecular weight: 9.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

186

0

186

46457

35

1568

249.8

28.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.657 ± 0.175

1.324 ± 0.088

6.027 ± 0.127

7.248 ± 0.189

4.682 ± 0.121

5.891 ± 0.144

1.866 ± 0.088

6.989 ± 0.152

7.284 ± 0.212

8.339 ± 0.127

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.602 ± 0.097

6.193 ± 0.148

3.401 ± 0.089

3.567 ± 0.098

4.529 ± 0.093

6.387 ± 0.14

5.747 ± 0.14

7.153 ± 0.14

1.145 ± 0.07

3.969 ± 0.132
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski