Human parainfluenza 2 virus (strain Toshiba) (HPIV-2)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Rubulavirinae; Orthorubulavirus; Human orthorubulavirus 2

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P23056|PHOSP_PI2HT Phosphoprotein OS=Human parainfluenza 2 virus (strain Toshiba) OX=11214 GN=P/V PE=3 SV=1
MM1 pKa = 7.45SSVLKK6 pKa = 9.72TFEE9 pKa = 4.27RR10 pKa = 11.84FTIQQEE16 pKa = 4.18LQEE19 pKa = 4.44QSDD22 pKa = 4.1DD23 pKa = 3.83TPVPLEE29 pKa = 4.27TIKK32 pKa = 9.66PTIRR36 pKa = 11.84VFVINNNDD44 pKa = 3.28PVVRR48 pKa = 11.84SRR50 pKa = 11.84LLFFNLRR57 pKa = 11.84IIMSNTARR65 pKa = 11.84EE66 pKa = 4.14GHH68 pKa = 6.37RR69 pKa = 11.84AGALLSLLSLPSAAMSNHH87 pKa = 6.13IKK89 pKa = 10.55LAMHH93 pKa = 6.37SPEE96 pKa = 3.82ASIDD100 pKa = 3.53RR101 pKa = 11.84VEE103 pKa = 3.97ITGFEE108 pKa = 4.17NNSFRR113 pKa = 11.84VIPDD117 pKa = 2.97ARR119 pKa = 11.84STMSRR124 pKa = 11.84GEE126 pKa = 3.95VLAFEE131 pKa = 5.18ALAEE135 pKa = 5.23DD136 pKa = 4.85IPDD139 pKa = 4.02TLNHH143 pKa = 5.17QTPFVNNDD151 pKa = 2.92VEE153 pKa = 5.65DD154 pKa = 5.53DD155 pKa = 3.81IFDD158 pKa = 3.73EE159 pKa = 4.62TEE161 pKa = 3.79KK162 pKa = 10.98FLDD165 pKa = 3.29VCYY168 pKa = 10.53SVLMQAWIVTCKK180 pKa = 10.52CMTAPDD186 pKa = 3.93QPPVSVAKK194 pKa = 8.98MAKK197 pKa = 8.5YY198 pKa = 9.12QQQGRR203 pKa = 11.84INARR207 pKa = 11.84YY208 pKa = 8.82VLQPEE213 pKa = 4.31AQRR216 pKa = 11.84LIQNAIRR223 pKa = 11.84KK224 pKa = 9.25SMVVRR229 pKa = 11.84HH230 pKa = 5.66FMTYY234 pKa = 9.61EE235 pKa = 3.85LQLSQSRR242 pKa = 11.84SLLANRR248 pKa = 11.84YY249 pKa = 8.02YY250 pKa = 11.74AMVGDD255 pKa = 3.33IGKK258 pKa = 9.94YY259 pKa = 9.26IEE261 pKa = 4.21HH262 pKa = 6.88SGMGGFFLTLKK273 pKa = 10.42YY274 pKa = 10.88GLGTRR279 pKa = 11.84WPTLALAAFSGEE291 pKa = 4.06LQKK294 pKa = 11.25LKK296 pKa = 11.14ALMLHH301 pKa = 5.45YY302 pKa = 10.47QSLGPMAKK310 pKa = 10.14YY311 pKa = 8.52MALLEE316 pKa = 4.47SPKK319 pKa = 10.97LMDD322 pKa = 4.6FVPSEE327 pKa = 4.17YY328 pKa = 10.32PLDD331 pKa = 3.47YY332 pKa = 10.53SYY334 pKa = 12.07AMGIGTVLDD343 pKa = 3.74TNMRR347 pKa = 11.84NYY349 pKa = 10.98AYY351 pKa = 10.24GRR353 pKa = 11.84SYY355 pKa = 11.47LNQQYY360 pKa = 8.84FQLGVEE366 pKa = 4.63TARR369 pKa = 11.84KK370 pKa = 7.79QQGAVDD376 pKa = 3.5NRR378 pKa = 11.84TAEE381 pKa = 4.24DD382 pKa = 3.74LGMTAADD389 pKa = 4.18KK390 pKa = 11.39ADD392 pKa = 3.54LTATISKK399 pKa = 10.37LSLSQLPRR407 pKa = 11.84GRR409 pKa = 11.84QPISDD414 pKa = 4.38PFAGANDD421 pKa = 3.87RR422 pKa = 11.84EE423 pKa = 4.54MGGQANDD430 pKa = 3.53TPVYY434 pKa = 10.22NFNPIDD440 pKa = 3.35TRR442 pKa = 11.84RR443 pKa = 11.84YY444 pKa = 10.7DD445 pKa = 3.68NYY447 pKa = 11.32DD448 pKa = 2.77SDD450 pKa = 4.84GEE452 pKa = 4.09DD453 pKa = 5.21RR454 pKa = 11.84IDD456 pKa = 3.8NDD458 pKa = 2.44QDD460 pKa = 3.19QAIRR464 pKa = 11.84EE465 pKa = 4.17NRR467 pKa = 11.84GEE469 pKa = 3.96PGQPNNQTSDD479 pKa = 2.88NQQRR483 pKa = 11.84FNPPIPQRR491 pKa = 11.84TSGMSSEE498 pKa = 4.47EE499 pKa = 4.09FQHH502 pKa = 7.27SMNQYY507 pKa = 8.22IRR509 pKa = 11.84AMHH512 pKa = 5.25EE513 pKa = 4.07QYY515 pKa = 10.5RR516 pKa = 11.84GSQDD520 pKa = 4.47DD521 pKa = 4.19DD522 pKa = 5.77ANDD525 pKa = 3.63ATDD528 pKa = 4.3GNDD531 pKa = 2.89ISLEE535 pKa = 4.02LVGDD539 pKa = 4.32FDD541 pKa = 4.53SS542 pKa = 4.56

Molecular weight: 61.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P25466|HN_PI2HT Hemagglutinin-neuraminidase OS=Human parainfluenza 2 virus (strain Toshiba) OX=11214 GN=HN PE=2 SV=1
MM1 pKa = 7.89PIISLPADD9 pKa = 3.61PTSPSQSLTPFPIQLDD25 pKa = 4.04TKK27 pKa = 10.51DD28 pKa = 3.56GKK30 pKa = 10.62AGKK33 pKa = 9.25LLKK36 pKa = 10.12QIRR39 pKa = 11.84IRR41 pKa = 11.84YY42 pKa = 8.65LNEE45 pKa = 3.71PNSRR49 pKa = 11.84HH50 pKa = 5.11TPITFINTYY59 pKa = 9.03GFVYY63 pKa = 10.59ARR65 pKa = 11.84DD66 pKa = 3.65TSGGIHH72 pKa = 6.18SEE74 pKa = 3.78ISSDD78 pKa = 3.47LAAGSITACMMKK90 pKa = 10.34LGPGPNIQNANLVLRR105 pKa = 11.84SLNEE109 pKa = 3.94FYY111 pKa = 11.5VKK113 pKa = 10.25VKK115 pKa = 9.13KK116 pKa = 9.1TSSQRR121 pKa = 11.84EE122 pKa = 3.82EE123 pKa = 4.0AVFEE127 pKa = 4.18LVNIPTLLRR136 pKa = 11.84EE137 pKa = 3.93HH138 pKa = 7.01ALCKK142 pKa = 10.28RR143 pKa = 11.84KK144 pKa = 8.9MLVCSAEE151 pKa = 4.19KK152 pKa = 10.24FLKK155 pKa = 10.77NPSKK159 pKa = 10.58LQAGFEE165 pKa = 4.24YY166 pKa = 10.76VYY168 pKa = 10.54IPTFVSITYY177 pKa = 10.1SPRR180 pKa = 11.84NLNYY184 pKa = 9.83QVARR188 pKa = 11.84PILKK192 pKa = 8.9FRR194 pKa = 11.84SRR196 pKa = 11.84FVYY199 pKa = 10.36SIHH202 pKa = 7.32LEE204 pKa = 4.3LILRR208 pKa = 11.84LLCKK212 pKa = 10.1SDD214 pKa = 3.63SPLMKK219 pKa = 10.11SYY221 pKa = 10.94NADD224 pKa = 3.52RR225 pKa = 11.84TGRR228 pKa = 11.84GCLASVWIHH237 pKa = 5.23VCNILKK243 pKa = 10.41NKK245 pKa = 10.02SIKK248 pKa = 8.63QQGRR252 pKa = 11.84EE253 pKa = 3.84SYY255 pKa = 10.7FIAKK259 pKa = 8.94CMSMQLQVSIADD271 pKa = 3.39LWGPTIIIKK280 pKa = 10.54SLGHH284 pKa = 5.95IPKK287 pKa = 8.96TALPFFSKK295 pKa = 10.69DD296 pKa = 3.19GIACHH301 pKa = 6.54PLQDD305 pKa = 4.33VSPNLAKK312 pKa = 10.67SLWSVGCEE320 pKa = 3.48IEE322 pKa = 4.31SAKK325 pKa = 10.85LILQEE330 pKa = 4.16SDD332 pKa = 3.5LNEE335 pKa = 4.22LMGHH339 pKa = 6.05QDD341 pKa = 5.06LITDD345 pKa = 4.79KK346 pKa = 10.78IAIRR350 pKa = 11.84SGQRR354 pKa = 11.84TFEE357 pKa = 3.96RR358 pKa = 11.84SKK360 pKa = 10.54FSPFKK365 pKa = 10.6KK366 pKa = 10.1YY367 pKa = 11.09ASIPNLEE374 pKa = 4.75AINN377 pKa = 3.66

Molecular weight: 42.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4923

225

2262

703.3

78.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.805 ± 0.646

1.747 ± 0.296

4.997 ± 0.508

4.591 ± 0.492

3.758 ± 0.252

5.2 ± 0.402

2.173 ± 0.228

8.694 ± 0.652

4.916 ± 0.475

10.42 ± 0.868

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.269

5.342 ± 0.513

5.322 ± 0.561

3.961 ± 0.371

4.672 ± 0.354

8.613 ± 0.244

6.967 ± 0.437

4.977 ± 0.349

0.975 ± 0.26

3.616 ± 0.456
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski