Desertifilum sp. IPPAS B-1220

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Desertifilaceae; Desertifilum; unclassified Desertifilum

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4964 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E5QFJ8|A0A1E5QFJ8_9CYAN Uncharacterized protein OS=Desertifilum sp. IPPAS B-1220 OX=1781255 GN=BH720_19675 PE=4 SV=1
MM1 pKa = 6.78IQEE4 pKa = 4.21WYY6 pKa = 9.31EE7 pKa = 3.87VWVDD11 pKa = 3.56EE12 pKa = 4.5STKK15 pKa = 10.11IPYY18 pKa = 10.1VLFLCPDD25 pKa = 4.11PNSSGGMLIIDD36 pKa = 3.88PKK38 pKa = 10.87EE39 pKa = 3.88NNQVIQNLPDD49 pKa = 3.51YY50 pKa = 8.91NTAILWLTEE59 pKa = 3.96DD60 pKa = 3.63EE61 pKa = 4.41YY62 pKa = 11.55TRR64 pKa = 11.84VEE66 pKa = 3.68GRR68 pKa = 11.84MEE70 pKa = 3.8IEE72 pKa = 3.88

Molecular weight: 8.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E5QJB2|A0A1E5QJB2_9CYAN Uncharacterized protein OS=Desertifilum sp. IPPAS B-1220 OX=1781255 GN=BH720_12945 PE=4 SV=1
MM1 pKa = 7.5LGFVPQPNLRR11 pKa = 11.84GEE13 pKa = 4.43LFFSQSGRR21 pKa = 11.84IFGIQDD27 pKa = 2.89VGFRR31 pKa = 11.84ASTQPTQRR39 pKa = 11.84AFFQSIRR46 pKa = 11.84AHH48 pKa = 6.12LWDD51 pKa = 3.48SRR53 pKa = 11.84CWVSCLNPTYY63 pKa = 10.37AASFFLVNQGAFLGFKK79 pKa = 9.65MLGFVPQPNLRR90 pKa = 11.84GEE92 pKa = 4.29LFSGNQRR99 pKa = 11.84TSLKK103 pKa = 10.4CKK105 pKa = 10.62SPLL108 pKa = 3.41

Molecular weight: 12.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4964

0

4964

1712571

31

2936

345.0

38.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.896 ± 0.035

0.983 ± 0.012

4.805 ± 0.03

6.401 ± 0.042

3.822 ± 0.021

6.814 ± 0.04

1.838 ± 0.016

6.18 ± 0.025

3.536 ± 0.031

11.489 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.752 ± 0.015

3.756 ± 0.028

5.303 ± 0.033

5.937 ± 0.031

5.796 ± 0.032

6.218 ± 0.027

5.574 ± 0.027

6.458 ± 0.029

1.504 ± 0.016

2.939 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski