Koolpinyah virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Ephemerovirus; Koolpinyah ephemerovirus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A096ZGT8|A0A096ZGT8_9RHAB GDP polyribonucleotidyltransferase OS=Koolpinyah virus OX=1550518 GN=L PE=4 SV=1
MM1 pKa = 7.45ALIMVEE7 pKa = 4.24GEE9 pKa = 4.31VGSSEE14 pKa = 4.18ASTGLVVTLVKK25 pKa = 10.27EE26 pKa = 4.41VNSLIMDD33 pKa = 4.38ILQCLDD39 pKa = 3.21IPFEE43 pKa = 4.52PGQMISRR50 pKa = 11.84KK51 pKa = 8.05DD52 pKa = 3.16TTMEE56 pKa = 3.37VDD58 pKa = 4.01FEE60 pKa = 4.49DD61 pKa = 5.87RR62 pKa = 11.84SVCFIKK68 pKa = 10.28IKK70 pKa = 10.72KK71 pKa = 7.46EE72 pKa = 3.82WEE74 pKa = 3.93VEE76 pKa = 4.33SIKK79 pKa = 11.11VGTLSFIEE87 pKa = 4.06EE88 pKa = 4.31LRR90 pKa = 11.84GKK92 pKa = 10.84GYY94 pKa = 10.6FSYY97 pKa = 10.58NGSMKK102 pKa = 10.48LDD104 pKa = 3.65ILPKK108 pKa = 9.88PP109 pKa = 3.83

Molecular weight: 12.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A096ZGT4|A0A096ZGT4_9RHAB Isoform of A0A096ZGT5 Alpha1 protein OS=Koolpinyah virus OX=1550518 GN=alpha PE=4 SV=1
MM1 pKa = 7.55SRR3 pKa = 11.84INWPSLDD10 pKa = 4.11GIKK13 pKa = 9.45TVLKK17 pKa = 9.8EE18 pKa = 4.1FPEE21 pKa = 4.75KK22 pKa = 10.61INTEE26 pKa = 3.62IKK28 pKa = 10.24EE29 pKa = 4.04FSHH32 pKa = 6.62NLVSKK37 pKa = 10.57IRR39 pKa = 11.84YY40 pKa = 6.32IWVWLVLIVLLLLLIKK56 pKa = 10.28LLPKK60 pKa = 9.82TINCIIGCKK69 pKa = 9.13RR70 pKa = 11.84CYY72 pKa = 11.0NNFSSGNKK80 pKa = 8.96RR81 pKa = 11.84LKK83 pKa = 10.26EE84 pKa = 4.15EE85 pKa = 4.27KK86 pKa = 10.56EE87 pKa = 3.88EE88 pKa = 4.19TAA90 pKa = 4.79

Molecular weight: 10.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

1

11

4786

90

2108

435.1

50.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.842 ± 0.451

1.922 ± 0.242

5.997 ± 0.502

7.355 ± 0.388

3.886 ± 0.302

5.955 ± 0.198

1.964 ± 0.2

9.089 ± 0.634

8.734 ± 0.382

9.444 ± 0.766

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.03 ± 0.313

6.435 ± 0.498

3.385 ± 0.217

2.403 ± 0.169

4.053 ± 0.284

7.188 ± 0.262

5.307 ± 0.332

4.973 ± 0.32

1.776 ± 0.196

4.262 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski