Flavonifractor sp. An135

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Flavonifractor; unclassified Flavonifractor

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3700 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4S0E8|A0A1Y4S0E8_9FIRM Recombinase family protein OS=Flavonifractor sp. An135 OX=1965558 GN=B5E80_10555 PE=4 SV=1
MM1 pKa = 7.77TIQALNGAKK10 pKa = 8.62EE11 pKa = 4.34TVDD14 pKa = 4.6LEE16 pKa = 4.59VPYY19 pKa = 10.46DD20 pKa = 3.69PQRR23 pKa = 11.84IAILDD28 pKa = 3.88MACLDD33 pKa = 3.67ILDD36 pKa = 4.34ALGVGDD42 pKa = 4.37RR43 pKa = 11.84VVGTADD49 pKa = 2.99TSLDD53 pKa = 3.63YY54 pKa = 11.33LQDD57 pKa = 4.05YY58 pKa = 10.93INDD61 pKa = 4.76DD62 pKa = 3.54IVNLGTIKK70 pKa = 10.56EE71 pKa = 4.15ADD73 pKa = 3.82LEE75 pKa = 4.21AVMACEE81 pKa = 4.41PDD83 pKa = 3.68VIFIGGRR90 pKa = 11.84LASSYY95 pKa = 11.12DD96 pKa = 3.75ALSEE100 pKa = 4.05IAPVVYY106 pKa = 10.58LSTDD110 pKa = 3.24TEE112 pKa = 4.59LGVVEE117 pKa = 4.98SVRR120 pKa = 11.84QNATTIASMFGLEE133 pKa = 4.38DD134 pKa = 3.64KK135 pKa = 11.04VDD137 pKa = 3.67EE138 pKa = 4.88LMSDD142 pKa = 3.69FDD144 pKa = 4.24ARR146 pKa = 11.84IAALAEE152 pKa = 3.94FAAGKK157 pKa = 7.21TAIVGMCTSGSYY169 pKa = 10.48NVLGNDD175 pKa = 3.97GRR177 pKa = 11.84CSLIGVEE184 pKa = 4.98VGFEE188 pKa = 4.23NIGVDD193 pKa = 3.81ANLDD197 pKa = 3.65TSTHH201 pKa = 5.36GNEE204 pKa = 3.91ASFEE208 pKa = 4.35FIVDD212 pKa = 3.82KK213 pKa = 11.26NPDD216 pKa = 3.83YY217 pKa = 11.02IFVMDD222 pKa = 4.67RR223 pKa = 11.84DD224 pKa = 3.83AAIGTDD230 pKa = 3.96GAQLAQDD237 pKa = 3.02IMEE240 pKa = 4.74NEE242 pKa = 4.11LVMGTNAYY250 pKa = 10.27QNGHH254 pKa = 6.18LVYY257 pKa = 10.15LAHH260 pKa = 6.72PAVWYY265 pKa = 7.69TAEE268 pKa = 4.27GGITALDD275 pKa = 4.12LMLQDD280 pKa = 5.65LEE282 pKa = 4.86SEE284 pKa = 4.43LLGG287 pKa = 4.23

Molecular weight: 30.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4RYG8|A0A1Y4RYG8_9FIRM Aminotransferase OS=Flavonifractor sp. An135 OX=1965558 GN=B5E80_14270 PE=3 SV=1
MM1 pKa = 7.06VRR3 pKa = 11.84TYY5 pKa = 10.22QPKK8 pKa = 9.15KK9 pKa = 7.59RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.92GFRR19 pKa = 11.84KK20 pKa = 10.08RR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 8.93VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.05GRR39 pKa = 11.84ARR41 pKa = 11.84LSHH44 pKa = 6.55

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3700

0

3700

1150233

25

2902

310.9

34.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.673 ± 0.053

1.628 ± 0.018

5.661 ± 0.033

7.078 ± 0.045

3.665 ± 0.026

7.959 ± 0.037

1.864 ± 0.019

5.065 ± 0.038

4.349 ± 0.04

10.404 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.583 ± 0.02

3.058 ± 0.027

4.446 ± 0.026

3.584 ± 0.023

5.95 ± 0.051

5.415 ± 0.03

5.703 ± 0.043

7.261 ± 0.037

1.206 ± 0.017

3.447 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski