Gordonia phage NHagos

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sourvirus; unclassified Sourvirus

Average proteome isoelectric point is 5.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WNH9|A0A5Q2WNH9_9CAUD Membrane protein OS=Gordonia phage NHagos OX=2653746 GN=81 PE=4 SV=1
MM1 pKa = 7.53PTTEE5 pKa = 4.1PTFTPTTKK13 pKa = 10.13RR14 pKa = 11.84GRR16 pKa = 11.84ALLLHH21 pKa = 5.75VLEE24 pKa = 5.66IIEE27 pKa = 4.31AEE29 pKa = 4.01ARR31 pKa = 11.84ADD33 pKa = 3.83GYY35 pKa = 11.84GDD37 pKa = 3.54IEE39 pKa = 5.06DD40 pKa = 3.97ISRR43 pKa = 11.84AGHH46 pKa = 6.15WNQGTWGEE54 pKa = 4.19VRR56 pKa = 11.84AEE58 pKa = 3.87DD59 pKa = 5.84LIAQGLDD66 pKa = 3.16FDD68 pKa = 5.14ALLTEE73 pKa = 5.05SGAEE77 pKa = 3.9PGRR80 pKa = 11.84AVDD83 pKa = 3.93LALPVSPVNLCGTACCFAGHH103 pKa = 5.57TTFQVGDD110 pKa = 3.85TPVVNVVANRR120 pKa = 11.84DD121 pKa = 3.71WINTTEE127 pKa = 4.07VLAIMDD133 pKa = 4.33VRR135 pKa = 11.84PVDD138 pKa = 3.62HH139 pKa = 7.66PGLMVPVSTRR149 pKa = 11.84AMEE152 pKa = 4.2LLGLEE157 pKa = 4.13SDD159 pKa = 3.88EE160 pKa = 5.49ADD162 pKa = 4.42LLFEE166 pKa = 5.91ADD168 pKa = 3.45NTLADD173 pKa = 3.54LRR175 pKa = 11.84VIIEE179 pKa = 4.08YY180 pKa = 8.6LTNGVGIVEE189 pKa = 4.56CDD191 pKa = 3.17ACGEE195 pKa = 4.31RR196 pKa = 11.84PWNCPYY202 pKa = 10.7RR203 pKa = 11.84GDD205 pKa = 4.17EE206 pKa = 4.2VCSVCDD212 pKa = 3.78EE213 pKa = 4.74HH214 pKa = 7.89PDD216 pKa = 3.33TCVCYY221 pKa = 10.82SEE223 pKa = 6.13DD224 pKa = 3.61EE225 pKa = 4.2

Molecular weight: 24.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WLB7|A0A5Q2WLB7_9CAUD Uncharacterized protein OS=Gordonia phage NHagos OX=2653746 GN=54 PE=4 SV=1
MM1 pKa = 8.05VSMAEE6 pKa = 4.0FSYY9 pKa = 10.31PPIMVRR15 pKa = 11.84SPAHH19 pKa = 5.98VADD22 pKa = 3.58QLKK25 pKa = 10.38RR26 pKa = 11.84RR27 pKa = 11.84LKK29 pKa = 9.74VACAEE34 pKa = 4.29DD35 pKa = 5.49GIAYY39 pKa = 7.52WQFIEE44 pKa = 4.43RR45 pKa = 11.84ALDD48 pKa = 3.47EE49 pKa = 5.05RR50 pKa = 11.84DD51 pKa = 3.03ATMAKK56 pKa = 8.81MRR58 pKa = 11.84HH59 pKa = 5.4PLDD62 pKa = 3.8RR63 pKa = 11.84AALSPKK69 pKa = 10.15AKK71 pKa = 9.67GRR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 3.42

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

18948

41

1956

231.1

24.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.233 ± 0.309

0.95 ± 0.144

7.236 ± 0.265

6.18 ± 0.339

2.47 ± 0.148

8.798 ± 0.361

1.815 ± 0.16

4.565 ± 0.293

2.565 ± 0.232

8.138 ± 0.199

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.243 ± 0.105

2.707 ± 0.216

5.404 ± 0.215

3.103 ± 0.171

6.803 ± 0.387

5.795 ± 0.248

6.84 ± 0.239

7.742 ± 0.179

2.021 ± 0.112

2.391 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski