Pigeon circovirus (PiCV) (Columbid circovirus)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Circovirus; Pigeon circovirus

Average proteome isoelectric point is 8.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9IG45|REP_PICV Replication-associated protein OS=Pigeon circovirus OX=126070 GN=rep PE=3 SV=1
MM1 pKa = 7.71RR2 pKa = 11.84VPVGHH7 pKa = 5.75TQQLTEE13 pKa = 4.1GQPAVEE19 pKa = 5.11VINDD23 pKa = 3.6DD24 pKa = 3.69YY25 pKa = 11.47FLVVVPVPPFTLHH38 pKa = 6.49LVEE41 pKa = 4.57FTSGVVSCPTALPATGRR58 pKa = 11.84ARR60 pKa = 11.84DD61 pKa = 3.72DD62 pKa = 3.6DD63 pKa = 4.09DD64 pKa = 4.94FRR66 pKa = 11.84FEE68 pKa = 4.26VTGLLTNQQLQVAQATPIFDD88 pKa = 3.61VYY90 pKa = 9.88LTEE93 pKa = 3.96VSRR96 pKa = 11.84DD97 pKa = 3.47LGHH100 pKa = 6.94CSSGFHH106 pKa = 6.87RR107 pKa = 11.84GNSLAEE113 pKa = 3.84IAPVSLRR120 pKa = 11.84RR121 pKa = 11.84NAQGKK126 pKa = 8.97

Molecular weight: 13.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9IG43|ORFC4_PICV Uncharacterized protein ORFC4 OS=Pigeon circovirus OX=126070 GN=ORFC4 PE=4 SV=1
MM1 pKa = 7.41RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84FRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84APIRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84IRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84TRR24 pKa = 11.84LSRR27 pKa = 11.84NIRR30 pKa = 11.84GHH32 pKa = 5.74RR33 pKa = 11.84RR34 pKa = 11.84SSRR37 pKa = 11.84IYY39 pKa = 9.05YY40 pKa = 9.71FRR42 pKa = 11.84LRR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 10.34DD47 pKa = 4.22KK48 pKa = 10.09ITLTQATNDD57 pKa = 3.58FKK59 pKa = 11.19FGTGIFTFKK68 pKa = 10.66LADD71 pKa = 3.57VLTVGLNAPTLKK83 pKa = 10.78VPFEE87 pKa = 5.07DD88 pKa = 3.91YY89 pKa = 10.86QIALVKK95 pKa = 10.82VEE97 pKa = 4.09MRR99 pKa = 11.84PLGVDD104 pKa = 2.6ITTWKK109 pKa = 10.66GFGHH113 pKa = 5.88TVPMYY118 pKa = 10.29DD119 pKa = 3.58ARR121 pKa = 11.84LKK123 pKa = 9.52TFQGQVDD130 pKa = 4.39LGDD133 pKa = 4.67DD134 pKa = 3.71PLMDD138 pKa = 4.22FDD140 pKa = 6.26GARR143 pKa = 11.84KK144 pKa = 8.46WDD146 pKa = 3.5LRR148 pKa = 11.84KK149 pKa = 10.26GFKK152 pKa = 10.14RR153 pKa = 11.84LIRR156 pKa = 11.84PRR158 pKa = 11.84PQLTIADD165 pKa = 4.52LATANQSAATWFSGRR180 pKa = 11.84NQWIPLQVSGNSLFPQKK197 pKa = 10.63VNHH200 pKa = 6.35YY201 pKa = 9.13GLAFSYY207 pKa = 9.02LQPQPDD213 pKa = 3.39PMYY216 pKa = 10.0YY217 pKa = 8.59EE218 pKa = 4.78CEE220 pKa = 3.75VTFYY224 pKa = 11.71VKK226 pKa = 10.49FRR228 pKa = 11.84QFAWTTLNVPPTPNIEE244 pKa = 4.11GMEE247 pKa = 4.2LMHH250 pKa = 7.05ICNGDD255 pKa = 3.82CNQCFADD262 pKa = 5.3ALDD265 pKa = 4.73PDD267 pKa = 4.17SAVDD271 pKa = 3.97SEE273 pKa = 4.46

Molecular weight: 31.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

895

82

317

179.0

20.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.598 ± 1.026

2.011 ± 0.325

5.14 ± 1.02

4.358 ± 1.328

5.363 ± 0.551

7.821 ± 1.031

2.458 ± 0.486

3.911 ± 0.453

5.14 ± 1.642

8.38 ± 0.825

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.676 ± 0.335

3.24 ± 0.328

6.145 ± 0.611

4.469 ± 0.876

8.492 ± 1.448

6.145 ± 2.26

5.922 ± 1.165

6.816 ± 1.098

2.011 ± 0.561

2.905 ± 0.536
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski