Corynebacterium kroppenstedtii (strain DSM 44385 / JCM 11950 / CIP 105744 / CCUG 35717)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Corynebacteriaceae; Corynebacterium; Corynebacterium kroppenstedtii

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2018 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4LH36|C4LH36_CORK4 Two-component system response regulator OS=Corynebacterium kroppenstedtii (strain DSM 44385 / JCM 11950 / CIP 105744 / CCUG 35717) OX=645127 GN=tcsR1 PE=4 SV=1
MM1 pKa = 7.36TNSIPDD7 pKa = 3.95DD8 pKa = 3.78NSAGDD13 pKa = 4.36DD14 pKa = 3.78SAATPSDD21 pKa = 3.98AEE23 pKa = 4.14PSTAHH28 pKa = 6.35VFMEE32 pKa = 4.35DD33 pKa = 3.33TEE35 pKa = 4.97EE36 pKa = 5.52DD37 pKa = 3.82IEE39 pKa = 4.34WPAGKK44 pKa = 10.06RR45 pKa = 11.84LLYY48 pKa = 11.21AMLDD52 pKa = 3.51AGLDD56 pKa = 3.48APYY59 pKa = 10.7GCTEE63 pKa = 4.47GEE65 pKa = 4.63CGACQCVVEE74 pKa = 4.38PHH76 pKa = 6.35NNATTHH82 pKa = 5.12MVHH85 pKa = 6.73NNVLDD90 pKa = 4.45EE91 pKa = 4.19YY92 pKa = 11.24DD93 pKa = 3.51IADD96 pKa = 4.81DD97 pKa = 3.87MTLACQTLSDD107 pKa = 3.95TPGGKK112 pKa = 9.37FDD114 pKa = 3.74VSYY117 pKa = 11.51DD118 pKa = 3.54FF119 pKa = 5.88

Molecular weight: 12.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4LGL3|C4LGL3_CORK4 Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT OS=Corynebacterium kroppenstedtii (strain DSM 44385 / JCM 11950 / CIP 105744 / CCUG 35717) OX=645127 GN=murT PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84SRR17 pKa = 11.84VHH19 pKa = 6.19GFRR22 pKa = 11.84SRR24 pKa = 11.84MSTRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.22GRR42 pKa = 11.84KK43 pKa = 9.07SLTAA47 pKa = 4.07

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2018

0

2018

705368

24

2538

349.5

37.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.737 ± 0.052

0.79 ± 0.014

6.796 ± 0.053

5.612 ± 0.055

3.079 ± 0.032

8.361 ± 0.05

2.469 ± 0.027

5.199 ± 0.042

3.49 ± 0.056

8.566 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.241 ± 0.024

3.089 ± 0.032

5.129 ± 0.034

3.165 ± 0.03

6.293 ± 0.054

7.091 ± 0.049

6.351 ± 0.037

7.956 ± 0.05

1.393 ± 0.024

2.192 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski