gamma proteobacterium BDW918

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Spongiibacteraceae; unclassified Spongiibacteraceae

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3794 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2JF95|I2JF95_9GAMM Uncharacterized protein OS=gamma proteobacterium BDW918 OX=1168065 GN=DOK_17700 PE=4 SV=1
MM1 pKa = 7.35FMSKK5 pKa = 7.11QWKK8 pKa = 8.35SALAAGVILASATGAAMAEE27 pKa = 4.07DD28 pKa = 4.45QEE30 pKa = 4.7TGFYY34 pKa = 10.67VGGNAFYY41 pKa = 11.4NEE43 pKa = 4.05VFDD46 pKa = 5.58ADD48 pKa = 3.78GTATTTTAGGFAAIPLIGDD67 pKa = 3.68VLNGLLGGSASSSANLEE84 pKa = 3.83TSYY87 pKa = 11.71DD88 pKa = 3.41NDD90 pKa = 2.76ISYY93 pKa = 10.75GFSVGYY99 pKa = 10.28KK100 pKa = 8.41FASPVRR106 pKa = 11.84IEE108 pKa = 3.61FEE110 pKa = 4.07YY111 pKa = 10.88RR112 pKa = 11.84QGEE115 pKa = 4.1NDD117 pKa = 2.9VDD119 pKa = 4.49KK120 pKa = 10.76ISSGGVSTTSDD131 pKa = 2.79SSLEE135 pKa = 4.16VTSMMGNVWYY145 pKa = 10.07DD146 pKa = 3.87FAAGEE151 pKa = 4.15RR152 pKa = 11.84LRR154 pKa = 11.84PYY156 pKa = 10.26IGFGLGMANLDD167 pKa = 4.06MGDD170 pKa = 3.87ADD172 pKa = 4.91DD173 pKa = 5.97DD174 pKa = 4.19VTIGQLGAGITYY186 pKa = 10.09FLTPRR191 pKa = 11.84LALDD195 pKa = 3.55AGYY198 pKa = 9.74RR199 pKa = 11.84YY200 pKa = 10.16SVAEE204 pKa = 4.05DD205 pKa = 3.42ATFKK209 pKa = 10.93SATIEE214 pKa = 3.97QEE216 pKa = 3.96NEE218 pKa = 3.46YY219 pKa = 10.38SAQSVQVGLRR229 pKa = 11.84YY230 pKa = 10.39NFFEE234 pKa = 4.1AQYY237 pKa = 10.75GVKK240 pKa = 10.47DD241 pKa = 3.55SDD243 pKa = 3.82GDD245 pKa = 4.18GVSDD249 pKa = 4.51EE250 pKa = 4.4TDD252 pKa = 3.23EE253 pKa = 5.44CPGTPRR259 pKa = 11.84GVQVDD264 pKa = 4.07SVGCPLDD271 pKa = 3.81GDD273 pKa = 3.96NDD275 pKa = 3.97GVADD279 pKa = 5.15YY280 pKa = 11.39LDD282 pKa = 3.62QCPNTPAGAKK292 pKa = 9.73VNASGCPLDD301 pKa = 4.24GDD303 pKa = 3.96NDD305 pKa = 4.07GVADD309 pKa = 5.33ADD311 pKa = 4.43DD312 pKa = 4.68ACPDD316 pKa = 3.72TPAGQAVMSNGCAKK330 pKa = 10.3DD331 pKa = 3.15QAVILRR337 pKa = 11.84GVNFEE342 pKa = 4.81LNSAQLTMNAEE353 pKa = 4.31TILNGVAEE361 pKa = 4.68TLVSSPGFDD370 pKa = 3.81VEE372 pKa = 4.64LQGHH376 pKa = 5.35TDD378 pKa = 3.22STGSDD383 pKa = 3.75SYY385 pKa = 12.21NMNLSQNRR393 pKa = 11.84AKK395 pKa = 10.43SVKK398 pKa = 10.02SYY400 pKa = 10.5LVSSGVEE407 pKa = 4.05ANRR410 pKa = 11.84LTARR414 pKa = 11.84GYY416 pKa = 11.14GEE418 pKa = 4.18EE419 pKa = 4.23QPIASNDD426 pKa = 3.75TKK428 pKa = 10.96DD429 pKa = 3.43GRR431 pKa = 11.84AQNRR435 pKa = 11.84RR436 pKa = 11.84VEE438 pKa = 4.27LKK440 pKa = 10.88VIGDD444 pKa = 4.09GSSDD448 pKa = 3.43SVEE451 pKa = 3.37PMMYY455 pKa = 10.04DD456 pKa = 2.98EE457 pKa = 5.38PMVEE461 pKa = 4.24EE462 pKa = 4.38PAVEE466 pKa = 4.33EE467 pKa = 4.14PMMDD471 pKa = 3.05EE472 pKa = 4.2PMVEE476 pKa = 4.22EE477 pKa = 4.26EE478 pKa = 4.88PMVEE482 pKa = 4.31DD483 pKa = 3.84EE484 pKa = 5.0PMMEE488 pKa = 4.13EE489 pKa = 4.28APAEE493 pKa = 4.2AEE495 pKa = 3.79PSVEE499 pKa = 5.52DD500 pKa = 4.11EE501 pKa = 4.39YY502 pKa = 11.28EE503 pKa = 4.02YY504 pKa = 11.37DD505 pKa = 3.64PSAEE509 pKa = 3.57TDD511 pKa = 3.68YY512 pKa = 11.98

Molecular weight: 54.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I2JER4|I2JER4_9GAMM Putative cytochrome oxidase assembly protein OS=gamma proteobacterium BDW918 OX=1168065 GN=DOK_18340 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.0KK13 pKa = 10.58ARR15 pKa = 11.84NHH17 pKa = 5.3GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.45NGRR29 pKa = 11.84KK30 pKa = 9.38LINRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.31GRR40 pKa = 11.84AVLSAA45 pKa = 3.96

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3794

0

3794

1259801

20

2945

332.1

36.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.344 ± 0.046

1.106 ± 0.014

5.813 ± 0.034

5.874 ± 0.031

3.838 ± 0.023

7.522 ± 0.038

2.158 ± 0.019

5.846 ± 0.027

4.215 ± 0.036

10.537 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.432 ± 0.023

3.8 ± 0.029

4.292 ± 0.026

4.095 ± 0.027

5.585 ± 0.038

6.678 ± 0.037

4.898 ± 0.043

6.809 ± 0.034

1.26 ± 0.016

2.899 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski