Verrucomicrobiales bacterium VVV1

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales; unclassified Verrucomicrobiales

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5352 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257LYI8|A0A257LYI8_9BACT Glucose-6-phosphate isomerase OS=Verrucomicrobiales bacterium VVV1 OX=2015575 GN=pgi PE=3 SV=1
EEE2 pKa = 4.13FATTDDD8 pKa = 2.74SGNYYY13 pKa = 9.35ISGLIAGTYYY23 pKa = 8.05VVVDDD28 pKa = 4.29TSLPAGVTVTGDDD41 pKa = 3.41DDD43 pKa = 3.5TKKK46 pKa = 10.64DD47 pKa = 3.19KKK49 pKa = 7.93TVTLTAAQALTTANFGYYY67 pKa = 10.17GNASFGDDD75 pKa = 4.25YY76 pKa = 9.82WNDDD80 pKa = 3.31NGDDD84 pKa = 4.22VQDDD88 pKa = 3.75TDDD91 pKa = 4.05PLSGVRR97 pKa = 11.84VFIDDD102 pKa = 3.62NANGTRR108 pKa = 11.84EEE110 pKa = 4.16NEEE113 pKa = 3.81FATTNASGAYYY124 pKa = 10.43MTGLAAGSYYY134 pKa = 8.52VAVDDD139 pKa = 3.57TSLPSGASQTGDDD152 pKa = 3.24DDD154 pKa = 3.58TKKK157 pKa = 10.79DD158 pKa = 3.03RR159 pKa = 11.84TAVTLTSGQALTTADDD175 pKa = 4.12GYYY178 pKa = 10.46GNASVGDDD186 pKa = 4.16YY187 pKa = 10.31WNDDD191 pKa = 3.03NGDDD195 pKa = 3.83GQGATEEE202 pKa = 4.25PMSGIRR208 pKa = 11.84VFIDDD213 pKa = 3.44NANGSRR219 pKa = 11.84DDD221 pKa = 3.67NEEE224 pKa = 4.23FATTNASGAYYY235 pKa = 7.99ISGLTPGTYYY245 pKa = 8.81VAVDDD250 pKa = 3.58TTLPSGTTNTGDDD263 pKa = 3.71DDD265 pKa = 3.61TKKK268 pKa = 10.63DD269 pKa = 3.15KKK271 pKa = 9.64SVTLAAGVPNTTSDDD286 pKa = 3.98GYYY289 pKa = 10.04GNGAISAIVFLDDD302 pKa = 3.58DDD304 pKa = 3.39SGAQTSGEEE313 pKa = 4.03GISGVTVFLDDD324 pKa = 3.65YY325 pKa = 11.42GDDD328 pKa = 4.16IRR330 pKa = 11.84QTNEEE335 pKa = 3.53QTTTSSTGAYYY346 pKa = 9.45FTGLIPGNYYY356 pKa = 8.62VVVVSATLPAGVTQTADDD374 pKa = 3.31DDD376 pKa = 3.72TMDDD380 pKa = 3.17KKK382 pKa = 8.58TVAVTAGATNTTPNFGYYY400 pKa = 10.22GNASIGDDD408 pKa = 4.17YY409 pKa = 10.91WIDDD413 pKa = 3.06DDD415 pKa = 4.36NGTQDDD421 pKa = 3.23TEEE424 pKa = 4.44PLGGVVVYYY433 pKa = 10.95DDD435 pKa = 4.21NSNGTRR441 pKa = 11.84EEE443 pKa = 4.29NEEE446 pKa = 3.5FATTNSSGSYYY457 pKa = 8.91INGLTPGTYYY467 pKa = 10.24VTVDDD472 pKa = 5.25STLPAGSTNTGDDD485 pKa = 3.84DDD487 pKa = 3.33TKKK490 pKa = 10.58DD491 pKa = 3.4KKK493 pKa = 9.55SVTLASAQSLTTADDD508 pKa = 3.62GYYY511 pKa = 10.53GVGSIGSSIWN

Molecular weight: 52.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257LEN3|A0A257LEN3_9BACT MFS transporter (Fragment) OS=Verrucomicrobiales bacterium VVV1 OX=2015575 GN=CFE26_28175 PE=4 SV=1
MM1 pKa = 7.66PPSSSRR7 pKa = 11.84PSKK10 pKa = 8.99PAKK13 pKa = 9.46RR14 pKa = 11.84ANSSRR19 pKa = 11.84KK20 pKa = 7.19TPSASSSATNGPSPPRR36 pKa = 11.84SSSSSSASGANSPSAPNPASSALHH60 pKa = 6.01ALRR63 pKa = 11.84SRR65 pKa = 11.84RR66 pKa = 11.84LPRR69 pKa = 11.84PPCPRR74 pKa = 11.84PPQPNRR80 pKa = 11.84PPPPPP85 pKa = 4.13

Molecular weight: 8.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5352

0

5352

1447052

20

2552

270.4

29.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.543 ± 0.038

0.927 ± 0.012

5.348 ± 0.025

5.713 ± 0.047

4.004 ± 0.023

8.618 ± 0.05

2.051 ± 0.02

4.906 ± 0.025

4.352 ± 0.041

10.046 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.071 ± 0.019

3.351 ± 0.035

5.396 ± 0.028

3.122 ± 0.019

6.072 ± 0.04

6.661 ± 0.039

6.245 ± 0.062

6.741 ± 0.031

1.589 ± 0.015

2.24 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski