Amino acid dipepetide frequency for Nitrosomonas stercoris

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.048AlaAla: 10.048 ± 0.171
0.984AlaCys: 0.984 ± 0.038
4.885AlaAsp: 4.885 ± 0.085
5.963AlaGlu: 5.963 ± 0.094
3.181AlaPhe: 3.181 ± 0.073
6.852AlaGly: 6.852 ± 0.116
2.301AlaHis: 2.301 ± 0.069
6.479AlaIle: 6.479 ± 0.112
3.876AlaLys: 3.876 ± 0.085
10.62AlaLeu: 10.62 ± 0.133
2.339AlaMet: 2.339 ± 0.059
3.329AlaAsn: 3.329 ± 0.081
3.051AlaPro: 3.051 ± 0.072
4.502AlaGln: 4.502 ± 0.088
5.368AlaArg: 5.368 ± 0.101
5.038AlaSer: 5.038 ± 0.086
5.108AlaThr: 5.108 ± 0.088
6.355AlaVal: 6.355 ± 0.115
1.226AlaTrp: 1.226 ± 0.045
2.392AlaTyr: 2.392 ± 0.061
0.0AlaXaa: 0.0 ± 0.0
Cys
0.83CysAla: 0.83 ± 0.031
0.104CysCys: 0.104 ± 0.014
0.521CysAsp: 0.521 ± 0.03
0.551CysGlu: 0.551 ± 0.024
0.408CysPhe: 0.408 ± 0.026
0.824CysGly: 0.824 ± 0.037
0.339CysHis: 0.339 ± 0.027
0.543CysIle: 0.543 ± 0.03
0.293CysLys: 0.293 ± 0.02
0.908CysLeu: 0.908 ± 0.042
0.189CysMet: 0.189 ± 0.018
0.312CysAsn: 0.312 ± 0.022
0.472CysPro: 0.472 ± 0.034
0.472CysGln: 0.472 ± 0.029
0.616CysArg: 0.616 ± 0.03
0.572CysSer: 0.572 ± 0.03
0.462CysThr: 0.462 ± 0.029
0.641CysVal: 0.641 ± 0.03
0.129CysTrp: 0.129 ± 0.013
0.298CysTyr: 0.298 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
4.483AspAla: 4.483 ± 0.078
0.49AspCys: 0.49 ± 0.029
2.457AspAsp: 2.457 ± 0.07
3.389AspGlu: 3.389 ± 0.072
2.322AspPhe: 2.322 ± 0.054
3.158AspGly: 3.158 ± 0.071
1.235AspHis: 1.235 ± 0.047
3.65AspIle: 3.65 ± 0.077
2.511AspLys: 2.511 ± 0.068
5.243AspLeu: 5.243 ± 0.088
1.223AspMet: 1.223 ± 0.047
2.04AspAsn: 2.04 ± 0.06
2.433AspPro: 2.433 ± 0.067
2.326AspGln: 2.326 ± 0.06
2.643AspArg: 2.643 ± 0.058
3.596AspSer: 3.596 ± 0.723
2.866AspThr: 2.866 ± 0.077
3.237AspVal: 3.237 ± 0.073
0.887AspTrp: 0.887 ± 0.04
1.929AspTyr: 1.929 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
5.579GluAla: 5.579 ± 0.111
0.418GluCys: 0.418 ± 0.022
2.631GluAsp: 2.631 ± 0.067
3.75GluGlu: 3.75 ± 0.096
2.163GluPhe: 2.163 ± 0.059
3.216GluGly: 3.216 ± 0.075
1.462GluHis: 1.462 ± 0.047
4.783GluIle: 4.783 ± 0.095
3.574GluLys: 3.574 ± 0.082
6.347GluLeu: 6.347 ± 0.112
1.543GluMet: 1.543 ± 0.046
2.44GluAsn: 2.44 ± 0.065
1.99GluPro: 1.99 ± 0.06
4.014GluGln: 4.014 ± 0.083
3.838GluArg: 3.838 ± 0.084
2.964GluSer: 2.964 ± 0.07
3.351GluThr: 3.351 ± 0.066
3.794GluVal: 3.794 ± 0.078
0.744GluTrp: 0.744 ± 0.036
1.599GluTyr: 1.599 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
3.442PheAla: 3.442 ± 0.078
0.497PheCys: 0.497 ± 0.031
2.436PheAsp: 2.436 ± 0.064
2.19PheGlu: 2.19 ± 0.057
1.792PhePhe: 1.792 ± 0.058
2.769PheGly: 2.769 ± 0.069
0.859PheHis: 0.859 ± 0.039
2.506PheIle: 2.506 ± 0.072
1.446PheLys: 1.446 ± 0.05
3.617PheLeu: 3.617 ± 0.081
0.906PheMet: 0.906 ± 0.035
1.562PheAsn: 1.562 ± 0.054
1.676PhePro: 1.676 ± 0.045
1.316PheGln: 1.316 ± 0.041
1.921PheArg: 1.921 ± 0.058
3.045PheSer: 3.045 ± 0.068
2.228PheThr: 2.228 ± 0.052
2.603PheVal: 2.603 ± 0.068
0.505PheTrp: 0.505 ± 0.03
1.31PheTyr: 1.31 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
5.47GlyAla: 5.47 ± 0.108
0.801GlyCys: 0.801 ± 0.032
3.152GlyAsp: 3.152 ± 0.077
4.026GlyGlu: 4.026 ± 0.075
2.993GlyPhe: 2.993 ± 0.077
4.781GlyGly: 4.781 ± 0.097
1.606GlyHis: 1.606 ± 0.058
5.061GlyIle: 5.061 ± 0.086
3.98GlyLys: 3.98 ± 0.084
6.824GlyLeu: 6.824 ± 0.091
2.0GlyMet: 2.0 ± 0.058
2.479GlyAsn: 2.479 ± 0.071
1.685GlyPro: 1.685 ± 0.05
2.707GlyGln: 2.707 ± 0.06
3.589GlyArg: 3.589 ± 0.077
3.917GlySer: 3.917 ± 0.076
3.508GlyThr: 3.508 ± 0.076
4.897GlyVal: 4.897 ± 0.097
1.025GlyTrp: 1.025 ± 0.043
2.399GlyTyr: 2.399 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
2.496HisAla: 2.496 ± 0.062
0.323HisCys: 0.323 ± 0.024
1.222HisAsp: 1.222 ± 0.042
1.291HisGlu: 1.291 ± 0.049
1.053HisPhe: 1.053 ± 0.037
1.797HisGly: 1.797 ± 0.054
0.925HisHis: 0.925 ± 0.042
1.619HisIle: 1.619 ± 0.053
0.991HisLys: 0.991 ± 0.044
2.581HisLeu: 2.581 ± 0.066
0.497HisMet: 0.497 ± 0.029
0.943HisAsn: 0.943 ± 0.038
1.521HisPro: 1.521 ± 0.056
1.163HisGln: 1.163 ± 0.042
1.279HisArg: 1.279 ± 0.047
1.459HisSer: 1.459 ± 0.045
1.571HisThr: 1.571 ± 0.049
1.439HisVal: 1.439 ± 0.049
0.34HisTrp: 0.34 ± 0.022
0.965HisTyr: 0.965 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
6.872IleAla: 6.872 ± 0.105
0.667IleCys: 0.667 ± 0.033
3.995IleAsp: 3.995 ± 0.083
4.444IleGlu: 4.444 ± 0.084
2.471IlePhe: 2.471 ± 0.064
4.758IleGly: 4.758 ± 0.093
1.641IleHis: 1.641 ± 0.05
4.184IleIle: 4.184 ± 0.088
3.174IleLys: 3.174 ± 0.086
6.259IleLeu: 6.259 ± 0.106
1.409IleMet: 1.409 ± 0.047
2.814IleAsn: 2.814 ± 0.058
3.232IlePro: 3.232 ± 0.071
2.762IleGln: 2.762 ± 0.081
3.763IleArg: 3.763 ± 0.077
4.29IleSer: 4.29 ± 0.083
4.038IleThr: 4.038 ± 0.08
4.425IleVal: 4.425 ± 0.073
0.732IleTrp: 0.732 ± 0.036
1.811IleTyr: 1.811 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
3.58LysAla: 3.58 ± 0.081
0.251LysCys: 0.251 ± 0.021
1.967LysAsp: 1.967 ± 0.058
2.647LysGlu: 2.647 ± 0.071
1.386LysPhe: 1.386 ± 0.048
2.483LysGly: 2.483 ± 0.071
1.148LysHis: 1.148 ± 0.044
3.518LysIle: 3.518 ± 0.086
2.597LysLys: 2.597 ± 0.071
5.186LysLeu: 5.186 ± 0.08
1.181LysMet: 1.181 ± 0.043
2.184LysAsn: 2.184 ± 0.059
1.961LysPro: 1.961 ± 0.052
2.745LysGln: 2.745 ± 0.071
2.92LysArg: 2.92 ± 0.073
2.536LysSer: 2.536 ± 0.064
2.74LysThr: 2.74 ± 0.07
2.659LysVal: 2.659 ± 0.065
0.613LysTrp: 0.613 ± 0.031
1.137LysTyr: 1.137 ± 0.039
0.0LysXaa: 0.0 ± 0.0
Leu
11.225LeuAla: 11.225 ± 0.15
1.025LeuCys: 1.025 ± 0.038
5.748LeuAsp: 5.748 ± 0.092
6.29LeuGlu: 6.29 ± 0.106
4.071LeuPhe: 4.071 ± 0.092
6.884LeuGly: 6.884 ± 0.108
2.555LeuHis: 2.555 ± 0.067
6.742LeuIle: 6.742 ± 0.113
4.629LeuLys: 4.629 ± 0.085
12.272LeuLeu: 12.272 ± 0.191
2.297LeuMet: 2.297 ± 0.057
3.829LeuAsn: 3.829 ± 0.073
5.614LeuPro: 5.614 ± 0.085
4.986LeuGln: 4.986 ± 0.118
6.245LeuArg: 6.245 ± 0.109
6.874LeuSer: 6.874 ± 0.124
6.001LeuThr: 6.001 ± 0.091
6.484LeuVal: 6.484 ± 0.104
1.211LeuTrp: 1.211 ± 0.047
2.722LeuTyr: 2.722 ± 0.074
0.0LeuXaa: 0.0 ± 0.0
Met
2.291MetAla: 2.291 ± 0.065
0.142MetCys: 0.142 ± 0.016
1.141MetAsp: 1.141 ± 0.04
1.377MetGlu: 1.377 ± 0.046
0.749MetPhe: 0.749 ± 0.033
1.431MetGly: 1.431 ± 0.053
0.59MetHis: 0.59 ± 0.03
1.472MetIle: 1.472 ± 0.055
1.201MetLys: 1.201 ± 0.042
2.91MetLeu: 2.91 ± 0.065
0.566MetMet: 0.566 ± 0.028
0.993MetAsn: 0.993 ± 0.034
1.142MetPro: 1.142 ± 0.045
1.274MetGln: 1.274 ± 0.045
1.541MetArg: 1.541 ± 0.049
1.327MetSer: 1.327 ± 0.044
1.521MetThr: 1.521 ± 0.051
1.519MetVal: 1.519 ± 0.044
0.176MetTrp: 0.176 ± 0.018
0.421MetTyr: 0.421 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
2.968AsnAla: 2.968 ± 0.072
0.383AsnCys: 0.383 ± 0.026
1.684AsnAsp: 1.684 ± 0.048
2.043AsnGlu: 2.043 ± 0.057
1.481AsnPhe: 1.481 ± 0.046
2.486AsnGly: 2.486 ± 0.061
0.974AsnHis: 0.974 ± 0.037
2.663AsnIle: 2.663 ± 0.063
1.89AsnLys: 1.89 ± 0.053
4.023AsnLeu: 4.023 ± 0.074
0.937AsnMet: 0.937 ± 0.038
1.687AsnAsn: 1.687 ± 0.057
2.1AsnPro: 2.1 ± 0.057
2.019AsnGln: 2.019 ± 0.06
2.147AsnArg: 2.147 ± 0.062
2.141AsnSer: 2.141 ± 0.074
2.22AsnThr: 2.22 ± 0.061
2.26AsnVal: 2.26 ± 0.066
0.557AsnTrp: 0.557 ± 0.031
1.186AsnTyr: 1.186 ± 0.043
0.0AsnXaa: 0.0 ± 0.0
Pro
4.067ProAla: 4.067 ± 0.088
0.323ProCys: 0.323 ± 0.021
2.96ProAsp: 2.96 ± 0.077
3.376ProGlu: 3.376 ± 0.07
1.687ProPhe: 1.687 ± 0.046
3.127ProGly: 3.127 ± 0.072
1.075ProHis: 1.075 ± 0.041
2.571ProIle: 2.571 ± 0.064
1.603ProLys: 1.603 ± 0.048
4.324ProLeu: 4.324 ± 0.08
0.846ProMet: 0.846 ± 0.036
1.641ProAsn: 1.641 ± 0.053
1.695ProPro: 1.695 ± 0.054
1.882ProGln: 1.882 ± 0.055
1.838ProArg: 1.838 ± 0.053
2.291ProSer: 2.291 ± 0.061
2.292ProThr: 2.292 ± 0.074
3.435ProVal: 3.435 ± 0.082
0.543ProTrp: 0.543 ± 0.029
1.27ProTyr: 1.27 ± 0.045
0.0ProXaa: 0.0 ± 0.0
Gln
5.105GlnAla: 5.105 ± 0.111
0.402GlnCys: 0.402 ± 0.023
2.08GlnAsp: 2.08 ± 0.055
2.737GlnGlu: 2.737 ± 0.073
1.726GlnPhe: 1.726 ± 0.054
2.8GlnGly: 2.8 ± 0.066
1.572GlnHis: 1.572 ± 0.052
3.064GlnIle: 3.064 ± 0.066
1.974GlnLys: 1.974 ± 0.074
5.774GlnLeu: 5.774 ± 0.111
1.018GlnMet: 1.018 ± 0.04
1.537GlnAsn: 1.537 ± 0.053
2.307GlnPro: 2.307 ± 0.063
3.633GlnGln: 3.633 ± 0.103
2.894GlnArg: 2.894 ± 0.07
2.524GlnSer: 2.524 ± 0.059
2.581GlnThr: 2.581 ± 0.063
3.08GlnVal: 3.08 ± 0.071
0.653GlnTrp: 0.653 ± 0.028
1.26GlnTyr: 1.26 ± 0.052
0.0GlnXaa: 0.0 ± 0.0
Arg
4.873ArgAla: 4.873 ± 0.101
0.527ArgCys: 0.527 ± 0.031
2.784ArgAsp: 2.784 ± 0.06
3.677ArgGlu: 3.677 ± 0.082
2.462ArgPhe: 2.462 ± 0.064
3.171ArgGly: 3.171 ± 0.074
1.65ArgHis: 1.65 ± 0.055
4.013ArgIle: 4.013 ± 0.086
2.76ArgLys: 2.76 ± 0.068
6.1ArgLeu: 6.1 ± 0.109
1.562ArgMet: 1.562 ± 0.045
2.247ArgAsn: 2.247 ± 0.053
2.062ArgPro: 2.062 ± 0.055
2.64ArgGln: 2.64 ± 0.063
3.197ArgArg: 3.197 ± 0.08
3.251ArgSer: 3.251 ± 0.083
2.939ArgThr: 2.939 ± 0.071
3.568ArgVal: 3.568 ± 0.073
0.812ArgTrp: 0.812 ± 0.033
2.16ArgTyr: 2.16 ± 0.06
0.0ArgXaa: 0.0 ± 0.0
Ser
5.093SerAla: 5.093 ± 0.084
0.56SerCys: 0.56 ± 0.032
3.882SerAsp: 3.882 ± 0.728
3.24SerGlu: 3.24 ± 0.074
2.244SerPhe: 2.244 ± 0.058
5.019SerGly: 5.019 ± 0.086
1.502SerHis: 1.502 ± 0.047
3.903SerIle: 3.903 ± 0.083
2.511SerLys: 2.511 ± 0.067
5.95SerLeu: 5.95 ± 0.1
1.566SerMet: 1.566 ± 0.056
2.242SerAsn: 2.242 ± 0.064
2.392SerPro: 2.392 ± 0.064
2.462SerGln: 2.462 ± 0.063
3.187SerArg: 3.187 ± 0.068
3.841SerSer: 3.841 ± 0.084
3.246SerThr: 3.246 ± 0.078
3.873SerVal: 3.873 ± 0.085
0.771SerTrp: 0.771 ± 0.036
1.643SerTyr: 1.643 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
5.445ThrAla: 5.445 ± 0.093
0.493ThrCys: 0.493 ± 0.03
3.012ThrAsp: 3.012 ± 0.073
3.099ThrGlu: 3.099 ± 0.062
1.981ThrPhe: 1.981 ± 0.051
4.403ThrGly: 4.403 ± 0.085
1.502ThrHis: 1.502 ± 0.05
3.848ThrIle: 3.848 ± 0.081
2.012ThrLys: 2.012 ± 0.06
6.544ThrLeu: 6.544 ± 0.113
1.081ThrMet: 1.081 ± 0.043
1.861ThrAsn: 1.861 ± 0.054
2.882ThrPro: 2.882 ± 0.059
2.569ThrGln: 2.569 ± 0.066
3.048ThrArg: 3.048 ± 0.064
3.144ThrSer: 3.144 ± 0.065
3.372ThrThr: 3.372 ± 0.074
3.823ThrVal: 3.823 ± 0.088
0.626ThrTrp: 0.626 ± 0.035
1.446ThrTyr: 1.446 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
6.551ValAla: 6.551 ± 0.104
0.612ValCys: 0.612 ± 0.032
3.555ValAsp: 3.555 ± 0.074
4.002ValGlu: 4.002 ± 0.091
2.622ValPhe: 2.622 ± 0.068
4.158ValGly: 4.158 ± 0.096
1.368ValHis: 1.368 ± 0.048
4.753ValIle: 4.753 ± 0.093
2.88ValLys: 2.88 ± 0.068
6.975ValLeu: 6.975 ± 0.117
1.753ValMet: 1.753 ± 0.051
2.305ValAsn: 2.305 ± 0.062
2.713ValPro: 2.713 ± 0.059
2.499ValGln: 2.499 ± 0.06
3.386ValArg: 3.386 ± 0.078
3.875ValSer: 3.875 ± 0.066
4.124ValThr: 4.124 ± 0.09
4.799ValVal: 4.799 ± 0.092
0.711ValTrp: 0.711 ± 0.033
1.78ValTyr: 1.78 ± 0.05
0.0ValXaa: 0.0 ± 0.0
Trp
0.786TrpAla: 0.786 ± 0.036
0.157TrpCys: 0.157 ± 0.016
0.518TrpAsp: 0.518 ± 0.031
0.654TrpGlu: 0.654 ± 0.03
0.534TrpPhe: 0.534 ± 0.024
0.76TrpGly: 0.76 ± 0.032
0.352TrpHis: 0.352 ± 0.026
0.84TrpIle: 0.84 ± 0.033
0.532TrpLys: 0.532 ± 0.025
1.958TrpLeu: 1.958 ± 0.064
0.349TrpMet: 0.349 ± 0.023
0.462TrpAsn: 0.462 ± 0.027
0.525TrpPro: 0.525 ± 0.031
0.988TrpGln: 0.988 ± 0.041
0.965TrpArg: 0.965 ± 0.045
0.692TrpSer: 0.692 ± 0.031
0.469TrpThr: 0.469 ± 0.029
0.829TrpVal: 0.829 ± 0.041
0.226TrpTrp: 0.226 ± 0.02
0.343TrpTyr: 0.343 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.69TyrAla: 2.69 ± 0.065
0.327TyrCys: 0.327 ± 0.021
1.49TyrAsp: 1.49 ± 0.054
1.455TyrGlu: 1.455 ± 0.046
1.298TyrPhe: 1.298 ± 0.045
2.027TyrGly: 2.027 ± 0.058
0.818TyrHis: 0.818 ± 0.037
1.528TyrIle: 1.528 ± 0.051
1.003TyrLys: 1.003 ± 0.04
3.291TyrLeu: 3.291 ± 0.074
0.522TyrMet: 0.522 ± 0.028
0.943TyrAsn: 0.943 ± 0.042
1.389TyrPro: 1.389 ± 0.046
1.757TyrGln: 1.757 ± 0.051
2.071TyrArg: 2.071 ± 0.066
1.75TyrSer: 1.75 ± 0.046
1.556TyrThr: 1.556 ± 0.054
1.704TyrVal: 1.704 ± 0.051
0.452TyrTrp: 0.452 ± 0.029
1.006TyrTyr: 1.006 ± 0.042
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2150 proteins (681854 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski