Pusillimonas thiosulfatoxidans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Pusillimonas

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3284 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A410GAM8|A0A410GAM8_9BURK Poly-beta-1 6-N-acetyl-D-glucosamine biosynthesis protein PgaD OS=Pusillimonas thiosulfatoxidans OX=2028345 GN=pgaD PE=4 SV=1
MM1 pKa = 7.49SIKK4 pKa = 10.57LSALALGLTLAYY16 pKa = 10.08GATAALAADD25 pKa = 4.16VATVDD30 pKa = 4.56QNGNFNNAVIEE41 pKa = 4.09QWSNNTAHH49 pKa = 6.61ATITSYY55 pKa = 11.96GDD57 pKa = 3.28FNEE60 pKa = 5.8GYY62 pKa = 9.81IEE64 pKa = 4.38QNDD67 pKa = 3.61NTSAVATITTFGDD80 pKa = 3.35FNDD83 pKa = 3.99AVITQEE89 pKa = 5.23DD90 pKa = 4.53VRR92 pKa = 11.84TSNATIYY99 pKa = 10.04QAANAGSATIDD110 pKa = 3.3QSGGGWGGWWGSSDD124 pKa = 4.15GNNQNAVINQTAGWGNNAWVTQTGDD149 pKa = 3.42NLDD152 pKa = 4.63AIINQAGYY160 pKa = 10.02RR161 pKa = 11.84NQAGIVQDD169 pKa = 3.93GDD171 pKa = 3.39GGQYY175 pKa = 11.0NMARR179 pKa = 11.84INQSGSFNDD188 pKa = 5.43GYY190 pKa = 10.63ISQNGKK196 pKa = 9.23NLVATVSQYY205 pKa = 11.26GSHH208 pKa = 6.5NDD210 pKa = 3.39AVVLQQGANYY220 pKa = 7.43TATVTQVGYY229 pKa = 11.48GNDD232 pKa = 3.14AYY234 pKa = 11.57VNQRR238 pKa = 3.1

Molecular weight: 24.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A410GF55|A0A410GF55_9BURK Chromosome partitioning protein OS=Pusillimonas thiosulfatoxidans OX=2028345 GN=CKA81_14440 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.03GRR39 pKa = 11.84KK40 pKa = 8.03QLAVV44 pKa = 3.26

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3284

0

3284

1077498

32

3648

328.1

35.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.785 ± 0.059

0.914 ± 0.014

5.443 ± 0.038

5.216 ± 0.042

3.54 ± 0.027

8.106 ± 0.043

2.264 ± 0.023

5.182 ± 0.032

3.218 ± 0.036

10.741 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.652 ± 0.02

2.797 ± 0.021

5.015 ± 0.029

4.152 ± 0.029

6.489 ± 0.044

5.719 ± 0.028

5.267 ± 0.026

7.561 ± 0.037

1.425 ± 0.019

2.512 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski