Pseudomonas sp. BAY1663

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5053 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W9SY05|W9SY05_9PSED Uncharacterized protein OS=Pseudomonas sp. BAY1663 OX=1439940 GN=BAY1663_04462 PE=4 SV=1
MM1 pKa = 7.87ASTLDD6 pKa = 3.39ISNEE10 pKa = 3.49DD11 pKa = 3.51FAAGNYY17 pKa = 8.82RR18 pKa = 11.84FKK20 pKa = 11.35GDD22 pKa = 3.37GSNASVINQGTLMASDD38 pKa = 4.31GGAVALLGGTVSNQGVIVANSGTVALAAGNAVTLDD73 pKa = 3.62FAGDD77 pKa = 3.48GLLNVQVDD85 pKa = 4.3EE86 pKa = 4.31ATKK89 pKa = 10.72DD90 pKa = 3.37ALVEE94 pKa = 3.85NRR96 pKa = 11.84QLIQADD102 pKa = 4.06GGQVLLTAQAADD114 pKa = 3.25ALLRR118 pKa = 11.84TVVNNDD124 pKa = 3.7GIIEE128 pKa = 4.11ARR130 pKa = 11.84TLGEE134 pKa = 4.06KK135 pKa = 9.92NGKK138 pKa = 9.11VVLNGGDD145 pKa = 3.37EE146 pKa = 4.46GSVQVAGTLDD156 pKa = 3.47TSATTGQGGAIEE168 pKa = 4.16VTGANVALADD178 pKa = 3.78ATLDD182 pKa = 3.45ASGATGGGSVKK193 pKa = 10.73VGGDD197 pKa = 3.22WQGTGTLTRR206 pKa = 11.84AQATTVDD213 pKa = 3.41AASTLKK219 pKa = 10.73ADD221 pKa = 3.72ATHH224 pKa = 6.61NGDD227 pKa = 3.14GGTVVVWSDD236 pKa = 2.94QHH238 pKa = 5.8TSYY241 pKa = 10.49QGHH244 pKa = 6.82ISAKK248 pKa = 10.26GGAQGGDD255 pKa = 3.0GGQAEE260 pKa = 4.57VSGKK264 pKa = 10.45AVLGFTGTVDD274 pKa = 3.78LSAAHH279 pKa = 6.53GAFGDD284 pKa = 4.27LLLDD288 pKa = 4.72PYY290 pKa = 11.32DD291 pKa = 3.79LTISSGTGGSATATADD307 pKa = 4.09DD308 pKa = 4.21STLGADD314 pKa = 3.59TLEE317 pKa = 4.5GLLADD322 pKa = 4.29ANVTVSTGTAGSQNGDD338 pKa = 2.95ITVASAVSWDD348 pKa = 3.59ANTTLTLNAAGNIDD362 pKa = 3.51IDD364 pKa = 3.98AAITASGEE372 pKa = 4.41SAGLALNYY380 pKa = 10.59GGTEE384 pKa = 4.2GVAGATAASGTDD396 pKa = 3.55YY397 pKa = 11.25NVNAPVTLSGADD409 pKa = 3.1ATLSINGSDD418 pKa = 3.51YY419 pKa = 11.3TLIHH423 pKa = 7.68SMEE426 pKa = 4.1QLDD429 pKa = 5.13AIDD432 pKa = 3.67STGLSGHH439 pKa = 6.0YY440 pKa = 10.54ALAQDD445 pKa = 5.18LDD447 pKa = 4.45ASDD450 pKa = 4.28SPYY453 pKa = 10.72TSALVGIDD461 pKa = 3.25AANAFTGTFAGLGHH475 pKa = 7.71AIGNLTISASGSDD488 pKa = 3.82YY489 pKa = 11.49VGLFGYY495 pKa = 8.39TGSGSTLRR503 pKa = 11.84DD504 pKa = 3.03VGLQGGSVTGNSYY517 pKa = 10.94VGGLVGYY524 pKa = 9.93NDD526 pKa = 4.03GGSIVDD532 pKa = 4.37AYY534 pKa = 10.52ATVDD538 pKa = 3.18VTGAHH543 pKa = 4.55VTGGLVGANVGGGSILRR560 pKa = 11.84AYY562 pKa = 7.99ATGWVNATASEE573 pKa = 4.19GLGWAGGLIGQNAGSISQSYY593 pKa = 10.58ASGGVTGDD601 pKa = 3.71YY602 pKa = 10.88YY603 pKa = 11.47VGGLVGHH610 pKa = 6.77NGNGTVTDD618 pKa = 4.47SYY620 pKa = 12.07ALGNVHH626 pKa = 6.12GQWDD630 pKa = 3.67VGGG633 pKa = 3.82

Molecular weight: 61.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W9T8H7|W9T8H7_9PSED Two-component response regulator OS=Pseudomonas sp. BAY1663 OX=1439940 GN=BAY1663_03887 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29NGRR28 pKa = 11.84QVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5053

0

5053

1391436

29

2815

275.4

30.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.504 ± 0.051

1.09 ± 0.013

5.295 ± 0.025

6.091 ± 0.036

3.486 ± 0.021

8.104 ± 0.034

2.302 ± 0.019

4.603 ± 0.025

3.046 ± 0.031

11.939 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.305 ± 0.018

2.69 ± 0.022

5.008 ± 0.027

4.475 ± 0.033

7.327 ± 0.036

5.522 ± 0.027

4.519 ± 0.026

6.813 ± 0.032

1.452 ± 0.016

2.428 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski