Sphingomonas sp. KC8

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3846 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9YFJ7|A0A1X9YFJ7_9SPHN Uncharacterized protein OS=Sphingomonas sp. KC8 OX=1030157 GN=KC8_09580 PE=4 SV=1
MM1 pKa = 7.28FKK3 pKa = 10.74SSGGGNDD10 pKa = 3.35VLHH13 pKa = 6.88GGDD16 pKa = 3.44GTIVRR21 pKa = 11.84SYY23 pKa = 11.21LSDD26 pKa = 3.38GRR28 pKa = 11.84DD29 pKa = 2.98TVLYY33 pKa = 10.13YY34 pKa = 10.77GYY36 pKa = 10.7DD37 pKa = 3.23SVAIKK42 pKa = 10.64DD43 pKa = 4.51DD44 pKa = 3.99GNHH47 pKa = 5.66WSVEE51 pKa = 3.8KK52 pKa = 10.23TLDD55 pKa = 3.4YY56 pKa = 11.47EE57 pKa = 4.56EE58 pKa = 4.57YY59 pKa = 10.44TDD61 pKa = 3.49TLYY64 pKa = 11.01SIEE67 pKa = 4.32SFGSKK72 pKa = 9.29IFQIINKK79 pKa = 9.04IDD81 pKa = 3.67FSDD84 pKa = 3.74ASSGKK89 pKa = 9.37HH90 pKa = 5.68FKK92 pKa = 10.61DD93 pKa = 3.52ATDD96 pKa = 3.57SDD98 pKa = 3.94IDD100 pKa = 3.84DD101 pKa = 4.08AMEE104 pKa = 4.05TVGDD108 pKa = 4.3GIEE111 pKa = 4.1SFVRR115 pKa = 11.84VDD117 pKa = 3.01VDD119 pKa = 3.99GIEE122 pKa = 4.02VEE124 pKa = 4.38FFNFGTYY131 pKa = 9.72IGTTYY136 pKa = 11.4ADD138 pKa = 3.97RR139 pKa = 11.84FDD141 pKa = 4.54LNQIIGRR148 pKa = 11.84VFDD151 pKa = 4.14GGSGKK156 pKa = 10.19DD157 pKa = 3.25VIDD160 pKa = 3.74YY161 pKa = 11.33SEE163 pKa = 4.43VNDD166 pKa = 3.8TFVVNMRR173 pKa = 11.84PDD175 pKa = 3.05QRR177 pKa = 11.84IAYY180 pKa = 8.55ASSAPSVTDD189 pKa = 3.93SIQSIEE195 pKa = 4.29TVIGAQDD202 pKa = 3.34HH203 pKa = 6.71ANTFVGALGSLDD215 pKa = 4.18EE216 pKa = 4.83NDD218 pKa = 3.44PTFMGGAYY226 pKa = 9.41FANNWGYY233 pKa = 11.57SNLLTYY239 pKa = 10.75VGGSQADD246 pKa = 3.71TYY248 pKa = 11.42EE249 pKa = 4.74FDD251 pKa = 4.27FEE253 pKa = 5.12DD254 pKa = 4.23DD255 pKa = 3.49KK256 pKa = 11.82GVVYY260 pKa = 10.64IRR262 pKa = 11.84DD263 pKa = 3.79NGSGDD268 pKa = 3.56TDD270 pKa = 3.17RR271 pKa = 11.84VAISNVPPGWIDD283 pKa = 3.43EE284 pKa = 4.51STSMHH289 pKa = 6.62HH290 pKa = 6.66GFMVLQNIEE299 pKa = 4.13KK300 pKa = 10.66DD301 pKa = 3.12GDD303 pKa = 4.14NIVSLTIFRR312 pKa = 11.84EE313 pKa = 4.47DD314 pKa = 3.88YY315 pKa = 10.21LTGQSLQLRR324 pKa = 11.84ISSDD328 pKa = 3.03IEE330 pKa = 3.88EE331 pKa = 4.29IEE333 pKa = 4.45INGLTIGMANLLMSMHH349 pKa = 6.36YY350 pKa = 10.6NSGAYY355 pKa = 8.9YY356 pKa = 10.0VAPLDD361 pKa = 4.11AYY363 pKa = 10.99VGLSLARR370 pKa = 11.84LHH372 pKa = 6.18DD373 pKa = 3.74TVYY376 pKa = 11.27NSGPINNGEE385 pKa = 4.26SYY387 pKa = 11.17DD388 pKa = 3.94EE389 pKa = 4.18NLAPTFDD396 pKa = 4.82SNGQSTGVAVSSAIGGTWTTPEE418 pKa = 3.81IATEE422 pKa = 4.2SHH424 pKa = 6.64HH425 pKa = 5.9GWMVTGVTSDD435 pKa = 3.1SGYY438 pKa = 6.97TTLNTTSALKK448 pKa = 10.49EE449 pKa = 3.73IFFAEE454 pKa = 5.65GITAEE459 pKa = 4.39DD460 pKa = 3.51VRR462 pKa = 11.84FTATSSGVDD471 pKa = 3.29NAGLTIHH478 pKa = 7.4IDD480 pKa = 3.21RR481 pKa = 11.84LNYY484 pKa = 9.43SLSVAGFEE492 pKa = 4.64GGRR495 pKa = 11.84TVSGIGFYY503 pKa = 11.02DD504 pKa = 3.74SALHH508 pKa = 5.75AHH510 pKa = 6.32VSSAAAVSLVGTAPGQYY527 pKa = 8.25QVRR530 pKa = 11.84LSGEE534 pKa = 3.84TSYY537 pKa = 11.26TPNAVSATYY546 pKa = 10.09FLEE549 pKa = 4.2ALNFADD555 pKa = 3.88STVIDD560 pKa = 4.98LLNAPLTFKK569 pKa = 10.98GSALNEE575 pKa = 3.97TLYY578 pKa = 11.46GLNTRR583 pKa = 11.84GDD585 pKa = 4.55LIFGLAGNDD594 pKa = 3.52TIYY597 pKa = 10.67GYY599 pKa = 11.25GGADD603 pKa = 3.41VLHH606 pKa = 6.86GGDD609 pKa = 4.41GADD612 pKa = 3.91ALHH615 pKa = 6.81GGDD618 pKa = 5.51GNDD621 pKa = 3.35TLYY624 pKa = 11.45GDD626 pKa = 4.22GGIDD630 pKa = 3.27ALYY633 pKa = 10.42GGEE636 pKa = 4.45GNDD639 pKa = 3.35VLYY642 pKa = 11.19GGDD645 pKa = 4.32GNDD648 pKa = 3.81NLNGEE653 pKa = 4.45NGNDD657 pKa = 3.83TLHH660 pKa = 6.89GGAGNDD666 pKa = 3.47NLRR669 pKa = 11.84GGTGDD674 pKa = 3.44DD675 pKa = 3.46TYY677 pKa = 11.91VFARR681 pKa = 11.84VFSATTAGDD690 pKa = 3.88IIYY693 pKa = 10.34EE694 pKa = 4.07NAADD698 pKa = 4.06GFDD701 pKa = 3.93TIFFTDD707 pKa = 5.12GIEE710 pKa = 4.03ADD712 pKa = 4.46EE713 pKa = 4.63VYY715 pKa = 10.69SWTDD719 pKa = 2.83TSGFLWMQVGGAAATNTLKK738 pKa = 11.07AQGSYY743 pKa = 10.57AAATGITTRR752 pKa = 11.84IEE754 pKa = 3.98QVTFDD759 pKa = 5.32DD760 pKa = 3.79ATVWDD765 pKa = 4.63LTQGLHH771 pKa = 6.52LRR773 pKa = 11.84NNDD776 pKa = 3.29TARR779 pKa = 11.84ALYY782 pKa = 8.02GTIYY786 pKa = 10.89ADD788 pKa = 3.79TIEE791 pKa = 4.92GGSAADD797 pKa = 3.81NIYY800 pKa = 11.1GLGGNDD806 pKa = 3.34TLIGHH811 pKa = 7.42GGNDD815 pKa = 3.69ALRR818 pKa = 11.84GGAGDD823 pKa = 3.68DD824 pKa = 3.54TYY826 pKa = 11.52IFARR830 pKa = 11.84NFSGTTAGDD839 pKa = 3.86IIYY842 pKa = 10.59EE843 pKa = 4.18NVDD846 pKa = 3.5EE847 pKa = 5.03GNDD850 pKa = 3.78TIFFTDD856 pKa = 5.36GIDD859 pKa = 3.44ASEE862 pKa = 4.52VYY864 pKa = 10.25SWTDD868 pKa = 2.81TSGFLWMQVGSAAATNTLKK887 pKa = 11.07AQGSYY892 pKa = 10.87AAVTGITTRR901 pKa = 11.84IEE903 pKa = 3.88QVVFDD908 pKa = 5.87DD909 pKa = 3.73EE910 pKa = 4.98TVWDD914 pKa = 4.24LTQGLHH920 pKa = 6.52LRR922 pKa = 11.84NNDD925 pKa = 3.43TARR928 pKa = 11.84TFYY931 pKa = 9.89GTVYY935 pKa = 11.09GDD937 pKa = 3.59TLEE940 pKa = 4.85GGSASDD946 pKa = 4.27HH947 pKa = 6.57IYY949 pKa = 11.26GLGGNDD955 pKa = 3.34TLIGHH960 pKa = 7.36GGNDD964 pKa = 3.64TLRR967 pKa = 11.84GGDD970 pKa = 3.57GDD972 pKa = 3.91DD973 pKa = 3.5TYY975 pKa = 12.1VFATGFSLNATGDD988 pKa = 4.16RR989 pKa = 11.84IVEE992 pKa = 4.27TATGGTDD999 pKa = 3.43TILFTDD1005 pKa = 5.84GIGADD1010 pKa = 5.57DD1011 pKa = 3.95MFSWTDD1017 pKa = 3.07TSGNLWLQLAANPVSNTLKK1036 pKa = 10.28IDD1038 pKa = 3.65GAYY1041 pKa = 10.01SASTGITTYY1050 pKa = 10.82VEE1052 pKa = 3.95NVVFDD1057 pKa = 5.65DD1058 pKa = 3.53ATAWDD1063 pKa = 4.38LTQGLYY1069 pKa = 10.64LRR1071 pKa = 11.84NNDD1074 pKa = 3.94TGRR1077 pKa = 11.84QMYY1080 pKa = 10.36GSALGDD1086 pKa = 3.86WIEE1089 pKa = 4.57GGAGADD1095 pKa = 3.51TLRR1098 pKa = 11.84GFGGDD1103 pKa = 3.28DD1104 pKa = 3.34TLIGRR1109 pKa = 11.84GGNEE1113 pKa = 3.92DD1114 pKa = 4.11LYY1116 pKa = 11.09GGSGADD1122 pKa = 2.95TFVFRR1127 pKa = 11.84AVDD1130 pKa = 3.4VGNGIDD1136 pKa = 3.59TLRR1139 pKa = 11.84DD1140 pKa = 3.33FSMADD1145 pKa = 3.02NDD1147 pKa = 4.43VIDD1150 pKa = 5.11LRR1152 pKa = 11.84DD1153 pKa = 3.61VLDD1156 pKa = 4.28GGFDD1160 pKa = 3.66PMTDD1164 pKa = 3.18ALSDD1168 pKa = 3.7FVQFTNAGSHH1178 pKa = 4.75SQLRR1182 pKa = 11.84VDD1184 pKa = 4.76LDD1186 pKa = 4.03GAGTLYY1192 pKa = 10.73GWTHH1196 pKa = 6.6IATVNGHH1203 pKa = 5.78TNLDD1207 pKa = 3.64ATTLEE1212 pKa = 4.24ANGYY1216 pKa = 9.43LLAAA1220 pKa = 5.2

Molecular weight: 129.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9YA53|A0A1X9YA53_9SPHN Uncharacterized protein OS=Sphingomonas sp. KC8 OX=1030157 GN=KC8_00090 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.61GFRR19 pKa = 11.84SRR21 pKa = 11.84SATPGGRR28 pKa = 11.84KK29 pKa = 9.04VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3846

0

3846

1230456

29

4170

319.9

34.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.852 ± 0.061

0.763 ± 0.011

6.153 ± 0.03

5.061 ± 0.034

3.502 ± 0.023

9.115 ± 0.057

2.026 ± 0.02

5.327 ± 0.029

2.848 ± 0.027

9.667 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.478 ± 0.019

2.53 ± 0.031

5.31 ± 0.033

3.014 ± 0.022

7.383 ± 0.046

4.974 ± 0.03

5.293 ± 0.039

7.121 ± 0.032

1.39 ± 0.016

2.193 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski