Gloeocapsa sp. PCC 7428

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Chroococcaceae; Gloeocapsa; unclassified Gloeocapsa

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5003 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9XAJ2|K9XAJ2_9CHRO K+transporting ATPase F subunit OS=Gloeocapsa sp. PCC 7428 OX=1173026 GN=Glo7428_0029 PE=4 SV=1
MM1 pKa = 7.7AIIKK5 pKa = 8.02GTAGNDD11 pKa = 3.35NLKK14 pKa = 10.88GGSNNDD20 pKa = 2.97KK21 pKa = 10.71LYY23 pKa = 10.72GYY25 pKa = 10.06EE26 pKa = 5.79GEE28 pKa = 4.29DD29 pKa = 3.65TLNGGAGIDD38 pKa = 3.71TLIGGTGSDD47 pKa = 3.93VYY49 pKa = 10.98IVDD52 pKa = 3.54TTTDD56 pKa = 3.37VIIEE60 pKa = 4.04YY61 pKa = 10.37SGGGAHH67 pKa = 6.7DD68 pKa = 4.04TVKK71 pKa = 10.94SSVSYY76 pKa = 9.75TLGAYY81 pKa = 10.43VNDD84 pKa = 3.82LVLTGSSNINGTGNEE99 pKa = 4.37LDD101 pKa = 4.52NIITGNSGRR110 pKa = 11.84NVLRR114 pKa = 11.84GLAGNDD120 pKa = 3.28QLNGSNDD127 pKa = 3.11KK128 pKa = 11.09RR129 pKa = 11.84SDD131 pKa = 3.36TLYY134 pKa = 11.11GGVGNDD140 pKa = 3.4SLKK143 pKa = 11.15GGSKK147 pKa = 10.61DD148 pKa = 3.4KK149 pKa = 11.19LYY151 pKa = 11.32GGAGNDD157 pKa = 3.15RR158 pKa = 11.84LVGSHH163 pKa = 6.66VGFFDD168 pKa = 4.47NPDD171 pKa = 3.16DD172 pKa = 4.39TIYY175 pKa = 10.5MDD177 pKa = 4.65GGDD180 pKa = 4.08GNDD183 pKa = 3.52YY184 pKa = 10.99LYY186 pKa = 11.04ASHH189 pKa = 7.43DD190 pKa = 3.38AEE192 pKa = 4.64MYY194 pKa = 10.3GGRR197 pKa = 11.84GSDD200 pKa = 3.74TITGGTSVYY209 pKa = 10.27IDD211 pKa = 4.3GGDD214 pKa = 4.14GDD216 pKa = 5.46DD217 pKa = 5.68LLDD220 pKa = 4.27GDD222 pKa = 4.66EE223 pKa = 4.12STIYY227 pKa = 10.34GGKK230 pKa = 10.72GNDD233 pKa = 4.2TITGSYY239 pKa = 9.46SRR241 pKa = 11.84LYY243 pKa = 10.83GGDD246 pKa = 3.45GDD248 pKa = 5.45DD249 pKa = 3.92VLSGIAGMEE258 pKa = 4.07GGQGNDD264 pKa = 3.29TLIGGDD270 pKa = 3.46WDD272 pKa = 3.67SGFIFSKK279 pKa = 8.97PTDD282 pKa = 4.72GIDD285 pKa = 3.45TITNFAADD293 pKa = 4.9RR294 pKa = 11.84YY295 pKa = 9.03HH296 pKa = 7.13KK297 pKa = 10.15IYY299 pKa = 10.91VSASGFGGGLVEE311 pKa = 5.75GEE313 pKa = 4.61LLPGQLQFGTSATEE327 pKa = 3.6ADD329 pKa = 3.94DD330 pKa = 4.42RR331 pKa = 11.84FIYY334 pKa = 8.91DD335 pKa = 3.5TSSGALYY342 pKa = 10.51FDD344 pKa = 3.52VDD346 pKa = 3.99GVLGTGSQVQLAILVGVPDD365 pKa = 4.29LTSSSIYY372 pKa = 8.79VTSS375 pKa = 3.41

Molecular weight: 38.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9XCX1|K9XCX1_9CHRO HI0933 family protein OS=Gloeocapsa sp. PCC 7428 OX=1173026 GN=Glo7428_1367 PE=4 SV=1
MM1 pKa = 7.35SRR3 pKa = 11.84TRR5 pKa = 11.84RR6 pKa = 11.84HH7 pKa = 5.26RR8 pKa = 11.84NIRR11 pKa = 11.84AAQLFVIFAGITILVWVLRR30 pKa = 11.84GLGILTFLPGGILWILILLSVITGIWSRR58 pKa = 11.84FQRR61 pKa = 3.78

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5003

0

5003

1614380

29

3699

322.7

35.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.982 ± 0.036

0.974 ± 0.013

4.826 ± 0.029

5.963 ± 0.035

3.898 ± 0.024

6.586 ± 0.044

1.919 ± 0.018

6.556 ± 0.028

3.943 ± 0.033

11.003 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.798 ± 0.016

4.043 ± 0.03

4.805 ± 0.03

5.741 ± 0.041

5.507 ± 0.027

6.15 ± 0.025

5.869 ± 0.027

6.977 ± 0.027

1.42 ± 0.015

3.04 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski