Spiroplasma litorale

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1061 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1W276|A0A0K1W276_9MOLU Uncharacterized protein OS=Spiroplasma litorale OX=216942 GN=SLITO_v1c06420 PE=4 SV=1
MM1 pKa = 7.24NKK3 pKa = 9.76KK4 pKa = 9.93EE5 pKa = 4.06FFSIEE10 pKa = 4.0DD11 pKa = 3.29FRR13 pKa = 11.84EE14 pKa = 3.71FNDD17 pKa = 3.43YY18 pKa = 11.45DD19 pKa = 4.48NIMAQAFGVGCSLCGLEE36 pKa = 4.31EE37 pKa = 4.21IMYY40 pKa = 9.47TSINCPKK47 pKa = 10.46EE48 pKa = 3.26IGLVVKK54 pKa = 9.85EE55 pKa = 4.03IHH57 pKa = 7.35DD58 pKa = 4.32NNPNISDD65 pKa = 4.15SEE67 pKa = 4.24LDD69 pKa = 3.86SLLKK73 pKa = 10.82DD74 pKa = 4.87PIEE77 pKa = 4.3AWQEE81 pKa = 3.61VDD83 pKa = 5.24DD84 pKa = 4.32YY85 pKa = 10.96NSSIGAPTFLCIDD98 pKa = 4.84CYY100 pKa = 11.25DD101 pKa = 3.6QLISGEE107 pKa = 4.12IKK109 pKa = 10.5VSNIKK114 pKa = 10.49EE115 pKa = 3.98NN116 pKa = 3.4

Molecular weight: 13.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1W3P0|A0A0K1W3P0_9MOLU 50S ribosomal protein L34 OS=Spiroplasma litorale OX=216942 GN=rpmH PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.03IKK11 pKa = 10.14HH12 pKa = 5.53ARR14 pKa = 11.84THH16 pKa = 5.79GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.25NGRR28 pKa = 11.84KK29 pKa = 8.96VIKK32 pKa = 10.14ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.68GRR39 pKa = 11.84VKK41 pKa = 10.01LTAA44 pKa = 4.18

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1061

0

1061

360111

29

1525

339.4

38.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.268 ± 0.074

0.761 ± 0.019

5.566 ± 0.057

6.593 ± 0.074

5.596 ± 0.069

4.83 ± 0.079

1.093 ± 0.021

10.436 ± 0.095

10.776 ± 0.098

9.352 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.016 ± 0.029

8.534 ± 0.1

2.332 ± 0.04

2.48 ± 0.041

2.507 ± 0.045

6.948 ± 0.066

4.826 ± 0.055

5.588 ± 0.061

0.941 ± 0.023

4.558 ± 0.065
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski